东北电力大学学报
東北電力大學學報
동북전력대학학보
JOURNAL OF NORTHEAST DIANLI UNIVERSITY
2015年
2期
57-60
,共4页
蛋白%吸附%脱附%配位%金纳米粒子
蛋白%吸附%脫附%配位%金納米粒子
단백%흡부%탈부%배위%금납미입자
Protein%Adsorption%Desorption%Coordination%Au nanoparticles
利用固定金属离子亲和色谱法即IMAC ( Immobilized Metal-ion affinity chromatography)的原理，使用表面修饰Ni2+离子的金纳米粒子对牛血红蛋白的吸附和脱附行为进行了研究。粒子对牛血红蛋白的最大吸附量可达到5.56mg/mg。粒子可以将牛血红蛋白从牛血红蛋白和牛血清蛋白混合物中分离出来。通过紫外光谱及电泳测试进一步论证了前面的结论。这种方法简单、有效、方便，在牛血红蛋白的分离纯化方面有着很好的应用前景。
利用固定金屬離子親和色譜法即IMAC ( Immobilized Metal-ion affinity chromatography)的原理，使用錶麵脩飾Ni2+離子的金納米粒子對牛血紅蛋白的吸附和脫附行為進行瞭研究。粒子對牛血紅蛋白的最大吸附量可達到5.56mg/mg。粒子可以將牛血紅蛋白從牛血紅蛋白和牛血清蛋白混閤物中分離齣來。通過紫外光譜及電泳測試進一步論證瞭前麵的結論。這種方法簡單、有效、方便，在牛血紅蛋白的分離純化方麵有著很好的應用前景。
이용고정금속리자친화색보법즉IMAC ( Immobilized Metal-ion affinity chromatography)적원리，사용표면수식Ni2+리자적금납미입자대우혈홍단백적흡부화탈부행위진행료연구。입자대우혈홍단백적최대흡부량가체도5.56mg/mg。입자가이장우혈홍단백종우혈홍단백화우혈청단백혼합물중분리출래。통과자외광보급전영측시진일보론증료전면적결론。저충방법간단、유효、방편，재우혈홍단백적분리순화방면유착흔호적응용전경。
The adsorption behavior of bovine hemoglobin on Ni2+immobilized gold nanorparticles was investiga-ted,which refer to the method of Immobilized Metal-ion affinity chromatography ( IMAC) . The particles showed high absorption efficiency to bovine hemoglobin and the maximum extraction amounts reached 5. 56mg/mg. The particles were applied to separate bovine hemoglobin from the mixture of bovine hemoglobin and bovine serum albumin. These results were proved by UV-vis spectra and Protein Gel Electrophoresis. This method is a sim-ple,convenient and effective way,which has potential application in removing abundant protein in proteomic a-nalysis.
