CAJ | 학술논문

利用匀强电场对杉木当年生茎段外植体进行处理，研究电场强度和处理时间对杉木茎段芽诱导的影响。在1．0 k·Vcm －1的电场强度下处理5 min，芽诱导率最高，达100％，比对照组增加62．50％；芽形成能力达835．00％，是对照组的2．78倍，亦高于其它处理组合；平均芽数最高达到12．50个，是对照组的2．50倍，与对照组具有显著差异。7．0 kV· cm －1电场强度处理1 min后，随着处理时间的增加，诱导率均为0，明显抑制了杉木芽的分化；处理3 min后，外植体存活率均呈下降趋势，致死作用增大。结果表明：电场强度和处理时间对杉木外植体的芽诱导率和形成能力具显著影响。
이용균강전장대삼목당년생경단외식체진행처리，연구전장강도화처리시간대삼목경단아유도적영향。재1．0 k·Vcm －1적전장강도하처리5 min，아유도솔최고，체100％，비대조조증가62．50％；아형성능력체835．00％，시대조조적2．78배，역고우기타처리조합；평균아수최고체도12．50개，시대조조적2．50배，여대조조구유현저차이。7．0 kV· cm －1전장강도처리1 min후，수착처리시간적증가，유도솔균위0，명현억제료삼목아적분화；처리3 min후，외식체존활솔균정하강추세，치사작용증대。결과표명：전장강도화처리시간대삼목외식체적아유도솔화형성능력구현저영향。
Chinese fir seedling stem section of explants was treated by the uniform electric field , and the impact of different electric field intensity and different processing time on bud induction of Chinese fir stem section was investigated .Bud induction rate was 100%under the electric field intensity as 1.0 kV· cm-1 for 5 min, increasing 62.50 percent compared with the control group .The capacity of bud formation was 2.78 times that of the control group , up to 835.00%, also higher than other treatment combinations . The average number of buds reached the highest as 12.50, 2.50 times as that of the control group .Under the electric field intensity as 7.0 kV· cm-1 for 1 min, with the increase of processing time , bud induction rate was 0, and the inhibitory effect on bud differentiation was remarkable , after 3 min, the survival rate of explants were decreased , and lethal effect on explants was increased.The results indicated that the bud induction rate and forming ability of Chinese fir explants were affected by electric field intensity and the processing time significantly .