CAJ | 학술논문

目的：建立夏枯草配方颗粒的质量标准。方法采用薄层色谱法鉴别夏枯草配方颗粒中的迷迭香酸，采用水溶性浸出物测定法中的热浸法测定夏枯草配方颗粒的浸出物，采用高效液相色谱法测定夏枯草配方颗粒中迷迭香酸的含量。结果薄层色谱斑点清晰，分离度好；浸出物不少于10.0%。含量测定中，迷迭香酸在0.0625～2.0080 mg/ml质量浓度范围内线性关系良好(r＝0.9998)，平均回收率为99.50%，RSD为1.21%，并初步确定夏枯草配方颗粒中迷迭香酸的含量应不得少于10.0 mg/袋。结论建立的定性定量方法操作方便、结果准确、专属性强，可用于夏枯草配方颗粒的质量控制。
목적：건립하고초배방과립적질량표준。방법채용박층색보법감별하고초배방과립중적미질향산，채용수용성침출물측정법중적열침법측정하고초배방과립적침출물，채용고효액상색보법측정하고초배방과립중미질향산적함량。결과박층색보반점청석，분리도호；침출물불소우10.0%。함량측정중，미질향산재0.0625～2.0080 mg/ml질량농도범위내선성관계량호(r＝0.9998)，평균회수솔위99.50%，RSD위1.21%，병초보학정하고초배방과립중미질향산적함량응불득소우10.0 mg/대。결론건립적정성정량방법조작방편、결과준학、전속성강，가용우하고초배방과립적질량공제。
Objective To establish the quality standard of common selfheal fruit-spike granules. Mehtods Rosmarinicacid in the granules were identified by TLC. The extractives were tested by the method for determination of water-soluble extractives. The contents of rosmarinicacid were determined by HPLC. Results TLC had obvious characteristics with the spots clear and well-separated. The extraction limit was no less than 10.0%. Rosmarinicacid showed a good linear relationship at 0.062 5~2.008 0 mg/ml (r=0.999 8,n=6) with average recovery of 99.50% (RSD=1.21%,n=9). The content limitation of rosmarinicacid was discussed as 10.0 mg per pack according to the experiment data.Conclusion The method is easy-operated and accurate which has a good specificity for the quality control of common selfheal fruit-spike granules.