内蒙古医科大学学报
內矇古醫科大學學報
내몽고의과대학학보
Journal of Inner Mongolia Medical University
2015年
1期
34-39
,共6页
肝移植%单纯冷藏%低温机械灌注%免疫%不良反应
肝移植%單純冷藏%低溫機械灌註%免疫%不良反應
간이식%단순랭장%저온궤계관주%면역%불량반응
liver transplantation%static cold storage%hypothermic machine perfusion%CD68%CRP%adverse effect
目的：：考察单纯冷藏与低温机械灌注保存对肝移植前后CD 68、C反应蛋白( C-Reactive protein, CRP)、Fibulin-1、不良反应和存活情况的影响。方法：将犬随机分为单纯冷冻保存组20只和低温机械灌注保存组20只,单纯冷冻保存组取出的肝移植给低温机械灌注保存组,低温机械灌注保存组取出的肝移植给单纯冷冻保存组。结果：取肝时,两个组别的CD 68和CRP无显著差异(P>0.05)；移植前,两个组别的CD 68和Fibulin-1无显著差异(P>0.05),与单纯冷冻保存组比,低温机械灌注保存组移植前C-反应蛋白和Fibulin-1显著降低(P<0.01),低温机械灌注保存组移植60 min,CD 68,CRP和Fibulin-1显著降低(P<0.01)；两个组别取肝的肝病理切片正常,肝移植60 min,单纯冷冻保存组的肝脏出现较大面积的嗜中性粒细胞浸润,大面积坏死,低温机械灌注保存组正常。单纯冷冻保存组和低温机械灌注保存组术后6 h内死亡、存活≥5 d的犬例数无显著差异(P>0.05),与单纯冷冻保存组比,术后3 d内出现死亡例数显著降低(P<0.05),死亡犬均伴有不同程度的黄疸。结论：低温机械灌注保存可显著降低CD 68和CRP,改善病理坏死,提高成活率。
目的：：攷察單純冷藏與低溫機械灌註保存對肝移植前後CD 68、C反應蛋白( C-Reactive protein, CRP)、Fibulin-1、不良反應和存活情況的影響。方法：將犬隨機分為單純冷凍保存組20隻和低溫機械灌註保存組20隻,單純冷凍保存組取齣的肝移植給低溫機械灌註保存組,低溫機械灌註保存組取齣的肝移植給單純冷凍保存組。結果：取肝時,兩箇組彆的CD 68和CRP無顯著差異(P>0.05)；移植前,兩箇組彆的CD 68和Fibulin-1無顯著差異(P>0.05),與單純冷凍保存組比,低溫機械灌註保存組移植前C-反應蛋白和Fibulin-1顯著降低(P<0.01),低溫機械灌註保存組移植60 min,CD 68,CRP和Fibulin-1顯著降低(P<0.01)；兩箇組彆取肝的肝病理切片正常,肝移植60 min,單純冷凍保存組的肝髒齣現較大麵積的嗜中性粒細胞浸潤,大麵積壞死,低溫機械灌註保存組正常。單純冷凍保存組和低溫機械灌註保存組術後6 h內死亡、存活≥5 d的犬例數無顯著差異(P>0.05),與單純冷凍保存組比,術後3 d內齣現死亡例數顯著降低(P<0.05),死亡犬均伴有不同程度的黃疸。結論：低溫機械灌註保存可顯著降低CD 68和CRP,改善病理壞死,提高成活率。
목적：：고찰단순랭장여저온궤계관주보존대간이식전후CD 68、C반응단백( C-Reactive protein, CRP)、Fibulin-1、불량반응화존활정황적영향。방법：장견수궤분위단순냉동보존조20지화저온궤계관주보존조20지,단순냉동보존조취출적간이식급저온궤계관주보존조,저온궤계관주보존조취출적간이식급단순냉동보존조。결과：취간시,량개조별적CD 68화CRP무현저차이(P>0.05)；이식전,량개조별적CD 68화Fibulin-1무현저차이(P>0.05),여단순냉동보존조비,저온궤계관주보존조이식전C-반응단백화Fibulin-1현저강저(P<0.01),저온궤계관주보존조이식60 min,CD 68,CRP화Fibulin-1현저강저(P<0.01)；량개조별취간적간병리절편정상,간이식60 min,단순냉동보존조적간장출현교대면적적기중성립세포침윤,대면적배사,저온궤계관주보존조정상。단순냉동보존조화저온궤계관주보존조술후6 h내사망、존활≥5 d적견례수무현저차이(P>0.05),여단순냉동보존조비,술후3 d내출현사망례수현저강저(P<0.05),사망견균반유불동정도적황달。결론：저온궤계관주보존가현저강저CD 68화CRP,개선병리배사,제고성활솔。
Objective:To investigate and compare the CD 68 and CRP in the isolated liver between static cold storage and hypothermic machine perfusion before and after liver transplantation. Methods:The dogs were randomly divided into 2 groups:20 cases of static cold storage group and 20 cases of hy-pothermic machine perfusion group. The livers of static cold storage group were transplanted into hypothermic machine perfusion,and the livers of hypothermic machine perfusion were transplanted into static cold storage group. Results:CD 68,CRP and Fibulin-1 had no significant difference between the two groups as the live was isolated(P>0. 05). Before transplantation,CD 68 and Fibulin-1 in two groups had no significant difference(P>0. 05). Compared with cold storage group,CRP was decreased in the hypothermic machine perfusion group(P<0. 01). 60 min after transplantation,CD 68,CRP and Fibulin-1 were significantly reduced in hypothermic machine perfusion group compared with static cold storage group(P<0. 01). Before transplantation,liver pathological sections of two groups were normal. 60 min after transplantation,cold storage group liver appeared larger area of the neutrophil infiltration and necrosis. In hypothermic machine perfusion group,liver was relatively normal. Compared with cold storage group,cases alive within 3 days were higher in the hypothermic machine perfusion group( P<0. 05). Conclusion:Hypothermic machine perfusion can significantly reduce CD 68 and CRP,improve pathological neutrophil infiltration and necrosis,and improve the survival rate after transplantation.
