中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2015年
2期
87-90
,共4页
王晓晨%张欣%陈天石%陈佰义
王曉晨%張訢%陳天石%陳佰義
왕효신%장흔%진천석%진백의
肠球菌%接种效应%磷霉素
腸毬菌%接種效應%燐黴素
장구균%접충효응%린매소
Enterococcus%Inoculum effect%Fosfomycin
目的 与青霉素、利福平、万古霉素、替考拉宁等4种常用抗菌药物对比研究,分析磷霉素对肠球菌的接种效应.方法 选择2010年1月至2012年1月自中国医科大学附属第一医院住院患者分离的91株肠球菌,均为不重复菌株,包括粪肠球菌44株,屎肠球菌47株.采用琼脂稀释法测定磷霉素、青霉素、利福平、万古霉素、替考拉宁5种抗菌药物对91株肠球菌在低接种浓度[1×105菌落形成单位(cfu)/mL]和高接种浓度(1×107 cfu/mL)下的最低抑菌浓度(MIC).结果 对于所有受试菌株,磷霉素在高接种浓度、低接种浓度下的MIC值均波动在16～64 μg/mL之间.受试菌株,不管是粪肠球菌,还是屎肠球菌,均对磷霉素的敏感率最高(100.0％),耐药率最低(0).磷霉素、青霉素、利福平、万古霉素、替考拉宁5种抗菌药物随着菌株接种浓度的增加,其菌株MIC值均升高不足8倍,即均不存在接种效应.结论 对于肠球菌引起的感染,由于磷霉素体外药物敏感试验显示其敏感性高,且不存在接种效应,因此可用于临床治疗.
目的 與青黴素、利福平、萬古黴素、替攷拉寧等4種常用抗菌藥物對比研究,分析燐黴素對腸毬菌的接種效應.方法 選擇2010年1月至2012年1月自中國醫科大學附屬第一醫院住院患者分離的91株腸毬菌,均為不重複菌株,包括糞腸毬菌44株,屎腸毬菌47株.採用瓊脂稀釋法測定燐黴素、青黴素、利福平、萬古黴素、替攷拉寧5種抗菌藥物對91株腸毬菌在低接種濃度[1×105菌落形成單位(cfu)/mL]和高接種濃度(1×107 cfu/mL)下的最低抑菌濃度(MIC).結果 對于所有受試菌株,燐黴素在高接種濃度、低接種濃度下的MIC值均波動在16～64 μg/mL之間.受試菌株,不管是糞腸毬菌,還是屎腸毬菌,均對燐黴素的敏感率最高(100.0％),耐藥率最低(0).燐黴素、青黴素、利福平、萬古黴素、替攷拉寧5種抗菌藥物隨著菌株接種濃度的增加,其菌株MIC值均升高不足8倍,即均不存在接種效應.結論 對于腸毬菌引起的感染,由于燐黴素體外藥物敏感試驗顯示其敏感性高,且不存在接種效應,因此可用于臨床治療.
목적 여청매소、리복평、만고매소、체고랍저등4충상용항균약물대비연구,분석린매소대장구균적접충효응.방법 선택2010년1월지2012년1월자중국의과대학부속제일의원주원환자분리적91주장구균,균위불중복균주,포괄분장구균44주,시장구균47주.채용경지희석법측정린매소、청매소、리복평、만고매소、체고랍저5충항균약물대91주장구균재저접충농도[1×105균락형성단위(cfu)/mL]화고접충농도(1×107 cfu/mL)하적최저억균농도(MIC).결과 대우소유수시균주,린매소재고접충농도、저접충농도하적MIC치균파동재16～64 μg/mL지간.수시균주,불관시분장구균,환시시장구균,균대린매소적민감솔최고(100.0％),내약솔최저(0).린매소、청매소、리복평、만고매소、체고랍저5충항균약물수착균주접충농도적증가,기균주MIC치균승고불족8배,즉균불존재접충효응.결론 대우장구균인기적감염,유우린매소체외약물민감시험현시기민감성고,차불존재접충효응,인차가용우림상치료.
Objective To investigate the inoculum effect of fosfomycin on enterococci,in comparison with penicillin,rifampin,vancomycin and teicoplanin.Methods A total of 91 strains of enterococci were obtained from the patients hospitalized in The First Affiliated Hospital of China Medical University from January 2010 to January 2012.There was no repeated strain.The 91 strains consisted of 44 Enterococcus faecalis and 47 Enterococcus faecium strains.Agar dilution method was used to measure the minimum inhibitory concentrations (MIC) of the five antibiotics against the 91 enterococci strains under standard low inoculation concentration (1 × 105 colony-forming units [cfu]/mL) and high inoculation concentration (1 × 107 cfu/mL).Results For all tested strains,MIC of fosfomycin fluctuated between 16-64 tμg/mL in high and low inoculated concentrations.Among the tested strains,both Enterococcus faecalis and Enterococcus faecium had the highest drug-sensitive rate (100.0％) and the lowest drugresistance rate (0) to fosfomycin.With the increase of the inoculation concentration,MIC of the five antibiotics increased less than 8 times,which indicated that there was no inoculation effect.Conclusion Fosfomycin shows high drug-sensitivity against enterococci without inoculum effect in vitro,and it is recommended for the treatment of enterococci-infected patients.
