CAJ | 학술논문

目的:比较HPLC-UV与HPLC-FLED测定大黄药材中5种蒽醌苷元的含量.方法:采用HPLC-UV和HPLC-FLED测定,Venusil XBP-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.1％磷酸溶液(80∶20),流速1.0 mL·min-1,柱温25℃,紫外检测波长254 nm,荧光检测器激发波长435 nm,发射波长515 nm.结果:紫外检测器下芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进样量在14.64～146.40,39.0 ～390.4,36.8 ～368.0,17.0～ 170.4,19.2 ～192.0 ng与其峰面积呈良好线性关系,相关系数分别为0.999 7,0.999 7,0.999 4,0.999 4,0.999 4;荧光检测器下芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进样量在3.7 ～292.8,9.8 ～780.8,9.2～736.0,4.3～340.8,4.8 ～384.0 ng与其峰面积呈良好线性关系,相关系数分别为0.999 4,0.999 5,0.999 4,0.999 7,0.999 6.紫外检测器的检测限(LOD)分别为0.32,0.84,0.61,1.28,2.88 ng,定量限(LOQ)为1.05,2.79,2.04,4.26,9.60 ng;荧光检测器的检测限(LOD)为0.03,0.12,0.18,0.09,0.07 ng,定量限(LOQ)为0.09,0.39,0.61,0.28,0.24 ng.结论:HPLC-荧光检测法比HPLC-紫外检测法方法简单准确,无干扰,更适合大黄药材的含量测定及其质量标准的建立.
목적:비교HPLC-UV여HPLC-FLED측정대황약재중5충은곤감원적함량.방법:채용HPLC-UV화HPLC-FLED측정,Venusil XBP-C18색보주(4.6 mm×250 mm,5μm),류동상갑순-0.1％린산용액(80∶20),류속1.0 mL·min-1,주온25℃,자외검측파장254 nm,형광검측기격발파장435 nm,발사파장515 nm.결과:자외검측기하호회대황소、대황산、대황소、대황분、대황소갑미진양량재14.64～146.40,39.0 ～390.4,36.8 ～368.0,17.0～ 170.4,19.2 ～192.0 ng여기봉면적정량호선성관계,상관계수분별위0.999 7,0.999 7,0.999 4,0.999 4,0.999 4;형광검측기하호회대황소、대황산、대황소、대황분、대황소갑미진양량재3.7 ～292.8,9.8 ～780.8,9.2～736.0,4.3～340.8,4.8 ～384.0 ng여기봉면적정량호선성관계,상관계수분별위0.999 4,0.999 5,0.999 4,0.999 7,0.999 6.자외검측기적검측한(LOD)분별위0.32,0.84,0.61,1.28,2.88 ng,정량한(LOQ)위1.05,2.79,2.04,4.26,9.60 ng;형광검측기적검측한(LOD)위0.03,0.12,0.18,0.09,0.07 ng,정량한(LOQ)위0.09,0.39,0.61,0.28,0.24 ng.결론:HPLC-형광검측법비HPLC-자외검측법방법간단준학,무간우,경괄합대황약재적함량측정급기질량표준적건립.