CAJ | 학술논문

背景：膝骨关节炎的主要改变是关节软骨面的退行性病变和继发性的骨质增生，病变的机制还不明确，但现已有实验证实膝骨关节炎的发病与炎症相关物质有密切的关系。 　　目的：分析炎症相关物质在膝骨关节炎发病机制中的作用。 　　方法：按美国风湿病学分会制定的诊断标准选择膝骨关节炎患者60例，来自因外伤截肢或半月板损伤性手术治疗的患者(排除膝关节内损伤)60例作为对照组，抽取两组患者膝关节液，采用酶联免疫吸附试验方法检测白细胞介素1β、白细胞介素6、白细胞介素8、白细胞介素10、肿瘤坏死因子α、碱性成纤维细胞生长因子、骨桥蛋白水平，采用一氧化氮检测试剂盒检测一氧化氮水平，按TBA荧光法测定过氧化脂质浓度。 　　结果与结论：膝骨关节炎患者表达高水平白细胞介素1β、白细胞介素6、白细胞介素8、白细胞介素10、肿瘤坏死因子α、碱性成纤维细胞生长因子、骨桥蛋白、一氧化氮和过氧化脂质水平均明显高于对照组；这些细胞因子及一氧化氮、过氧化脂质与膝骨关节炎的病变呈正相关。结果提示这些炎症相关物质确实参与了人膝骨关节炎的发病进程。
배경：슬골관절염적주요개변시관절연골면적퇴행성병변화계발성적골질증생，병변적궤제환불명학，단현이유실험증실슬골관절염적발병여염증상관물질유밀절적관계。 　　목적：분석염증상관물질재슬골관절염발병궤제중적작용。 　　방법：안미국풍습병학분회제정적진단표준선택슬골관절염환자60례，래자인외상절지혹반월판손상성수술치료적환자(배제슬관절내손상)60례작위대조조，추취량조환자슬관절액，채용매련면역흡부시험방법검측백세포개소1β、백세포개소6、백세포개소8、백세포개소10、종류배사인자α、감성성섬유세포생장인자、골교단백수평，채용일양화담검측시제합검측일양화담수평，안TBA형광법측정과양화지질농도。 　　결과여결론：슬골관절염환자표체고수평백세포개소1β、백세포개소6、백세포개소8、백세포개소10、종류배사인자α、감성성섬유세포생장인자、골교단백、일양화담화과양화지질수평균명현고우대조조；저사세포인자급일양화담、과양화지질여슬골관절염적병변정정상관。결과제시저사염증상관물질학실삼여료인슬골관절염적발병진정。
BACKGROUND:The main change of knee osteoarthritis is cartilaginous degeneration and secondary bone hyperplasia. The mechanism of these pathological changes is unclear, but some experiments have proved that the onset of knees osteoarthritis is closely related to inflammatory substances. OBJECTIVE:To discuss the role of inflammatory substances in the pathogenesis of knee osteoarthritis. METHODS:According to diagnostic criteria set by the American Colege of Rheumatology, we selected 60 knee osteoarthritis patients as experimental group, and another 60 patients undergoing the operative treatment of traumatic amputation or meniscus injury (except knee injury) as control group. After extracting synovial fluid samples from two groups, ELISA assay method was used to detect the levels of interleukin-1β, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor α, basic fibroblast growth factor, and osteopontin. The nitric oxide level in the synovial fluid was detected with nitric oxide detection kit. The content of lipid peroxidase was detected using TBA fluorescence method. RESULTS AND CONCLUSION:We found higher levels of interleukin-1β, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor α, basic fibroblast growth factor, osteopontin, nitric oxide, and lipid peroxidase in knee osteoarthritis patients, and these levels were positively correlated with knee osteoarthritis. These inflammatory substances are realy involved in the inflammation process of knees osteoarthritis.