CAJ | 학술논문

目的：探讨胶质瘤相关性癫痫患者瘤组织中高迁移率族蛋白B1(High-mobility group box-1， HMGB1)的表达。方法利用免疫组化半定量法检测82例胶质瘤相关性癫痫患者（胶质瘤并癫痫组）胶质瘤组织、80例胶质瘤不伴发癫痫的患者（胶质瘤非癫痫组）胶质瘤组织、80例难治性癫痫患者（非肿瘤癫痫组）致痫灶组织、20例行颅内减压术患者（阴性对照组）的正常脑组织中HMGB1的表达情况，统计分析实验组中HMGB1的表达及其临床意义。结果胶质瘤并癫痫组胶质瘤组织中HMGB1表达高于胶质瘤非癫痫组中胶质瘤组织(χ2=16.944, P<0.001)，低级别胶质瘤尤甚；高于非肿瘤癫痫组中致痫灶组织(χ2=26.094, P<0.001)。胶质瘤非癫痫组胶质瘤组织中HMGB1的表达(χ2=32.273, P<0.001)、非肿瘤癫痫组致痫灶组织中HMGB1的表达(χ2=22.236，P<0.001)均高于阴性对照组中正常脑组织。胶质瘤并癫痫组中HMGB1的表达与胶质瘤相关性癫痫的持续时间(r=0.365, P=0.001)、发作频率(r=0.531, P<0.001)、胶质瘤的病理级别(r=0.265, P=0.016)呈正相关。结论 HMGB1在胶质瘤相关性癫痫患者胶质瘤组织中高表达，且与癫痫发作情况关系密切，胶质瘤组织中HMGB1可能与其相关性癫痫的形成有关。
목적：탐토효질류상관성전간환자류조직중고천이솔족단백B1(High-mobility group box-1， HMGB1)적표체。방법이용면역조화반정량법검측82례효질류상관성전간환자（효질류병전간조）효질류조직、80례효질류불반발전간적환자（효질류비전간조）효질류조직、80례난치성전간환자（비종류전간조）치간조조직、20례행로내감압술환자（음성대조조）적정상뇌조직중HMGB1적표체정황，통계분석실험조중HMGB1적표체급기림상의의。결과효질류병전간조효질류조직중HMGB1표체고우효질류비전간조중효질류조직(χ2=16.944, P<0.001)，저급별효질류우심；고우비종류전간조중치간조조직(χ2=26.094, P<0.001)。효질류비전간조효질류조직중HMGB1적표체(χ2=32.273, P<0.001)、비종류전간조치간조조직중HMGB1적표체(χ2=22.236，P<0.001)균고우음성대조조중정상뇌조직。효질류병전간조중HMGB1적표체여효질류상관성전간적지속시간(r=0.365, P=0.001)、발작빈솔(r=0.531, P<0.001)、효질류적병리급별(r=0.265, P=0.016)정정상관。결론 HMGB1재효질류상관성전간환자효질류조직중고표체，차여전간발작정황관계밀절，효질류조직중HMGB1가능여기상관성전간적형성유관。
Objective To explore expression of HMGB1 in glioma tissue of glioma-related epilepsy patients. Methods Immunohistochemistry was used to detect the expression of HMGB1 in the tissues from 82 glioma-related epi?lepsy patients (glioma-related epilepsy group), 80 glioma patients (glioma without epilepsy group), 80 intractable epilepsy patients (epilepsy control group) epileptogenic foci tissue and 20 normal controls (negative control group). Results HMGB1 in glioma tissue of glioma-related epilepsy group was significantly higher than that in glioma tissue of glioma without epilepsy grou p (χ2=16.944, P<0.001), especially in low pathological grade glioma tissue. HMGB1 was higher in glioma tissue of glioma-related epilepsy group than in epileptogenic foci tissue of epilepsy control group (χ2=26.094, P<0.001). Expression of HMGB1 in glioma tissue of glioma without epilepsy group (χ2=32.273, P<0.001) and epileptogenic foci tissue of epilepsy control group ( χ2=22.236,P<0.001) was higher than in normal brain tissue of negative control group. In glioma-related epilepsy group, HMGB1 was positively correlated with seizures duration(r=0.365,P=0.001), sei? zures frequency (r=0.531,P=0.000) and pathological grade of glioma tissue (r=0.265,P=0.016). Conclusions HMGB1 is highly expressed in glioma tissues of glioma-related epilepsy; HMGB1 expression is closely related with seizures; and HMGB1 in glioma tissue may contribute to the formation of glioma-related epilepsy.