CAJ | 학술논문

建立了鸡肉中环丙沙星的高效液相色谱串联质谱测定方法，试样经 EDTA-Na缓冲盐提取和净化后，C18柱HPLC进行分离，多反应选择离子检测。方法的回收率和变异系数分别在90%～110%和3%～10%。该方法对鸡肉中环丙沙星检测限为1μg/kg。
건립료계육중배병사성적고효액상색보천련질보측정방법，시양경 EDTA-Na완충염제취화정화후，C18주HPLC진행분리，다반응선택리자검측。방법적회수솔화변이계수분별재90%～110%화3%～10%。해방법대계육중배병사성검측한위1μg/kg。
A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS /MS) method was developed for the quantitative determination of Ciprofloxacin respectively in the chicken. The ciprofloxacin in chicken were deproteinated with EDTA-Na Mcllvaine buffer followed by liquid-liquid extraction. Further clean-up was performed by solid phase extraction (SPE) through mixed mode columns (PEP-2 SPE). The separation of the compounds was carried on a Agilent Eclipse Plus C18 column (C18, 3.5μm× 100 mm×2.1 mm(id) by a gradient elution using 0.1%formic acid acetonitrile and 0.1%formic acid water as mobile phases. The analyzes were detected by tandem mass spectrometry in multiple reaction monitoring (MRM) mode with negative electrospray ioniza﹣tion. The ciprofloxacin quantified by internal standard method. The linear ranges were 1.0~20.0 μg/L and the correlation coefficients were not less than 0.992. The average recoveries and relative standard deviations ranged from90%~110%and 3%~10%respectively at the spiked levels of 1,2 and 10 μg/kg for the both markers. The limit of quantitation (LOQ) was 1 μg/kg. The method is sensitive,ac﹣curate and suitable for the determination and confirmation of chloramphenicols in chicken and eggs.