CAJ | 학술논문

目的研究快速上浮脱险致减压病动物凝血纤溶系统的变化,从而为快速上浮脱险减压病筛选能反映其损伤程度的敏感指标.方法雄性SD大鼠80只,按数字表法随机分为2组,正常对照组(对照组)20只,快速上浮脱险实验组(实验组)60只,根据动物是否死亡进而分为死亡组和存活组.实验组以21/7指数速率加压至1.5 MPa(表压),停留4 min后匀速减压至常压.观察实验组大鼠出舱后30 min内的存活状况.如果大鼠30 min内死亡,则立即抽取其下腔静脉血;如果30 min内未死亡,则在30 min后抽取其下腔静脉血,血标本置于3.5％枸橼酸钾及1％肝素内抗凝,用于检测凝血6项指标和血小板计数.结果实验组大鼠30 min内死亡率30只(50％).对照组、存活组与死亡组大鼠的血小板计数比较差异无统计学意义(P＞0.05);存活组、死亡组及对照组大鼠的凝血酶时间分别为(28.00±1.69)s、(27.21 ±3.82)s与(30.91±2.82)s,组间比较差异均有统计学意义(P＜0.01),对照组大鼠的纤维蛋白原(2.91±0.43)g/L和死亡组(3.72±1.55)g/L比较差异有统计学意义(P＜0.05),存活组大鼠的D-二聚体(0.19±0.05) μg/L和死亡组(0.24±0.08) μg/L比较差异有统计学意义(P＜0.05).结论凝血酶时间、纤维蛋白原和D-二聚体可综合反映快速上浮脱险致减压病的发生以及造成损伤的严重程度,可作为反映损伤程度的指标.
목적 연구쾌속상부탈험치감압병동물응혈섬용계통적변화,종이위쾌속상부탈험감압병사선능반영기손상정도적민감지표.방법 웅성SD대서80지,안수자표법수궤분위2조,정상대조조(대조조)20지,쾌속상부탈험실험조(실험조)60지,근거동물시부사망진이분위사망조화존활조.실험조이21/7지수속솔가압지1.5 MPa(표압),정류4 min후균속감압지상압.관찰실험조대서출창후30 min내적존활상황.여과대서30 min내사망,칙립즉추취기하강정맥혈;여과30 min내미사망,칙재30 min후추취기하강정맥혈,혈표본치우3.5％구연산갑급1％간소내항응,용우검측응혈6항지표화혈소판계수.결과 실험조대서30 min내사망솔30지(50％).대조조、존활조여사망조대서적혈소판계수비교차이무통계학의의(P＞0.05);존활조、사망조급대조조대서적응혈매시간분별위(28.00±1.69)s、(27.21 ±3.82)s여(30.91±2.82)s,조간비교차이균유통계학의의(P＜0.01),대조조대서적섬유단백원(2.91±0.43)g/L화사망조(3.72±1.55)g/L비교차이유통계학의의(P＜0.05),존활조대서적D-이취체(0.19±0.05) μg/L화사망조(0.24±0.08) μg/L비교차이유통계학의의(P＜0.05).결론 응혈매시간、섬유단백원화D-이취체가종합반영쾌속상부탈험치감압병적발생이급조성손상적엄중정도,가작위반영손상정도적지표.
Objective To study changes in coagulation and fibrinolysis system of animals with decompression sickness caused by fast buoyancy ascent escape.Methods Eighty male SD rats were randomly divided into 2 groups:the normal control group (n =20),the fast buoyancy ascent escape group (or simply the experimental group) (n =60) and the experimental group was further divided into the death and survival groups,based on whether the animals died or survived after escape.The animals in the experimental group were compressed to a pressure of 1.5 MPa at an exponential rate of 2t/7 with compressed air,then,after staying for 4 min at thc above pressure,the animals were decompressed to atmospheric pressure at linear speed.The animals that survived within 30 min was observed after they were brought out of the chamber.If the animals died within 30 min,blood samples were immediately collected from inferior vena cava.If death did not occur within 30 min,then blood samples were collected 30 min later.The collected blood samples were placed in 3.5％ potassium citrate or 1％ heparin for anticoagulation,for the detection of coagulation and platelet count.Results The mortality for the animals in the experimental group was 50％.No statistical significance could be seen in platelet counts,when comparisons were made between the 3 groups,but there was statistical significance in coagulation time,when comparisons were made between the 3 groups.There were statistical differences in the lcvels of fibrinogen,when comparisons were made between the normal control group (2.91 ± 0.43) g/L and the death group (3.72 ± 1.55)g/L.And statistical significance could also be noted in the levels of D-dimer,when comparisons were made between the survival group (0.19 ± 0.05) μg/L and the death group (0.24 ± 0.08) μg/L.Conclusions Coagulation time,fibrinogen and D-dimer could be used as indicators for the occurrence of DCS induced by fast buoyancy ascent escape,and seriousness of damage as well.