CAJ | 학술논문

目的：通过研究神经生长因子（NGF）与血清肾上腺素浓度之间的关系，探讨呼吸道合胞病毒（RSV ）感染相关性哮喘的发病机制，指导临床诊治RSV感染相关性哮喘。方法取健康的SD幼鼠60只随机分为3组，分别为RSV组、抗NGF组及对照组，每组20只，SD幼鼠培养3个月后处死，取肾上腺组织、血清等，取SD大鼠肾上腺组织，利用电子显微镜、试剂盒、免疫组化ELISA法及RT‐PCR检测观察肾上腺髓质细胞的超微结构变化、NGF和肾上腺素浓度及NGF与神经丝蛋白的表达量。结果 SD大鼠肾上腺髓质细胞NGF的表达量RSV组明显高于抗NGF组和对照组、抗 NGF组S明显高于对照组，差异有统计学意义（P＜0．05）；SD大鼠血清中NGF浓度RSV组明显高于对照组和抗NGF组，差异有统计学意义（P＜0．05）；SD大鼠血清中肾上腺素的浓度RSV组和抗NGF组明显低于对照组、RSV组明显弱于抗NGF组，差异有统计学意义（P＜0．05）；SD大鼠肾上腺髓质细胞神经丝mRNA的表达量RSV组和抗NGF组较对照组明显升高、RSV组较抗NGF组SD大鼠明显升高，差异有统计学意义（P＜0．05）。结论 RSV感染相关性哮喘的发病机制可能为其感染后造成肾上腺组织和血清NGF的表达量升高，NGF促进肾上腺髓质细胞向神经元转化，导致血清中肾上腺素浓度降低，不能维持支气管的舒张，从而引起哮喘的发生。
목적：통과연구신경생장인자（NGF）여혈청신상선소농도지간적관계，탐토호흡도합포병독（RSV ）감염상관성효천적발병궤제，지도림상진치RSV감염상관성효천。방법취건강적SD유서60지수궤분위3조，분별위RSV조、항NGF조급대조조，매조20지，SD유서배양3개월후처사，취신상선조직、혈청등，취SD대서신상선조직，이용전자현미경、시제합、면역조화ELISA법급RT‐PCR검측관찰신상선수질세포적초미결구변화、NGF화신상선소농도급NGF여신경사단백적표체량。결과 SD대서신상선수질세포NGF적표체량RSV조명현고우항NGF조화대조조、항 NGF조S명현고우대조조，차이유통계학의의（P＜0．05）；SD대서혈청중NGF농도RSV조명현고우대조조화항NGF조，차이유통계학의의（P＜0．05）；SD대서혈청중신상선소적농도RSV조화항NGF조명현저우대조조、RSV조명현약우항NGF조，차이유통계학의의（P＜0．05）；SD대서신상선수질세포신경사mRNA적표체량RSV조화항NGF조교대조조명현승고、RSV조교항NGF조SD대서명현승고，차이유통계학의의（P＜0．05）。결론 RSV감염상관성효천적발병궤제가능위기감염후조성신상선조직화혈청NGF적표체량승고，NGF촉진신상선수질세포향신경원전화，도치혈청중신상선소농도강저，불능유지지기관적서장，종이인기효천적발생。
OBJECTIVE To study the relationship between the nerve growth factor (NGF) and the concentration of serum adrenaline and explore the pathogenesis of respiratory syncytial virus (RSV) infection‐related asthma so as to provide guidance for clinical diagnosis and treatment of the RSV infection‐related asthma .METHODS A total of 60 healthy SD rats were randomly divided into three groups :the RSV group ,the anti‐NGF group ,and the control group ,with 20 cases in each .The SD rats were killed after the culture for three months ,then the adrenal tissue and serum were collected;the change of the ultramicro‐structure of adrenal medullary cells was observed by using electron microscope ,the concentration of NGF was determined with the use of ELISA ,the concentration of serum adrenaline was tested ,and the expression of NF‐L mRNA was determined by means of RT‐PCR .RESULTS The NGF expression of the RSV group was significantly higher than that of the anti‐NGF group and the control group , and the NGF expression of the anti‐NGF group was significantly higher than that of the control group(P<0 .05) . The concentration of serum NGF of the RSV group was significantly higher than that of the control group and the anti‐NGF group(P<0 .05) .the concentration of serum adrenaline of the RSV group and the anti‐NGF group was significantly lower than that of the control group ,while the concentration of serum adrenaline of the RSV group was significantly lower than that of the anti‐NGF group(P<0 .05) .The expression volume of medullary cells NF‐L mRNA of the RSV group and the anti‐NGF group were significantly higher than that of the control group ,while the expression volume of medullary cells NF‐L mRNA of the RSV group was significantly higher than that of the anti‐NGF group(P<0 .05) .CONCLUSION The pathogenesis of the RSV infection‐related asthma may lead to the elevated expression volume of adrenal tissue and serum NGF ,the NGF promotes the transformation of adrenal me‐dullary cells into neurons ,which results in the decrease of the concentration of serum adrenaline ,incapable of maintaining the bronchial dilation ,and therefore leads to the asthma .