CAJ | 학술논문

以同期生长的梁山慈竹体细胞突变体No．29植株和实生植株（对照）为材料，对其茎秆化学成分和光合生理相关指标进行测定，并基于RNA高通量转录组测序结果，对No．29植株的纤维素、木质素等合成有关的基因差异表达进行分析。结果表明，与实生植株相比，No．29植株具有较高的株高、胸径、茎秆的鲜重和干重以及纤维素含量、纤维长度和宽度；纤维素含量比实生植株高12．89％；外方和内方纤维股明显增大；灰分含量、卷曲指数和扭结指数明显降低；但发笋量、木质素含量和细小纤维含量没有明显差异。 No．29植株中的CesA、SuSy、UDPase基因分别比实生植株上调了117％、205％、115％，转录因子MYB基因表达上调了214％，驱动蛋白基因表达上调了10倍，动力蛋白基因表达上调了760％。 No．29植株纤维素含量与纤维形态上明显的改变有其遗传基础，为No．29的进一步研究与利用奠定了基础。
이동기생장적량산자죽체세포돌변체No．29식주화실생식주（대조）위재료，대기경간화학성분화광합생리상관지표진행측정，병기우RNA고통량전록조측서결과，대No．29식주적섬유소、목질소등합성유관적기인차이표체진행분석。결과표명，여실생식주상비，No．29식주구유교고적주고、흉경、경간적선중화간중이급섬유소함량、섬유장도화관도；섬유소함량비실생식주고12．89％；외방화내방섬유고명현증대；회분함량、권곡지수화뉴결지수명현강저；단발순량、목질소함량화세소섬유함량몰유명현차이。 No．29식주중적CesA、SuSy、UDPase기인분별비실생식주상조료117％、205％、115％，전록인자MYB기인표체상조료214％，구동단백기인표체상조료10배，동력단백기인표체상조료760％。 No．29식주섬유소함량여섬유형태상명현적개변유기유전기출，위No．29적진일보연구여이용전정료기출。
The chemical components of stem and photosynthetic physiological indexes in the somatic mutant No .29 and the plant of seeds ( CK) were determined .The different expressed genes involved in the biosynthesis of cellulose and lignin from No .29 were analyzed based on RNA high-throughput sequencing , compared with those of CK .The results showed that , compared with the plant of seeds, the significant plant height, diameter at breast height, fresh weight and dry weight of stem, cellulose content, length and width of fiber were found in the mutant No.29.The cellulose content in the mutant No.29 increased 12.89%, compared with CK. The fibre strand outside the central strand and fibre strand inside the central strand and in the mutant No .29 were obviously higher than those of CK .The ash content , curl index and kink index were significantly lower than those of CK .The shooting number , lignin content and fiber fines content were insignificant , compared with CK .The expression of gene of CesA, SuSy, UDPase, transcriptional factor MYB in the mutant No .29 was up regulated by 117%, 205%, 115%, 214%, respectively .The kinesin was up regulated by 10 fold while the dynein was up regulated by 760%.The results indicated that the significant changes of cellulose content and fiber morphology have the genetic bases in the mutant No .29.It laid a foundation for further research and utilization of No .29.