CAJ | 학술논문

目的：本文通过比较黄芪及其不同提取物的红外光谱图，为黄芪的化学成分分析和提取分离工艺提供质量参考。方法利用乙醇回流法提取皂苷与黄酮，再用蒸馏水提取药渣中的多糖。对黄芪药材粉末、黄芪水提取物、黄芪醇提物及其药渣水提物的红外光谱及二阶导数谱进行分析。结果黄芪粉末、70%醇提物和80%醇提物皆于1735 cm-1附近(羰基伸缩振动吸收峰)有较弱、较宽的吸收峰，黄芪水提物中此波长附近的吸收峰不明显。表明黄芪水提物中主要为多糖类成分，含有少量不溶于水的黄酮类成分。醇提物则主要为皂苷类与黄酮类成分，且在一定范围内，黄酮类提取量随乙醇浓度增加而增加。结论红外光谱分析法可快速、简便地鉴别黄芪及其提取物的化学成分类别，为其进一步的质量研究提供依据。
목적：본문통과비교황기급기불동제취물적홍외광보도，위황기적화학성분분석화제취분리공예제공질량삼고。방법이용을순회류법제취조감여황동，재용증류수제취약사중적다당。대황기약재분말、황기수제취물、황기순제물급기약사수제물적홍외광보급이계도수보진행분석。결과황기분말、70%순제물화80%순제물개우1735 cm-1부근(탄기신축진동흡수봉)유교약、교관적흡수봉，황기수제물중차파장부근적흡수봉불명현。표명황기수제물중주요위다당류성분，함유소량불용우수적황동류성분。순제물칙주요위조감류여황동류성분，차재일정범위내，황동류제취량수을순농도증가이증가。결론홍외광보분석법가쾌속、간편지감별황기급기제취물적화학성분유별，위기진일보적질량연구제공의거。
Objective To provide effective reference for quality analysis of the chemical composition and extraction of astragalus separation process by comparing the extract of astragalus and it’s IR spectra. Methods The saponins and flavonoids in astragalus were firstly extracted by the method of circumfluence with ethanol as solvent and the residue of ethanol-extraction was then used to extract polysaccharides by distilled water. Fourier transform infrared spectroscopy (IR) combined with second derivative infrared spectroscopy was applied to quickly identify astragalus herbs powder, water extraction of astragalus, astragalus alcohol extraction and water extraction of the residue of ethanol-extraction. Results The powder and 70% ethanol extract, 80% ethanol extract were around at 1 735 cm-1 (carbonyl stretching vibration absorption peak) has a weak, broad absorption, while the absorption peak was less obvious in boiling water extraction. So the maln components of astragalus water extraction are polysaccharides and also contaln a small amount of water-insoluble flavonoids. Alcohol extraction malnly contalns saponins and flavonoids, and flavonoid extract volume increases with increasing alcohol concentration in a certaln range.Conclusion This method can be a quick and easy identification for astragalus and it’s extraction for its chemical composition class, and provide the basis for further research quality.