CAJ | 학술논문

目的：对华支睾吸虫硫氧还蛋白跨膜蛋白（CsTMX）基因进行生物信息学分析。方法利用生物信息学方法（InterProScan、SignalP、TMHMM和SWISS—MODEL等相关软件）对CsTMX基因及相应氨基酸序列的同源性、理化性质、保守结构域、信号肽、亲水性/疏水性、三级结构进行预测分析。结果 CsTMX基因的开放阅读框（ORF）包含414 bp，编码137个氨基酸，理论分子质量为16.04×103，等电点为5.16。TMHMM和SignalP3.0分析结果显示CsTMX含信号肽，其在第19~20位氨基酸之间有1个切割位点；InterProScan和SWISS—MODEL分析结果显示，TMX特征性结构域位于第21~27位氨基酸之间，并且具有高度保守的CPAC基因序列。结论利用生物信息学知识和各种分析软件对CsTMX所编码的cDNA序列及理论蛋白质的理化性质、结构和功能域等进行预测和分析，能够为开展CsTMX的表达及其功能研究提供理论依据。
목적：대화지고흡충류양환단백과막단백（CsTMX）기인진행생물신식학분석。방법이용생물신식학방법（InterProScan、SignalP、TMHMM화SWISS—MODEL등상관연건）대CsTMX기인급상응안기산서렬적동원성、이화성질、보수결구역、신호태、친수성/소수성、삼급결구진행예측분석。결과 CsTMX기인적개방열독광（ORF）포함414 bp，편마137개안기산，이론분자질량위16.04×103，등전점위5.16。TMHMM화SignalP3.0분석결과현시CsTMX함신호태，기재제19~20위안기산지간유1개절할위점；InterProScan화SWISS—MODEL분석결과현시，TMX특정성결구역위우제21~27위안기산지간，병차구유고도보수적CPAC기인서렬。결론이용생물신식학지식화각충분석연건대CsTMX소편마적cDNA서렬급이론단백질적이화성질、결구화공능역등진행예측화분석，능구위개전CsTMX적표체급기공능연구제공이론의거。
Objective To analyze protein characteristics of thioredoxin—related transmembrane protein gene of clonorchis sinensis(CsTMX) by bioinformation. Methods The homology,physicochemical property,conserved domain,signal peptide,hy—drophilcity,hydrophobicity and the tertiary structure of CsTMX and amino acid sequence were predicted by bioinformatic analysis software such as InterProScan,SignalP,TMHMM and SWISS—MODEL. Results The open reading frame of CsTMX cDNA in—cluded 414 bp,coded 137 amino acids with theoretical molecular weight being16.04 ×103 and isoelectric point being 5.16. TMH—MM and SignalP3.0 analysis showed that CsTMX contained signal peptide,having a cleavage site between 19th and 20th aminoacids. InterproScan and SWISS—MODEL analysis indicated the characterized TMX domain was found in the position of 21st-27th amino acids being possess with highly conserved CPAC gene sequence. Conclusion The bioinformatics analysis and vari—ous software may predict and analyze cDNA sequences coded by CsTMX and physicochemical property,structure and function do—main of theoretical protein,which is available for providing theoretical basis of expression of CsTMX.