海洋科学
海洋科學
해양과학
MARINE SCIENCES
2015年
3期
26-32
,共7页
金水华%吴宁%刘少芳%江波%梁惠%史大永
金水華%吳寧%劉少芳%江波%樑惠%史大永
금수화%오저%류소방%강파%량혜%사대영
海普诺%胰岛素抵抗%HepG2细胞
海普諾%胰島素牴抗%HepG2細胞
해보낙%이도소저항%HepG2세포
HPN%insulin resistance%HepG2 cell
通过细胞形态观察和CCK-8法研究HPN对HepG2细胞活力的影响。采用高糖高浓度胰岛素持续作用诱导 HepG2细胞建立胰岛素抵抗细胞模型，用多功能自动生化仪葡萄糖-己糖激酶法检测海普诺(HPN)对正常HepG2细胞及胰岛素抵抗HepG2细胞葡萄糖消耗量的影响。实验结果显示，低浓度的HPN对HepG2细胞增殖没有影响。HPN与胰岛素协同作用HepG2细胞，低浓度胰岛素对葡萄糖利用有一定的促进作用；当胰岛素浓度为1×10–6 mol/L时， HPN可明显促进HepG2细胞葡萄糖消耗。进一步研究表明， HPN在5×10–8~1×10–7 mol/L浓度范围内可明显促进胰岛素抵抗HepG2细胞的葡萄糖的消耗。HPN可明显改善HepG2细胞胰岛素抵抗。
通過細胞形態觀察和CCK-8法研究HPN對HepG2細胞活力的影響。採用高糖高濃度胰島素持續作用誘導 HepG2細胞建立胰島素牴抗細胞模型，用多功能自動生化儀葡萄糖-己糖激酶法檢測海普諾(HPN)對正常HepG2細胞及胰島素牴抗HepG2細胞葡萄糖消耗量的影響。實驗結果顯示，低濃度的HPN對HepG2細胞增殖沒有影響。HPN與胰島素協同作用HepG2細胞，低濃度胰島素對葡萄糖利用有一定的促進作用；噹胰島素濃度為1×10–6 mol/L時， HPN可明顯促進HepG2細胞葡萄糖消耗。進一步研究錶明， HPN在5×10–8~1×10–7 mol/L濃度範圍內可明顯促進胰島素牴抗HepG2細胞的葡萄糖的消耗。HPN可明顯改善HepG2細胞胰島素牴抗。
통과세포형태관찰화CCK-8법연구HPN대HepG2세포활력적영향。채용고당고농도이도소지속작용유도 HepG2세포건립이도소저항세포모형，용다공능자동생화의포도당-기당격매법검측해보낙(HPN)대정상HepG2세포급이도소저항HepG2세포포도당소모량적영향。실험결과현시，저농도적HPN대HepG2세포증식몰유영향。HPN여이도소협동작용HepG2세포，저농도이도소대포도당이용유일정적촉진작용；당이도소농도위1×10–6 mol/L시， HPN가명현촉진HepG2세포포도당소모。진일보연구표명， HPN재5×10–8~1×10–7 mol/L농도범위내가명현촉진이도소저항HepG2세포적포도당적소모。HPN가명현개선HepG2세포이도소저항。
The effect of HPN on viability of HepG2 cells was determined by cell morphology and CCK8 assay. In-sulin resistant HepG2 cell model was established by continuously treating cells with high concentrations of glucose and insulin. The glucose consumption was determined by multi-functional automatic glucose-hexokinase method. The results showed that HPN had no effect on proliferation of HepG2 cells at low concentrations., The glucose consumption of HepG2 cells was improved by HPN when collaborating with a low concentration of insulin. When the insulin concentration increased to 1×10–6 mol/L, the HPN can markedly increase the glucose consumption of HepG2 cells.HPN can greatly enhance the glucose consumption in the insulin resistant cells with a concentration range of 5×10–8 mol/L to 1×10–7 mol/L. Therefore, HPN can significantly improve insulin resistance.
