中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2015年
1期
37-40,53
,共5页
万金娥%季翠杰%谷健%王春莲%仲蓓%徐晶%王培嵩
萬金娥%季翠傑%穀健%王春蓮%仲蓓%徐晶%王培嵩
만금아%계취걸%곡건%왕춘련%중배%서정%왕배숭
大鼠%卵巢%高压氧%骨密度%骨生物力学%骨质疏松
大鼠%卵巢%高壓氧%骨密度%骨生物力學%骨質疏鬆
대서%란소%고압양%골밀도%골생물역학%골질소송
Rat%Ovary%Hyperbaric oxygen%Bone mineral density%Bone biomechanics%Osteoporosis
目的 观察高压氧干预对去卵巢实验大鼠骨质疏松的影响.方法 6月龄Wister雌性大鼠40只,按数字表法随机分为5组:假手术组、去卵巢组、雌激素组、高压氧组、高气压组,每组8只.除假手术组外,其余各组麻醉后切除双侧卵巢.1周后,假手术组和去卵巢组不加任何干预,高压氧组每日高压氧(表压0.1 MPa)暴露1次,每10次休息1周,共暴露12周;高气压组以压缩空气为加压介质在高压空气中暴露,其他条件和方法同高压氧组;雌激素组给予苯甲酸雌二醇腹腔注射,每周1次,共12周.实验结束后检测各组大鼠腰椎及股骨的骨密度、骨生物力学、骨形态计量学等指标.结果 去卵巢后,大鼠腰椎骨密度为(0.087 8 ±0.007 4)g/cm2,股骨密度为(0.114 1 ±0.006 7)g/cm2,腰椎骨最大应力为(216.32±45.26)N,股骨最大应力为(87.50 ±4.01)N,均较假手术组[分别为(0.123 0 ±0.087 8)g/cm2、(0.129 0 ±0.034 0)g/cm2、(381.58±28.75)N、(120.01±16.26)N]明显降低,差异有统计学意义(P<0.05);高压氧组以上各指标下降均不明显,与假手术组比较差异无统计学意义(P>0.05),与高气压组比较差异有统计学意义(P<0.05).结论 高压氧可以减轻去卵巢大鼠的骨密度和骨生物力学特性的降低,对绝经后骨质疏松的发生有一定的预防作用.
目的 觀察高壓氧榦預對去卵巢實驗大鼠骨質疏鬆的影響.方法 6月齡Wister雌性大鼠40隻,按數字錶法隨機分為5組:假手術組、去卵巢組、雌激素組、高壓氧組、高氣壓組,每組8隻.除假手術組外,其餘各組痳醉後切除雙側卵巢.1週後,假手術組和去卵巢組不加任何榦預,高壓氧組每日高壓氧(錶壓0.1 MPa)暴露1次,每10次休息1週,共暴露12週;高氣壓組以壓縮空氣為加壓介質在高壓空氣中暴露,其他條件和方法同高壓氧組;雌激素組給予苯甲痠雌二醇腹腔註射,每週1次,共12週.實驗結束後檢測各組大鼠腰椎及股骨的骨密度、骨生物力學、骨形態計量學等指標.結果 去卵巢後,大鼠腰椎骨密度為(0.087 8 ±0.007 4)g/cm2,股骨密度為(0.114 1 ±0.006 7)g/cm2,腰椎骨最大應力為(216.32±45.26)N,股骨最大應力為(87.50 ±4.01)N,均較假手術組[分彆為(0.123 0 ±0.087 8)g/cm2、(0.129 0 ±0.034 0)g/cm2、(381.58±28.75)N、(120.01±16.26)N]明顯降低,差異有統計學意義(P<0.05);高壓氧組以上各指標下降均不明顯,與假手術組比較差異無統計學意義(P>0.05),與高氣壓組比較差異有統計學意義(P<0.05).結論 高壓氧可以減輕去卵巢大鼠的骨密度和骨生物力學特性的降低,對絕經後骨質疏鬆的髮生有一定的預防作用.
목적 관찰고압양간예대거란소실험대서골질소송적영향.방법 6월령Wister자성대서40지,안수자표법수궤분위5조:가수술조、거란소조、자격소조、고압양조、고기압조,매조8지.제가수술조외,기여각조마취후절제쌍측란소.1주후,가수술조화거란소조불가임하간예,고압양조매일고압양(표압0.1 MPa)폭로1차,매10차휴식1주,공폭로12주;고기압조이압축공기위가압개질재고압공기중폭로,기타조건화방법동고압양조;자격소조급여분갑산자이순복강주사,매주1차,공12주.실험결속후검측각조대서요추급고골적골밀도、골생물역학、골형태계량학등지표.결과 거란소후,대서요추골밀도위(0.087 8 ±0.007 4)g/cm2,고골밀도위(0.114 1 ±0.006 7)g/cm2,요추골최대응력위(216.32±45.26)N,고골최대응력위(87.50 ±4.01)N,균교가수술조[분별위(0.123 0 ±0.087 8)g/cm2、(0.129 0 ±0.034 0)g/cm2、(381.58±28.75)N、(120.01±16.26)N]명현강저,차이유통계학의의(P<0.05);고압양조이상각지표하강균불명현,여가수술조비교차이무통계학의의(P>0.05),여고기압조비교차이유통계학의의(P<0.05).결론 고압양가이감경거란소대서적골밀도화골생물역학특성적강저,대절경후골질소송적발생유일정적예방작용.
Objective To observe effect of hyperbaric oxygen (HBO) intervention on osteoporosis in rats with experimental premature ovary failureMethods Forty female Wister rats (with an age of 6 weeks) were randomly divided into 5 groups:the sham group,the deovary group,the estrin group,the HBO group and the hyperbaric air group,each consisting of 8 animals.With the exception of the sham group,all the other groups had ovariotomy bilaterally following anesthesia.One week later,the animals in the sham group and the deovary group were left there without any treatment,while the animals in the HBO group were exposed to HBO at a pressure of 0.1 MPa,one session a day for a succession of 10 days,then had one week interval every 10 sessions,with a total exposure time of 12 weeks.The animals in the hyperbaric air group were exposed to hyperbaric air at the same pressure and with the same treatment time and conditions.The animals in the estrin group received abdominal injection of estradiol benzoate once a week,for a succession of 12 weeks.After termination of the experiment,the animals of all the groups received lumbar vertebra and femora mineral density,bonc biomechanics and bone morphometry detection.Results After ovariotomy,lumbar vertebra mineral density was (0.087 8 ± 0.007 4) g/cm2 and femora mineral density was (0.114 1 ± 0.006 7) g/cm2.Lumbar vertebra biomechanics (216.32 ± 45.26) N and femora biomechanics (87.50 ± 4.01) N were significantly decreased,and statistical significance could be seen,as compared with those of the sham group [(381.58 ±28.75) N and(120 ± 16.26) N] (P < 0.05).However,the levels of the above indicators did not reduce considerably and there was no significant difference,as compared with those of the sham group (P > 0.05),but there was obviously significant difference in them,when it was compared with that of the hyperbaric air group (P > 0.05).Conclusions HBO could delay the loss of bone mineral density and the reduced property of bone biomechanics in ovarectomized rats,and it had certain protective effect on the occurrence of osteoporosis after menopause.