森林与环境学报
森林與環境學報
삼림여배경학보
Journal of Forest and Environment
2015年
1期
38-44
,共7页
吴耀军%常明山%黎贵倾%张照远%盛双%梁忠云
吳耀軍%常明山%黎貴傾%張照遠%盛雙%樑忠雲
오요군%상명산%려귀경%장조원%성쌍%량충운
桉叶挥发物%桉树枝瘿姬小蜂%诱导%四臂嗅觉仪%诱集作用
桉葉揮髮物%桉樹枝癭姬小蜂%誘導%四臂嗅覺儀%誘集作用
안협휘발물%안수지영희소봉%유도%사비후각의%유집작용
Eucalyptus volatiles%Leptocybe invasa Fisher&La Salle%induce%four-armed olfactometer%temptation
采用GC-MS和四臂嗅觉仪测定了桉树DH32-28、DH201-2、GL-UG9无性系健康树叶和经桉树枝瘿姬小蜂诱导桉叶挥发物的变化,及其对枝瘿姬小蜂成虫的诱集作用。结果表明,诱导的和健康的桉叶挥发物提取率由高到低依次为DH32-28、DH201-2、GL-UG9无性系,同一无性系诱导的桉叶提取率较健康的低;经诱导的桉叶挥发物萜类和醇类物质种类较健康桉叶的多,绿叶类、醛和酮类、芳香类化合物也较多;在四臂嗅觉仪的终选区,健康的DH201-2和GL-UG9桉叶对桉树枝瘿姬小蜂成虫具有明显的诱集作用,而健康的DH32-28桉叶未表现出明显的诱集作用,经诱导的DH201-2、GL-UG9和DH32-28桉叶挥发物则表现出明显的诱集作用。
採用GC-MS和四臂嗅覺儀測定瞭桉樹DH32-28、DH201-2、GL-UG9無性繫健康樹葉和經桉樹枝癭姬小蜂誘導桉葉揮髮物的變化,及其對枝癭姬小蜂成蟲的誘集作用。結果錶明,誘導的和健康的桉葉揮髮物提取率由高到低依次為DH32-28、DH201-2、GL-UG9無性繫,同一無性繫誘導的桉葉提取率較健康的低;經誘導的桉葉揮髮物萜類和醇類物質種類較健康桉葉的多,綠葉類、醛和酮類、芳香類化閤物也較多;在四臂嗅覺儀的終選區,健康的DH201-2和GL-UG9桉葉對桉樹枝癭姬小蜂成蟲具有明顯的誘集作用,而健康的DH32-28桉葉未錶現齣明顯的誘集作用,經誘導的DH201-2、GL-UG9和DH32-28桉葉揮髮物則錶現齣明顯的誘集作用。
채용GC-MS화사비후각의측정료안수DH32-28、DH201-2、GL-UG9무성계건강수협화경안수지영희소봉유도안협휘발물적변화,급기대지영희소봉성충적유집작용。결과표명,유도적화건강적안협휘발물제취솔유고도저의차위DH32-28、DH201-2、GL-UG9무성계,동일무성계유도적안협제취솔교건강적저;경유도적안협휘발물첩류화순류물질충류교건강안협적다,록협류、철화동류、방향류화합물야교다;재사비후각의적종선구,건강적DH201-2화GL-UG9안협대안수지영희소봉성충구유명현적유집작용,이건강적DH32-28안협미표현출명현적유집작용,경유도적DH201-2、GL-UG9화DH32-28안협휘발물칙표현출명현적유집작용。
GC-MS and four-armed olfactmeter were employed to test the changes of volatiles in healthy leaves of Eucalyptus and induced by Leptocybe invasa Fisher & La Salle in the clones such as DH32-28, DH201-2 and GL-UG9, and to investigate their temptation to the adults .The extraction ratios of induced and healthy volatiles were in the following order :DH32-28>DH201-2>GL-UG9, and the extraction ratio of induced volatiles was lower than that of volatiles from the healthy trees in the same clone .In the induced volatiles, terpene, alcohols, aldehyde, ketone and aromatics were more than those in the healthy leaves .The leaves of healthy DH201-2 and GL-UG9 had remarkable temptation to the adults of Leptocybe invasa Fisher & La Salle, but the leaves of healthy DH32-28 performed insignificantly .Meanwhile the induced volatiles in the leaves of the clones DH 201-2, GL-UG9 and DH32-28 performed obvious temptation .