CAJ | 학술논문

[目的]采用 ISSR分子标记对苦瓜杂交一代品种秀玉1号纯度进行鉴定，为生产上提高苦瓜新品种纯度鉴定效率提供参考。[方法]利用ISSR技术和91条引物，对苦瓜品种秀玉1号及其亲本的DNA指纹进行分析。[结果]从91条引物中筛选出两条特征引物能同时扩增出F1代及其父母本的多态性条带，分别是ISSR-845和 ISSR-891，两个特异位点分别位于510和300 bp处。其中ISSR-891能够准确区分母本自交系与杂种 F1，经群体验证表明该标记与田间鉴定结果高度一致。[结论] ISSR技术具有准确、简便、高效和实用强等优点，可用于生产上苦瓜种子纯度的快速鉴定。
[목적]채용 ISSR분자표기대고과잡교일대품충수옥1호순도진행감정，위생산상제고고과신품충순도감정효솔제공삼고。[방법]이용ISSR기술화91조인물，대고과품충수옥1호급기친본적DNA지문진행분석。[결과]종91조인물중사선출량조특정인물능동시확증출F1대급기부모본적다태성조대，분별시ISSR-845화 ISSR-891，량개특이위점분별위우510화300 bp처。기중ISSR-891능구준학구분모본자교계여잡충 F1，경군체험증표명해표기여전간감정결과고도일치。[결론] ISSR기술구유준학、간편、고효화실용강등우점，가용우생산상고과충자순도적쾌속감정。
Objective] This study was conducted to verify the feasibiIity of ISSR marker for identifying genetic purity of bitter gourd hybrid, and thus to provide an effective method for seed purity test in production practices. [Method] The DNA fin-gerprints of a bitter gourd cuItivar Xiuyu 1 and its parents were analyzed using IS-SR marker with 91 primers. [Result] Two primers ISSR-845 and ISSR-891 which ampIified two DNA bands of 510 and 300 bp respectiveIy from F1 generation and its parents were screened out from 91 primers. ISSR-845 couId distinguish the male parent from F1 hybrid and the female parent, whiIe ISSR-891 couId distinguish the female parent inbred Iine from Xiuyu 1. Seed purity test with the specific markers gave the same resuIt with fiIed trials based on morphoIogical identification. [Conclu-sion] ISSR marker is an accurate, simpIe and effective method for seed purity test bitter gourd hybrid, and thus can be used in production practices.