中国实验方剂学杂志
中國實驗方劑學雜誌
중국실험방제학잡지
CHINESE JOURNAL OF EXPERIMENTAL TRADITIONAL MEDICAL FORMULAE
2015年
6期
48-51
,共4页
候金燕%罗琳%窦志华%冯艳%陆群%宣东平%陈霞%顾薇
候金燕%囉琳%竇誌華%馮豔%陸群%宣東平%陳霞%顧薇
후금연%라림%두지화%풍염%륙군%선동평%진하%고미
茵陈蒿汤%儿茶素%绿原酸%西红花苷Ⅰ%西红花苷Ⅱ%含量测定
茵陳蒿湯%兒茶素%綠原痠%西紅花苷Ⅰ%西紅花苷Ⅱ%含量測定
인진호탕%인다소%록원산%서홍화감Ⅰ%서홍화감Ⅱ%함량측정
Yinchenhao decoction%catechin%chlorogenic acid%crocin-Ⅰ%crocin-Ⅱ%determination
目的:建立HPLC-DAD测定茵陈蒿汤中儿茶素、绿原酸、西红花苷Ⅰ、西红花苷Ⅱ含量的方法.方法:采用Symmetry C18色谱柱(4.6 mm×250mm,5μm),流动相乙腈-0.1％磷酸水梯度洗脱,流速1.0 mL· min-1,柱温30℃,儿茶素检测波长202 nm,绿原酸检测波长330 nm,西红花苷Ⅰ,Ⅱ检测波长440 nm.结果:儿茶素、绿原酸、西红花苷Ⅰ,Ⅱ分别在0.18 ～0.9(r =0.999 8),0.062 4 ～0.312(r =0.999 9),0.441 ～2.205(r =0.999 9),0.023 52 ～0.117 6μg(r =0.999 9)与峰面积呈良好线性关系;加样回收率分别为98.56％ (RSD 1.8％),99.07％ (RSD 1.8％),97.41％ (RSD 1.9％)和98.46％(RSD1.9％).结论:该法简便、准确、专属性强,可用于茵陈蒿汤中4种成分的含量测定.
目的:建立HPLC-DAD測定茵陳蒿湯中兒茶素、綠原痠、西紅花苷Ⅰ、西紅花苷Ⅱ含量的方法.方法:採用Symmetry C18色譜柱(4.6 mm×250mm,5μm),流動相乙腈-0.1％燐痠水梯度洗脫,流速1.0 mL· min-1,柱溫30℃,兒茶素檢測波長202 nm,綠原痠檢測波長330 nm,西紅花苷Ⅰ,Ⅱ檢測波長440 nm.結果:兒茶素、綠原痠、西紅花苷Ⅰ,Ⅱ分彆在0.18 ～0.9(r =0.999 8),0.062 4 ～0.312(r =0.999 9),0.441 ～2.205(r =0.999 9),0.023 52 ～0.117 6μg(r =0.999 9)與峰麵積呈良好線性關繫;加樣迴收率分彆為98.56％ (RSD 1.8％),99.07％ (RSD 1.8％),97.41％ (RSD 1.9％)和98.46％(RSD1.9％).結論:該法簡便、準確、專屬性彊,可用于茵陳蒿湯中4種成分的含量測定.
목적:건립HPLC-DAD측정인진호탕중인다소、록원산、서홍화감Ⅰ、서홍화감Ⅱ함량적방법.방법:채용Symmetry C18색보주(4.6 mm×250mm,5μm),류동상을정-0.1％린산수제도세탈,류속1.0 mL· min-1,주온30℃,인다소검측파장202 nm,록원산검측파장330 nm,서홍화감Ⅰ,Ⅱ검측파장440 nm.결과:인다소、록원산、서홍화감Ⅰ,Ⅱ분별재0.18 ～0.9(r =0.999 8),0.062 4 ～0.312(r =0.999 9),0.441 ～2.205(r =0.999 9),0.023 52 ～0.117 6μg(r =0.999 9)여봉면적정량호선성관계;가양회수솔분별위98.56％ (RSD 1.8％),99.07％ (RSD 1.8％),97.41％ (RSD 1.9％)화98.46％(RSD1.9％).결론:해법간편、준학、전속성강,가용우인진호탕중4충성분적함량측정.
