中国神经精神疾病杂志
中國神經精神疾病雜誌
중국신경정신질병잡지
CHINESE JOURNAL OF NERVOUS AND MENTAL DISEASES
2015年
3期
141-144
,共4页
米诺环素%再灌注损伤%大鼠
米諾環素%再灌註損傷%大鼠
미낙배소%재관주손상%대서
Minocycline%Reperfusion injury%Rats
目的：探讨静脉用低剂量米诺环素对大鼠脑缺血再灌注损伤后神经功能恢复及排斥性导向分子（repulsive guidance molecule A, RGMa）表达的影响。方法成年雄性Sprague-Dawley大鼠54只，随机分为假手术组，缺血再灌注组和米诺环素组。采用大脑中动脉线栓法制作局灶性脑缺血再灌注模型。再灌注第2周，分别采用免疫组织化学及Western blot法检测缺血脑组织内RGMa及生长相关蛋白-43（growth associated pro?tein-43，GAP-43）蛋白的表达。缺血再灌注第2、7、14和28天，采用改良的神经功能缺损评分（modified neu?rological severity score, mNSS）及楼梯实验（staircase test）评估大鼠神经功能。结果同缺血再灌注组相比，低剂量米诺环素使缺血侧大脑皮层RGMa蛋白表达降低（0.53±0.08 vs.1.17±0.15, P<0.05），GAP-43蛋白表达明显增高（0.94±0.10 vs.0.57±0.09, P<0.05），大鼠mNSS评分显著下降并改善大鼠的前肢运动功能（P<0.05）。结论静脉用低剂量米诺环素（3 mg/kg）能促进大鼠缺血再灌注损伤后神经功能的恢复，其机制可能与下调RG?Ma蛋白及上调轴突再生相关蛋白GAP-43的表达有关。
目的：探討靜脈用低劑量米諾環素對大鼠腦缺血再灌註損傷後神經功能恢複及排斥性導嚮分子（repulsive guidance molecule A, RGMa）錶達的影響。方法成年雄性Sprague-Dawley大鼠54隻，隨機分為假手術組，缺血再灌註組和米諾環素組。採用大腦中動脈線栓法製作跼竈性腦缺血再灌註模型。再灌註第2週，分彆採用免疫組織化學及Western blot法檢測缺血腦組織內RGMa及生長相關蛋白-43（growth associated pro?tein-43，GAP-43）蛋白的錶達。缺血再灌註第2、7、14和28天，採用改良的神經功能缺損評分（modified neu?rological severity score, mNSS）及樓梯實驗（staircase test）評估大鼠神經功能。結果同缺血再灌註組相比，低劑量米諾環素使缺血側大腦皮層RGMa蛋白錶達降低（0.53±0.08 vs.1.17±0.15, P<0.05），GAP-43蛋白錶達明顯增高（0.94±0.10 vs.0.57±0.09, P<0.05），大鼠mNSS評分顯著下降併改善大鼠的前肢運動功能（P<0.05）。結論靜脈用低劑量米諾環素（3 mg/kg）能促進大鼠缺血再灌註損傷後神經功能的恢複，其機製可能與下調RG?Ma蛋白及上調軸突再生相關蛋白GAP-43的錶達有關。
목적：탐토정맥용저제량미낙배소대대서뇌결혈재관주손상후신경공능회복급배척성도향분자（repulsive guidance molecule A, RGMa）표체적영향。방법성년웅성Sprague-Dawley대서54지，수궤분위가수술조，결혈재관주조화미낙배소조。채용대뇌중동맥선전법제작국조성뇌결혈재관주모형。재관주제2주，분별채용면역조직화학급Western blot법검측결혈뇌조직내RGMa급생장상관단백-43（growth associated pro?tein-43，GAP-43）단백적표체。결혈재관주제2、7、14화28천，채용개량적신경공능결손평분（modified neu?rological severity score, mNSS）급루제실험（staircase test）평고대서신경공능。결과동결혈재관주조상비，저제량미낙배소사결혈측대뇌피층RGMa단백표체강저（0.53±0.08 vs.1.17±0.15, P<0.05），GAP-43단백표체명현증고（0.94±0.10 vs.0.57±0.09, P<0.05），대서mNSS평분현저하강병개선대서적전지운동공능（P<0.05）。결론정맥용저제량미낙배소（3 mg/kg）능촉진대서결혈재관주손상후신경공능적회복，기궤제가능여하조RG?Ma단백급상조축돌재생상관단백GAP-43적표체유관。
Objective To explore the effects of low dose intravenous minocycline on neurological function and the expression of RGMa in rats after focal cerebral ischemia reperfusion. Methods Fifty-five adult male Sprague-Dawley rats were randomly divided into sham-operated group, cerebral ischemia/reperfusion (I/R) group and minocycline-treat?ed group. The cerebral ischemia/reperfusion model was established by 2 h of middle cerebral artery occlusion. At 2 weeks after ischemia reperfusion, the expression levels of RGMa and growth associated protein 43 (GAP-43) were ana?lyzed by using immunohistochemistry and Western blot, respectively. Neurological functional recovery was evaluated us?ing both the modified neurological severity score (mNSS) and staircase test at 2, 7,14 and 28 d after ischemia reperfusion. Results Minocycline at a dose of 3 mg/kg via the caudal vein significantly reduced the expression of RGMa protein (0.53± 0.08 vs. 1.17±0.15, P<0.05) and enhanced the expression of GAP-43 protein (0.94±0.10 vs. 0.57±0.09, P<0.05) in isch?emic cortex 2 weeks after ischemia reperfusion. Moreover, minocycline could reduce mNSS and improve forelimb motor function when compared to the I/R group (P<0.05). Conclusions Low dose intravenous minocycline (3 mg/kg) can im?prove neurological functional recovery in a rat model of focal cerebral ischemia reperfusion and the mechanism may be re? lated to the down-regulation of RGMa expression and up-regulation of GAP-43 expression.
