CAJ | 학술논문

以火焰兰的茎段为外植体,探讨不同植物激素配比对茎段侧芽萌发和生根壮苗的影响,以及不同培养基配方和不同添加剂对火焰兰生长过程中褐化现象的抑制作用。结果表明：最佳侧芽诱导培养基为MS＋6-BA 3.0 mg/L＋NAA 0.5mg/L,培养40 d,后诱导率可达97.95%；添加活性炭0.3 g/L可有效抑制侧芽诱导过程中的褐化现象,褐化率仅为13.17%；1/2 MS＋6-BA 0.2 mg/L＋NAA 1.0 mg/L＋AC 0.5 g/L生根效果最好,生根率达到100%,且植株生长健壮。利用火焰兰茎段侧芽能够离体再生获得瓶苗,建立高效的火焰兰茎段为外植体的离体再生体系,为扩大拓宽火焰兰组织培养外植体来源和快速繁殖技术提供技术支撑。
이화염란적경단위외식체,탐토불동식물격소배비대경단측아맹발화생근장묘적영향,이급불동배양기배방화불동첨가제대화염란생장과정중갈화현상적억제작용。결과표명：최가측아유도배양기위MS＋6-BA 3.0 mg/L＋NAA 0.5mg/L,배양40 d,후유도솔가체97.95%；첨가활성탄0.3 g/L가유효억제측아유도과정중적갈화현상,갈화솔부위13.17%；1/2 MS＋6-BA 0.2 mg/L＋NAA 1.0 mg/L＋AC 0.5 g/L생근효과최호,생근솔체도100%,차식주생장건장。이용화염란경단측아능구리체재생획득병묘,건립고효적화염란경단위외식체적리체재생체계,위확대탁관화염란조직배양외식체래원화쾌속번식기술제공기술지탱。
The present study establishing efficient and stable Renanthera in vitro regeneration system by using stem buds, aim to expand the Renanthera explant sources and provide the technical support of rapid propagation technique of Renanthera. This research by using stem buds of Renanthera as explants, discusses the effect of different plant growth regulator combinations on the stem bud's inducing and rooting culture, and inhibitory effect of different medium and different additives on the growth process of Renanthera imschootiana Browning. Result: The optimal culture medium for stem bud inducement was MS+6-BA 3.0 mg/L+NAA 0.5mg/L, with the inductivity rate was 87.5% after 40 days of induction;adding activated carbon can control browning phenomenon of stem bud inducing culture effectively, with the browning rate was 13.17%; the optimal culture medium for rooting was 1/2MS+6-BA 0.2 mg/L+NAA 1.0mg/L+AC 0.5 g/L, with the rooting rate was 100%. Using the stem buds of Renanthera can in vitro regenerate plantlets, it's lay the foundation for the rapid propagation of Renanthera.