CAJ | 학술논문

目的：探讨温针灸对兔膝关节软骨组织中基质金属蛋白酶-1(matrix metalloproteinase-1, MMP-1)和白细胞介素-1β(interleukin-1β, IL-1β)表达的影响。方法30只雄性成年日本大耳白兔采用随机数字表法分为正常组、模型组、治疗组各10只。除正常组外，其余2组兔通过膝关节强迫伸直位制动6周建立膝骨关节炎模型。模型制备成功后2周，治疗组取“内膝眼”“犊鼻”“足三里”“阳陵泉”行温针灸治疗4周；模型组和正常组不予干预。采用放免法检测膝关节软骨IL-1β、MMP-1水平。结果与正常组比较，模型组膝关节软骨 MMP-1水平[(0.37±0.02)ng/mg 比(0.16±0.02)ng/mg]、IL-1β水平[(0.34±0.02)pg/mg比(0.21±0.01)pg/mg]明显升高(P＜0.05)；治疗组膝关节软骨MMP-1[(0.28±0.03)ng/mg比(0.37±0.02)ng/mg，F＝258.251]水平、IL-1β水平[(0.31±0.04)pg/mg比(0.34±0.02)pg/mg，F＝127.112]较模型组显著降低(P＜0.01)。结论温针灸可降低兔制动引起的膝骨关节炎膝关节软骨中 MMP-1、IL-1β水平。
목적：탐토온침구대토슬관절연골조직중기질금속단백매-1(matrix metalloproteinase-1, MMP-1)화백세포개소-1β(interleukin-1β, IL-1β)표체적영향。방법30지웅성성년일본대이백토채용수궤수자표법분위정상조、모형조、치료조각10지。제정상조외，기여2조토통과슬관절강박신직위제동6주건립슬골관절염모형。모형제비성공후2주，치료조취“내슬안”“독비”“족삼리”“양릉천”행온침구치료4주；모형조화정상조불여간예。채용방면법검측슬관절연골IL-1β、MMP-1수평。결과여정상조비교，모형조슬관절연골 MMP-1수평[(0.37±0.02)ng/mg 비(0.16±0.02)ng/mg]、IL-1β수평[(0.34±0.02)pg/mg비(0.21±0.01)pg/mg]명현승고(P＜0.05)；치료조슬관절연골MMP-1[(0.28±0.03)ng/mg비(0.37±0.02)ng/mg，F＝258.251]수평、IL-1β수평[(0.31±0.04)pg/mg비(0.34±0.02)pg/mg，F＝127.112]교모형조현저강저(P＜0.01)。결론온침구가강저토제동인기적슬골관절염슬관절연골중 MMP-1、IL-1β수평。
Objective To investigate the effects of warming-needle acupuncture on the levels of matrix metalloproteinase-1 (MMP-1) and interleukin-1β (IL-1β) in the articular cartilage in knee osteoarthritis induced by immobilization in rabbits.MethodsA total of 30 adult male Japanese white rabbits were randomly assigned into three groups by random number table method: a normal group, a model group and a treatment group, with 10 rabbits in each group. Knee osteoarthritis was induced by 6 weeks of immobilization. The rabbits in the treatment group received warming-needle acupuncture after modeling at points of “Neixiyan” (EX-LE4), “Dubi” (ST35), “Zusanli” (ST36) and “Yanglingquan” (GB34) for 4 weeks. The level of MMP-1 and IL-1β in the articular cartilage of the knee were determined with radioimmunological assay.ResultsThere were significant difference in the levels of MMP-1(0.16 ± 0.02 ng/mg, 0.37 ± 0.02 ng/mg, and 0.28 ± 0.03 ng/mg;F=258.251) and IL-1β (0.21 ± 0.01 pg/mg, 0.34 ± 0.02pg/mg, and 0.31 ± 0.04 pg/mg;F=127.112) among the normal group, model group and treatment group. The levels of MMP-1 and IL-1β both in the model group and treatment group were significant higher than those in the normal group (allP<0.01), while the levels of MMP-1 and IL-1β in the treatment group were significant lower than those in the model group (allP<0.01).ConclusionWarming-needle acupuncture can effectively reduced the levels of MMP-1 and IL-1β in the articular cartilage in knee osteoarthritis induced by immobilization in rabbits.