农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2015年
4期
806-809,825
,共5页
油茶籽壳%康宁木霉%羧甲基纤维素酶%响应面法
油茶籽殼%康寧木黴%羧甲基纖維素酶%響應麵法
유다자각%강저목매%최갑기섬유소매%향응면법
CameI la seed sheI%Trichoderma koningi%CarboxymethyI ceI ulase%Re-sponse surface methodology
[目的]研究康宁木霉利用油茶籽壳发酵产纤维素酶的发酵条件。[方法]以羧甲基纤维素酶(Carboxymethyl cellulase, CMCase)活力作为响应值，采用响应面法对发酵条件进行优化在油茶籽壳预处理方法、氮源、起始 pH、发酵时间、接种量、营养液体积对 CMCase酶活力单因素试验的基础上筛选出主要影响因素培养时间、起始 pH和营养液体积进行正交试验。通过Box-Behnken设计，利用 Design Expert软件进行回归分析，得出3种因素的交互作用及最佳发酵条件。[结果]用碱法处理油茶籽壳较为适宜；油茶籽壳发酵产纤维素酶的适宜氮源为2%的（NH4）2SO4；其他适宜条件为接种量5%、初始 pH 5.8、营养液体积22 ml发酵时间5 d。在此条件下， CMCase 酶活达179.15 U/ml，比单因素试验最高酶活力提高了24.52%。[结论]该研究可为油茶籽壳的利用与纤维素酶的生产提供参考。
[目的]研究康寧木黴利用油茶籽殼髮酵產纖維素酶的髮酵條件。[方法]以羧甲基纖維素酶(Carboxymethyl cellulase, CMCase)活力作為響應值，採用響應麵法對髮酵條件進行優化在油茶籽殼預處理方法、氮源、起始 pH、髮酵時間、接種量、營養液體積對 CMCase酶活力單因素試驗的基礎上篩選齣主要影響因素培養時間、起始 pH和營養液體積進行正交試驗。通過Box-Behnken設計，利用 Design Expert軟件進行迴歸分析，得齣3種因素的交互作用及最佳髮酵條件。[結果]用堿法處理油茶籽殼較為適宜；油茶籽殼髮酵產纖維素酶的適宜氮源為2%的（NH4）2SO4；其他適宜條件為接種量5%、初始 pH 5.8、營養液體積22 ml髮酵時間5 d。在此條件下， CMCase 酶活達179.15 U/ml，比單因素試驗最高酶活力提高瞭24.52%。[結論]該研究可為油茶籽殼的利用與纖維素酶的生產提供參攷。
[목적]연구강저목매이용유다자각발효산섬유소매적발효조건。[방법]이최갑기섬유소매(Carboxymethyl cellulase, CMCase)활력작위향응치，채용향응면법대발효조건진행우화재유다자각예처리방법、담원、기시 pH、발효시간、접충량、영양액체적대 CMCase매활력단인소시험적기출상사선출주요영향인소배양시간、기시 pH화영양액체적진행정교시험。통과Box-Behnken설계，이용 Design Expert연건진행회귀분석，득출3충인소적교호작용급최가발효조건。[결과]용감법처리유다자각교위괄의；유다자각발효산섬유소매적괄의담원위2%적（NH4）2SO4；기타괄의조건위접충량5%、초시 pH 5.8、영양액체적22 ml발효시간5 d。재차조건하， CMCase 매활체179.15 U/ml，비단인소시험최고매활력제고료24.52%。[결론]해연구가위유다자각적이용여섬유소매적생산제공삼고。
Objective] This study was conducted to optimize cameI ia seed sheI fer-mentation conditions for ceI uIase production by Trichoderma koningi using response surface methodoIogy. [Method] Fermentation conditions for ceI uIase production from Trichoderma koningi were optimized with response surface method (RSM) by taking carboxymethyI ceI uIase (CMCase) activity as a response indicator. Three factors that affecting CMCase activity were screened out using singIe factor test among pretreatment methods of raw material, nitrogen sources, initial pH values, inocuIum voIume, fermentation time and voIume of Iiquid medium, they were fermentation time, initial pH value, and voIume of Iiquid medium. The optimum conditions of fer-mentation and interaction of the three factors were determined through Box-Behnken design and regression analysis using Design-Expert software. [Result] Pretreatment of cameI ia seed sheI with alkaline was most conducive to CMCase production. The use of 0.2% (NH4)2SO4 as nitrogen source, inocuIum size of 5%, initial pH value of 5.8 and voIume of Iiquid medium at 22 mI were the best fermentation conditions for maximizing CMCase production by T. koningi from cameI ia seed sheI . Under these conditions, 179.15 U/mI of CMCase was obtalned after 5 d of fermentation, which was improved by 24.52% compared with the maximum CMCase activity of singIe factor test. [Conclusion] The resuIts wiI provide some references for use of cameI ia seed sheI and ceI uIase production.
