现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
7期
988-990
,共3页
脑缺血%再灌注损伤%苯丙胺%可卡因%神经肽类
腦缺血%再灌註損傷%苯丙胺%可卡因%神經肽類
뇌결혈%재관주손상%분병알%가잡인%신경태류
Brain ischemia%Reperfusion injury%Cocaine%Amphetamine%Neuropeptides
目的：探讨可卡因—苯丙胺调节转录肽（CARTp）对不同时间段脑缺血再灌注小鼠神经功能的影响。方法将成年美国癌症研究所雄性小鼠随机分为三组：假手术组（Sham组）、大脑中动脉阻塞组（MCAO组）和CARTp处理组，每组15只。采用线栓法复制MCAO模型。CARTp处理组小鼠于缺血再灌注第2天经尾静脉注射CARTp（2.5μg/kg），以后每天注射2.5μg/kg至第10天；Sham组、MCAO组注射生理盐水。通过神经功能评分判断神经功能障碍程度。观察并比较三组小鼠脑缺血再灌注损伤后的神经功能评分，判断不同处理组小鼠的神经功能障碍情况。结果 MCAO组和CARTp处理组神经功能评分均显著高于Sham组，差异均有统计学意义（P<0.05）；CARTp处理组的神经功能评分从第1天至第8天均低于MCAO组，差异均有统计学意义（P<0.05）。结论 CARTp能减轻小鼠脑缺血再灌注损伤引起的神经功能障碍，并可能在脑神经功能恢复中发挥重要作用，但随着时间延长，CARTp减轻神经功能障碍的作用明显减弱。
目的：探討可卡因—苯丙胺調節轉錄肽（CARTp）對不同時間段腦缺血再灌註小鼠神經功能的影響。方法將成年美國癌癥研究所雄性小鼠隨機分為三組：假手術組（Sham組）、大腦中動脈阻塞組（MCAO組）和CARTp處理組，每組15隻。採用線栓法複製MCAO模型。CARTp處理組小鼠于缺血再灌註第2天經尾靜脈註射CARTp（2.5μg/kg），以後每天註射2.5μg/kg至第10天；Sham組、MCAO組註射生理鹽水。通過神經功能評分判斷神經功能障礙程度。觀察併比較三組小鼠腦缺血再灌註損傷後的神經功能評分，判斷不同處理組小鼠的神經功能障礙情況。結果 MCAO組和CARTp處理組神經功能評分均顯著高于Sham組，差異均有統計學意義（P<0.05）；CARTp處理組的神經功能評分從第1天至第8天均低于MCAO組，差異均有統計學意義（P<0.05）。結論 CARTp能減輕小鼠腦缺血再灌註損傷引起的神經功能障礙，併可能在腦神經功能恢複中髮揮重要作用，但隨著時間延長，CARTp減輕神經功能障礙的作用明顯減弱。
목적：탐토가잡인—분병알조절전록태（CARTp）대불동시간단뇌결혈재관주소서신경공능적영향。방법장성년미국암증연구소웅성소서수궤분위삼조：가수술조（Sham조）、대뇌중동맥조새조（MCAO조）화CARTp처리조，매조15지。채용선전법복제MCAO모형。CARTp처리조소서우결혈재관주제2천경미정맥주사CARTp（2.5μg/kg），이후매천주사2.5μg/kg지제10천；Sham조、MCAO조주사생리염수。통과신경공능평분판단신경공능장애정도。관찰병비교삼조소서뇌결혈재관주손상후적신경공능평분，판단불동처리조소서적신경공능장애정황。결과 MCAO조화CARTp처리조신경공능평분균현저고우Sham조，차이균유통계학의의（P<0.05）；CARTp처리조적신경공능평분종제1천지제8천균저우MCAO조，차이균유통계학의의（P<0.05）。결론 CARTp능감경소서뇌결혈재관주손상인기적신경공능장애，병가능재뇌신경공능회복중발휘중요작용，단수착시간연장，CARTp감경신경공능장애적작용명현감약。
Objective To explore the effect of Cocaine—and amphetamine—regulated transcript(CARTp) on mice′s neuro—function after cerebral ischemia—reperfusion at different time periods. Methods Adult male Institute of Cancer Research (ICR) rats were randomly divided into the Sham operation group(the Sham group),the middle cerebral artery occlusion group(MCAO group) and the CARTp group,15 of each group. The transient middle cerebral artery occlusion (MCAO) rat model was per—formed by suture—occluded method. The 2.5μg/kg CARTp was injected to the mice in the CARTp group by vena caudalis on sec—ond day after MCAO and then 2.5 μg/kg daily till the tenth day while the Sham group and the MCAO group with normal saline. Neurological deficits degree were evaluated by Bederson′s score. It was observed and compared the neurological deficits after cerebral ischemia—reperfusion injury to judge the neurological deficits of the three rats groups. Results The neurological score of the MCAO group and the CARTp group was significantly higher than that of the Sham group ,which was statistically significant in difference (P<0.05). The neurological score of the CARTp group from the first day to the eighth day was lower than that of the MCAO group. The difference had statistical significance (P<0.05). Conclusion CARTp may relieve the neurological deficits af—ter cerebral ischemia—reperfusion,and also plays an important role in recovery of brain—neural functions.
