干旱地区农业研究
榦旱地區農業研究
간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2015年
2期
106-112,134
,共8页
Zn2 +%PEG%纹党%透调节物质%活性氧代谢
Zn2 +%PEG%紋黨%透調節物質%活性氧代謝
Zn2 +%PEG%문당%투조절물질%활성양대사
Zn2 +%PEG%Codonopisis pilosula%osmotica%active oxygen metabolism
以甘肃道地中药材纹党(Codonopsis pilosula)为对象,通过室内砂培方法,研究了不同浓度(10-6 mol?L -1,10-5 mol?L -1,10-4 mol?L -1,10-3 mol?L -1) Zn2+和不同浓度(5%、10%、15%、20%、30%)PEG 模拟干旱胁迫下对纹党种子萌发、幼苗生长、渗透调节物质积累和活性氧代谢的影响。结果表明:低浓度 Zn2+和 PEG 能显著促进纹党种子萌发和幼苗生长,随着干旱胁迫和 Zn2+浓度的加剧,种子萌发率显著降低,幼苗生长受阻,纹党种子萌发和幼苗生长能耐受的渗透势临界阈值为-0.94 MPa ;叶片 O2?产生速率和 H2O2含量明显增加,SOD 、POD 、CAT和 APX 活性先升后降,高浓度 Zn2+和 PEG 处理下 GSH 和 AsA 含量明显降低,幼苗叶片中 Pro 、可溶性糖和 MDA 含量随 Zn2+和 PEG 浓度升高而升高。10-6和10-5 mol?L -1Zn 盐可提高纹党对适度干旱胁迫的适应能力,而10-3 mol?L -1的 Zn 盐与干旱胁迫下伤害度达到最大。
以甘肅道地中藥材紋黨(Codonopsis pilosula)為對象,通過室內砂培方法,研究瞭不同濃度(10-6 mol?L -1,10-5 mol?L -1,10-4 mol?L -1,10-3 mol?L -1) Zn2+和不同濃度(5%、10%、15%、20%、30%)PEG 模擬榦旱脅迫下對紋黨種子萌髮、幼苗生長、滲透調節物質積纍和活性氧代謝的影響。結果錶明:低濃度 Zn2+和 PEG 能顯著促進紋黨種子萌髮和幼苗生長,隨著榦旱脅迫和 Zn2+濃度的加劇,種子萌髮率顯著降低,幼苗生長受阻,紋黨種子萌髮和幼苗生長能耐受的滲透勢臨界閾值為-0.94 MPa ;葉片 O2?產生速率和 H2O2含量明顯增加,SOD 、POD 、CAT和 APX 活性先升後降,高濃度 Zn2+和 PEG 處理下 GSH 和 AsA 含量明顯降低,幼苗葉片中 Pro 、可溶性糖和 MDA 含量隨 Zn2+和 PEG 濃度升高而升高。10-6和10-5 mol?L -1Zn 鹽可提高紋黨對適度榦旱脅迫的適應能力,而10-3 mol?L -1的 Zn 鹽與榦旱脅迫下傷害度達到最大。
이감숙도지중약재문당(Codonopsis pilosula)위대상,통과실내사배방법,연구료불동농도(10-6 mol?L -1,10-5 mol?L -1,10-4 mol?L -1,10-3 mol?L -1) Zn2+화불동농도(5%、10%、15%、20%、30%)PEG 모의간한협박하대문당충자맹발、유묘생장、삼투조절물질적루화활성양대사적영향。결과표명:저농도 Zn2+화 PEG 능현저촉진문당충자맹발화유묘생장,수착간한협박화 Zn2+농도적가극,충자맹발솔현저강저,유묘생장수조,문당충자맹발화유묘생장능내수적삼투세림계역치위-0.94 MPa ;협편 O2?산생속솔화 H2O2함량명현증가,SOD 、POD 、CAT화 APX 활성선승후강,고농도 Zn2+화 PEG 처리하 GSH 화 AsA 함량명현강저,유묘협편중 Pro 、가용성당화 MDA 함량수 Zn2+화 PEG 농도승고이승고。10-6화10-5 mol?L -1Zn 염가제고문당대괄도간한협박적괄응능력,이10-3 mol?L -1적 Zn 염여간한협박하상해도체도최대。
A sand cultivation experiment was conducted to study the effects of Zn2 + with different concentrations (10 - 6 mol?L - 1 ,10 - 5 mol?L - 1 ,10 - 4 mol?L - 1 ,and 10 - 3 mol?L - 1 ) and PEG -6000 (5% 、10% 、15% 、20% 、30% ) on seed germination ,seedling growth ,osmotica accumulation ,and active oxygen metabolism of Codonopsis pilosula ,an authentic Chinese herbal medicine in Gansu province .The results showed that the low concentrations of Zn2 + and PEG could significantly enhance seed germination and seedling growth .With the increase of Zn2 + and PEG concentrations , the germination rate became decreased significantly ,and seedling growth was inhibited .The lowest permeability threshold was - 0 .94 MPa .O2 ? production rate and H2O2 contents in leaf were increased dramatically .In addition ,activities of superoxide dismutase ,peroxidase ,catalase ,and ascorbate peroxidase went down after an initial increase .Contents of glutathione and ascorbic acid in leaf were decreased markedly .Levels of proline ,soluble sugar and malonaldehyde con-tents in young seedling leaf went up significantly with the increase of Zn2 + and PEG concentrations .It was found that 10 - 6 and 10 - 5 mol?L - 1 zinc salt could improve the drought tolerance capability of Codonopsis pilosula to moderate stress .10 - 3 mol?L - 1 zinc salt and drought stress caused the maximal damages .