CAJ | 학술논문

目的：探讨神经肽Y(NPY)对原代小神经胶质细胞生物活性及生成IL-1β的影响。方法培养的原代大鼠皮层小胶质细胞，将细胞分为Control组、LPS组、NPY+LPS组、NPY组和BIBP3226+NPY+LPS组，每组3个样本，培养各组细胞6h。经免疫细胞化学荧光染色后，显微镜下观察小胶质细胞的形态学变化。Eli?sa方法检测培养液中IL-1β蛋白含量，RT-PCR方法检测小胶质细胞中IL-1βmRNA表达水平。结果孵育各组小胶质细胞6h后，LPS组培养液中IL-1β蛋白的含量及细胞中IL-1βmRNA表达水平分别为（961.00±83.50）pg/mL和5.59±0.87，显著高于Control组的96.33±24.58 pg/mL和1.05±0.12（P＜0.05），小胶质细胞处于活化状态；LPS+NPY组IL-1β蛋白含量和mRNA表达水平分别为(411.33±55.00)pg/mL和1.93±0.45，与LPS组相比显著降低（P＜0.05），小胶质细胞活化水平降低；IBP3226+NPY+LPS组IL-1β蛋白含量和mRNA表达水平分别为(886.00±97.53)pg/mL和4.51±0.71，与LPS+NPY组相比显著增高（P＜0.05）；LPS组和IBP3226+NPY+LPS组之间无统计学意义。NPY组与对照组无统计学意义。结论 NPY通过作用于NPY Y1受体降低小神经胶质细胞的生物活性，抑制其生成IL-1β。
목적：탐토신경태Y(NPY)대원대소신경효질세포생물활성급생성IL-1β적영향。방법배양적원대대서피층소효질세포，장세포분위Control조、LPS조、NPY+LPS조、NPY조화BIBP3226+NPY+LPS조，매조3개양본，배양각조세포6h。경면역세포화학형광염색후，현미경하관찰소효질세포적형태학변화。Eli?sa방법검측배양액중IL-1β단백함량，RT-PCR방법검측소효질세포중IL-1βmRNA표체수평。결과부육각조소효질세포6h후，LPS조배양액중IL-1β단백적함량급세포중IL-1βmRNA표체수평분별위（961.00±83.50）pg/mL화5.59±0.87，현저고우Control조적96.33±24.58 pg/mL화1.05±0.12（P＜0.05），소효질세포처우활화상태；LPS+NPY조IL-1β단백함량화mRNA표체수평분별위(411.33±55.00)pg/mL화1.93±0.45，여LPS조상비현저강저（P＜0.05），소효질세포활화수평강저；IBP3226+NPY+LPS조IL-1β단백함량화mRNA표체수평분별위(886.00±97.53)pg/mL화4.51±0.71，여LPS+NPY조상비현저증고（P＜0.05）；LPS조화IBP3226+NPY+LPS조지간무통계학의의。NPY조여대조조무통계학의의。결론 NPY통과작용우NPY Y1수체강저소신경효질세포적생물활성，억제기생성IL-1β。
Objective To explore the effect of NPY on activation of primary microglia and the production of in?terleukin-1β. Methods Rat primary cortical microglia was cultured and divided into control group, LPS group, NPY+LPS group, NPY group and BIBP3226+NPY+LPS group. Microglia in control group were incubated with serum-free me?dium for 6 h;microglia in LPS group were incubated with serum-free medium plus LPS for 6 h;microglia in NPY+LPS group were incubated with serum-free medium plus NPY and LPS for 6 h; microglia cells in NPY group were incubat?ed in serum-free medium plus NPY for 6 h; microglia cells in BIBP3226+NPY+LPS group were incubated in se?rum-free medium including BIBP3226 、NPY and LPS for 6 h. After 6 h , Primary cultured microglia were stained us?ing IBA-1 antibody and examined under the fluorescence microscope. The protein levels of IL-1βin the culture media and the mRNA expression levels of IL-1βin the microglia of different groups were detected using the methods of Elisa and RT-PCR. Results After 6 h, the contents of IL-1 βin the culture media and the mRNA expression levels of IL-1βin the cells of LPS group increased remarkably compared with control group (P<0.05) and the microglia were activat? ed. Compared with LPS group, the contents of IL-1 βin the culture media. the mRNA expression levels of IL-1β and the activity of microglia in LPS+NPY group were significantly decreased .Compared with LPS+NPY group, the contents of IL-1βin the culture media. the mRNA expression levels of IL-1β and the activity of microglia in BIBP3226+NPY+LPS group were increased (P<0.05). There were no significant differences in the contents of IL-1βin the culture media. the mRNA expression levels of IL-1βand the activity of microglia between BIBP3226+NPY+LPS group and LPS group or between NPY group and the control group. Conclusion NPY can inhibit the biological activity of microglia and IL-1βproduction through NPY Y1 receptorin the microglia.