CAJ | 학술논문

目的：研究高脂饮食喂养诱导的胰岛素抵抗（IR）小鼠骨骼肌中磷脂酰肌醇3激酶（PI3K）表达及肌糖原含量的改变。方法将20只雄性 KM小鼠随机分为对照组（NC 组，10只）和 IR 组（10只），NC 组予常规饲料，IR 组予高脂饲料。16周时 IR 造模成功，记录小鼠体质量、空腹血糖（FBG）和空腹胰岛素（FINS），并计算稳态模型胰岛素抵抗指数（HOMA-IR）；Western blot 法检测骨骼肌 PI3K 的表达，过碘酸雪夫（PAS）染色观察骨骼肌糖原含量的变化。结果与 NC 组比较，IR 组小鼠体质量、FBG、FINS 及 HOMA-IR 增高（P 均＜0．05），IR 组骨骼肌中 PI3K 的表达显著降低（P ＜0．05）；PAS 染色显示 NC 组骨骼肌细胞内紫红色着色广泛，着色颗粒较多，IR 组着色浅，着色颗粒较少。结论骨骼肌 PI3K 表达降低可能是导致肌肉 IR 产生的原因之一。
목적：연구고지음식위양유도적이도소저항（IR）소서골격기중린지선기순3격매（PI3K）표체급기당원함량적개변。방법장20지웅성 KM소서수궤분위대조조（NC 조，10지）화 IR 조（10지），NC 조여상규사료，IR 조여고지사료。16주시 IR 조모성공，기록소서체질량、공복혈당（FBG）화공복이도소（FINS），병계산은태모형이도소저항지수（HOMA-IR）；Western blot 법검측골격기 PI3K 적표체，과전산설부（PAS）염색관찰골격기당원함량적변화。결과여 NC 조비교，IR 조소서체질량、FBG、FINS 급 HOMA-IR 증고（P 균＜0．05），IR 조골격기중 PI3K 적표체현저강저（P ＜0．05）；PAS 염색현시 NC 조골격기세포내자홍색착색엄범，착색과립교다，IR 조착색천，착색과립교소。결론골격기 PI3K 표체강저가능시도치기육 IR 산생적원인지일。
Objective To study the changes in phosphatidylinositol 3-kinase(PI3K)expression and glycogen content in the skeletal muscle of mice with insulin resistance(IR)fed with high fat diet. Methods 20 male KMmice were randomly divided into normal control group(NC group,n =10)and IR group(n =10).The mice in NC group were fed with routine diet and the mice in IR group were fed with high-fat diet.At the 16th week,IR models were tested successful.Record body weight,fasting blood glu-cose(FBG)and fasting insulin(FINS),then calculated Homeostasis Model Assessment for Insulin Resist-ance(HOMA-IR).The expression of PI3K in the skeletal muscle were measured by Western blotting method and the muscle glycogen content were measured by Periodic Acid-Schiff Staln(PAS).Results Compared with NC group,the body weight,FBG,FINS and HOMA-IR of IR group were higher(respective-ly P <0.05),the PI3K in the skeletal muscle of IR group decreased significantly(P <0.05).The skeletal muscle cells in NC control were colored extensively and more colored granules,while IR gruop were col-ored light and less colored granules.Conclusion The reduced expression of PI3K in skeletal muscle may be one of the reasons leading to muscle IR.