CAJ | 학술논문

为研究硝态氮含量对调制后烟叶高温贮藏过程中TSNA形成的影响，设计白肋烟田间施氮肥量试验、室内对烤烟烟叶添加不同浓度硝酸盐试验，并对白肋烟和烤烟的叶片和烟梗分别取样，得到硝态氮含量差异较大样品。将样品在高温（45℃）条件下贮藏15 d，以在低温（10℃）条件下贮藏作为对照，测定所有样品的硝态氮和TSNA含量。结果表明：烟叶中硝态氮含量与施氮量呈正相关；新调制的烟叶硝态氮含量与TSNAs相关性较小，在高温贮藏15 d后，其相关性显著增加，烟叶硝态氮含量与高温贮藏期间TSNAs的增加量呈显著正相关；白肋烟和烤烟均表现出烟叶主脉中的硝态氮含量远高于叶片，高温贮藏15 d后，主脉TSNA增加量显著高于叶片；在人工添加硝酸盐的试验中，随添加硝酸盐量的增加，烤烟烟叶硝态氮含量及高温贮藏后TSNA含量显著增加。以上说明硝态氮含量与高温贮藏过程中TSNAs的形成有密切关系，通过农业措施降低烟叶硝态氮积累是减少贮藏过程中TSNA形成的有效途径。
위연구초태담함량대조제후연협고온저장과정중TSNA형성적영향，설계백륵연전간시담비량시험、실내대고연연협첨가불동농도초산염시험，병대백륵연화고연적협편화연경분별취양，득도초태담함량차이교대양품。장양품재고온（45℃）조건하저장15 d，이재저온（10℃）조건하저장작위대조，측정소유양품적초태담화TSNA함량。결과표명：연협중초태담함량여시담량정정상관；신조제적연협초태담함량여TSNAs상관성교소，재고온저장15 d후，기상관성현저증가，연협초태담함량여고온저장기간TSNAs적증가량정현저정상관；백륵연화고연균표현출연협주맥중적초태담함량원고우협편，고온저장15 d후，주맥TSNA증가량현저고우협편；재인공첨가초산염적시험중，수첨가초산염량적증가，고연연협초태담함량급고온저장후TSNA함량현저증가。이상설명초태담함량여고온저장과정중TSNAs적형성유밀절관계，통과농업조시강저연협초태담적루시감소저장과정중TSNA형성적유효도경。
Field experiment was conducted to determine the effect of nitrate nitrogen content on TSNAs formation during high temperature storage. Different concentration of nitrate was applied in tobacco growing season. Leaf samples from lamina and midrib of both burley and flue-cured tobacco were collected. Lab experiment was conducted in a temperature-controlled chamber at 45℃ and 10℃ for 15d. Nitrate nitrogen and TSNA contents of all samples were determined. Results indicated that nitrate nitrogen content increased with the increment of nitrogen fertilizer. Content of nitrate nitrogen and TNSA had a low correlation in newly cured tobacco. However, after high temperature storage for 15d, the correlation significantly increased. Nitrate nitrogen content and TSNAs increment during high temperature storage showed a significant positive correlation. Nitrate nitrogen content in midrib was much higher than that in lamina in both burley and flue-cured tobacco. After high temperature storage for 15d, TSNAs increment in midrib was significantly higher than that in lamina. With the increase of nitrate applied to flue-cured tobacco leaves, nitrate nitrogen and TSNA content after high temperature storage increased significantly. These results indicated that nitrate nitrogen content was closely related to the formation of TSNAs during high temperature storage. Therefore, reduction of leaf nitrate content through agronomic measures is effective in reducing TSNA formation during storage.