CAJ | 학술논문

为将白僵菌应用于烟叶储存醇化的仓储环境，对其进行抗干旱、耐高温菌株选育。通过温度筛选获得能够在35℃下生长的球孢白僵菌GZUIFR-GL5菌株。该菌株通过紫外线和微波在150s下的组合诱变和PEG胁迫筛选，得到具有生长稳定且能够耐受-0.8Mpa的较低水势和35℃环境条件的突变菌株GZUIFR-GL5-08i。测定GZUIFR-GL5-08i部分生理指标发现蛋白含量、脯氨酸、SOD、CAT、POD等含量均比诱变前菌株提高，反映了在抗旱机理方面虫生真菌与植物抗逆机理有相似性。
위장백강균응용우연협저존순화적창저배경，대기진행항간한、내고온균주선육。통과온도사선획득능구재35℃하생장적구포백강균GZUIFR-GL5균주。해균주통과자외선화미파재150s하적조합유변화PEG협박사선，득도구유생장은정차능구내수-0.8Mpa적교저수세화35℃배경조건적돌변균주GZUIFR-GL5-08i。측정GZUIFR-GL5-08i부분생리지표발현단백함량、포안산、SOD、CAT、POD등함량균비유변전균주제고，반영료재항한궤리방면충생진균여식물항역궤리유상사성。
Screening and breeding of drought-resistant and high temperature-resistantBeauveria bassiana was conducted so as to make it adaptable in leaf tobacco warehouse. GZUIFR-GL5-08i stralns were screened out through a combined ultraviolet and microwave mutagenic experiment. Results showed that GZUIFR-GL5-08i sustalned stable growth on the condition of water potential 0.8Mpa and temperature 35℃. Content of protein, proiline, SOD, CAT, POD were higher than that before mutation, which showed similarity in draught-resistance mechanism between entomogenous fungi and plants.