CAJ | 학술논문

旨在对烟草Ⅰ型几丁质酶进行生物信息学分析，了解其生物学特征，为下一步研究烟草Ⅰ型几丁质酶在烟草抗病过程中的作用奠定基础。采用生物信息学的方法，对已在 NCBI上登陆的烟草、拟南芥、水稻、菜豆的Ⅰ型几丁质酶（chitinase，ChiⅠ）氨基酸序列特征和进化关系进行了预测和分析。结果表明，这4种植物Ⅰ型几丁质酶的分子量大小在28~37 kDa之间，均具有信号肽，结构域高度保守，均具有几丁质结合区（ Cht BD1）和催化功能区（Glyco_hydro_19）；烟草中的3种Ⅰ型几丁质酶序列相似性较高，可以聚为一类；烟草与拟南芥和菜豆中的一种 ChiⅠ遗传距离较近，而与水稻中 ChiⅠ的遗传距离较远。通过分析发现，烟草Ⅰ型几丁质酶有着与其他植物中该酶相同的特性，为进一步研究烟草抗病蛋白提供理论依据。
지재대연초Ⅰ형궤정질매진행생물신식학분석，료해기생물학특정，위하일보연구연초Ⅰ형궤정질매재연초항병과정중적작용전정기출。채용생물신식학적방법，대이재 NCBI상등륙적연초、의남개、수도、채두적Ⅰ형궤정질매（chitinase，ChiⅠ）안기산서렬특정화진화관계진행료예측화분석。결과표명，저4충식물Ⅰ형궤정질매적분자량대소재28~37 kDa지간，균구유신호태，결구역고도보수，균구유궤정질결합구（ Cht BD1）화최화공능구（Glyco_hydro_19）；연초중적3충Ⅰ형궤정질매서렬상사성교고，가이취위일류；연초여의남개화채두중적일충 ChiⅠ유전거리교근，이여수도중 ChiⅠ적유전거리교원。통과분석발현，연초Ⅰ형궤정질매유착여기타식물중해매상동적특성，위진일보연구연초항병단백제공이론의거。
This study almed to analyze the characteristics of Chi I (cIass I chitinase) in tobacco by using bioinformatics methods and tooIs so as to Iay a foundation for further study on disease resistance of Chi I in tobacco. The amino acid sequence characteristics and phyIogenetic reIationships of Chi Is of Nicotiana tabacum, Ara-bidopsis thaliana, Oryza sativa and Phaseolus vulgaris from NCBI were predicted and analyzed by using bioinformatics methods. The resuIts showed that the moIec-uIar weights of Chi Is with signal peptides ranged from 28 to 37 kDa. The se-quences of Chi Is, which al contalned both N-terminal chitin binding domaln (Chit BD1)andcatalyticdomaln(CIyco hydro-19),werehighIyconserved.Thesequences of Nt-ChiA, Nt-ChiB and Nt-ChiC, three Chi Is from Nicotiana tabacum had high simiIarity, so their phyIogenetic reIationships were also cIose. The reIationships be-tween Chi Is from Nicotiana tabacum and one of the Chi Is from Arabidopsis thaliana and Phaseolus vulgaris were cIoser than those between Chi Is from Nico-tiana tabacum and Oryza sativa. The resuIts indicated that the properties of Chi I in tobacco were almost the same as those in other pIants. This study wiI provide a basis for further researches on disease resistance proteins in tobacco.