中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2015年
1期
27-31
,共5页
王宇%吴勇%许冀%刘煜敏
王宇%吳勇%許冀%劉煜敏
왕우%오용%허기%류욱민
高压氧%阿尔茨海默病%脑源性神经营养因子%酪氨酸激酶受体B%突触素
高壓氧%阿爾茨海默病%腦源性神經營養因子%酪氨痠激酶受體B%突觸素
고압양%아이자해묵병%뇌원성신경영양인자%락안산격매수체B%돌촉소
Hyperbaric oxygen%Alzheimer's disease%Brain-derived neurotrophic factor%Tyrosine kinase receptor B%Synaptophysin
目的 探讨高压氧(hyperbaric oxygen,HBO)治疗阿尔茨海默病(AD)模型大鼠后其认知和记忆功能的改变及其与海马组织中脑源性神经营养因子(BDNF)、酪氨酸激酶受体B(TrkB)以及突触素表达水平的关系.方法 将40只SD大鼠按数字表法随机分为4组:正常对照组、假手术组、模型对照组和HBO治疗组,每组10只,分别进行相应的海马注射处理,实验期间每组给予常规清洁饮食,保持恒温.Aβ25-35注射2周后行HBO治疗,治疗结束后各组大鼠分别行Morris水迷宫实验,包括定位航行实验和空间探索实验,检测其空间记忆能力的改变情况.然后观察各组大鼠海马组织中BDNF,TrkB以及突触素的表达情况.结果 Morris水迷宫实验显示,HBO组大鼠穿越原平台次数(3.3次)、在原平台象限游泳时间所占百分比(30％)与模型对照组(分别为2.2次和15％)比较差异有统计学意义(P＜0.05),表明HBO能够显著改善AD大鼠模型的空间记忆能力,HBO组海马组织中BDNF、TrkB以及突触素的表达与模型对照组比较明显提高,差异有统计学意义(P＜0.05).结论 HBO可能通过增加与记忆密切相关的BDNF及其下游的TrkB和突触素的表达从而改善AD大鼠模型的认知和记忆能力.
目的 探討高壓氧(hyperbaric oxygen,HBO)治療阿爾茨海默病(AD)模型大鼠後其認知和記憶功能的改變及其與海馬組織中腦源性神經營養因子(BDNF)、酪氨痠激酶受體B(TrkB)以及突觸素錶達水平的關繫.方法 將40隻SD大鼠按數字錶法隨機分為4組:正常對照組、假手術組、模型對照組和HBO治療組,每組10隻,分彆進行相應的海馬註射處理,實驗期間每組給予常規清潔飲食,保持恆溫.Aβ25-35註射2週後行HBO治療,治療結束後各組大鼠分彆行Morris水迷宮實驗,包括定位航行實驗和空間探索實驗,檢測其空間記憶能力的改變情況.然後觀察各組大鼠海馬組織中BDNF,TrkB以及突觸素的錶達情況.結果 Morris水迷宮實驗顯示,HBO組大鼠穿越原平檯次數(3.3次)、在原平檯象限遊泳時間所佔百分比(30％)與模型對照組(分彆為2.2次和15％)比較差異有統計學意義(P＜0.05),錶明HBO能夠顯著改善AD大鼠模型的空間記憶能力,HBO組海馬組織中BDNF、TrkB以及突觸素的錶達與模型對照組比較明顯提高,差異有統計學意義(P＜0.05).結論 HBO可能通過增加與記憶密切相關的BDNF及其下遊的TrkB和突觸素的錶達從而改善AD大鼠模型的認知和記憶能力.
목적 탐토고압양(hyperbaric oxygen,HBO)치료아이자해묵병(AD)모형대서후기인지화기억공능적개변급기여해마조직중뇌원성신경영양인자(BDNF)、락안산격매수체B(TrkB)이급돌촉소표체수평적관계.방법 장40지SD대서안수자표법수궤분위4조:정상대조조、가수술조、모형대조조화HBO치료조,매조10지,분별진행상응적해마주사처리,실험기간매조급여상규청길음식,보지항온.Aβ25-35주사2주후행HBO치료,치료결속후각조대서분별행Morris수미궁실험,포괄정위항행실험화공간탐색실험,검측기공간기억능력적개변정황.연후관찰각조대서해마조직중BDNF,TrkB이급돌촉소적표체정황.결과 Morris수미궁실험현시,HBO조대서천월원평태차수(3.3차)、재원평태상한유영시간소점백분비(30％)여모형대조조(분별위2.2차화15％)비교차이유통계학의의(P＜0.05),표명HBO능구현저개선AD대서모형적공간기억능력,HBO조해마조직중BDNF、TrkB이급돌촉소적표체여모형대조조비교명현제고,차이유통계학의의(P＜0.05).결론 HBO가능통과증가여기억밀절상관적BDNF급기하유적TrkB화돌촉소적표체종이개선AD대서모형적인지화기억능력.
Objective To explore changes in cognitive and memory function in the Alzheimer's disease (AD) model rats,and also its association with the changes in the expressions of brain-derived neurotrophic factor (BDNF),tyrosine kinase receptor B (TrkB) and synaptophysin in hippocampus,following HBO treatment.Methods The rats were randomly divided into 4 groups:the normal control group,the sham surgical group (5μ1 of saline were injected into bilateral hippocampus),the model control group (5μl of Aβ25-35 were injected into bilateral hippocampus) and the HBO treatment group (5μl of Aβ25-35 were injected into bilateral hippocampus and treated with HBO),each consisting of 10 animals.During the experiment,the rats were fed with conventional clean food and were kept in a space with constant temperature.Two weeks after injection with Aβ25-35,the rats were treated with HBO.Following completion of treatment,the rats of all the groups had the Morris water maze tests,i.e.navigational experiment and space exploration experiment,to detect changes in cognitive and spatial memory.Then,the expression levels of BDNF,TrkB and synaptophys in hippocampus were detected by Western Blot and immunohistochemistry.Results The results of the Morris water maze tests indicated that the number of running across the platform for the rats in the HBO group was 3.3 and the percentage of swimming in the original quadrant accounted for 30％,and statistical significance could be seen when comparisons were made (P ＜ 0.05).It was suggested that HBO could significantly improve spatial memory of the AD model rats,and at the same time it could enhance the expressions of BDNF,TrkB and synaptophysin in hippocampus.Statistical significance could be noted when it was compared with the model control group (P ＜ 0.05).Conclusions HBO might increase the expression levels of BDNF,TrkB and synaptophysin,which were closely associated with memory,thus improving the cognitive function and spatial memory of the AD model rats.
