CAJ | 학술논문

目的：观察参麦注射液对局灶性脑缺血再灌注大鼠血管内皮功能的影响。方法将30只大鼠按随机数字表法分为假手术组、模型组、参麦治疗组各10只。除假手术组外，其余各组大鼠采用大脑中动脉线栓法制备局灶性脑缺血再灌注模型。各组于术后24 h开始给药，参麦治疗组大鼠腹腔注射参麦注射液2 ml/100 g，假手术组及模型组腹腔注射等体积生理盐水，1次/d，连续治疗7 d。于治疗前及治疗7 d后检测各组大鼠血浆内皮素-1(ET-1)、一氧化氮(NO)、一氧化氮合成酶活性(NOS)、超敏C-反应蛋白(hs-CRP)、同型半胱氨酸(Hcy)。结果参麦治疗组大鼠 ET-1[治疗前后分别为(126.10±6.07)ng/ml、(105.41±0.09)ng/ml]、hs-CRP[治疗前后分别为(15.93±2.79)mg/L、(9.33±1.32)mg/L]及Hcy[治疗前后分别为(11.01±0.98)μmol/L、(8.13±0.46)μmol/L]较治疗前降低， NOS[治疗前后分别为(168.87±9.25)U/ml、(242.25±10.36)U/ml]、NO[治疗前后分别为(84.03±6.02)nmol/ml、(102.42±5.05)nmol/ml]水平较治疗前升高，差异均有统计学意义(P＜0.05)。结论参麦注射液可提高 NOS 酶活性并促进 NO合成，降低血浆ET-1、hs-CRP及Hcy，减轻血管内皮炎性渗出，防止脑缺血再灌注损伤导致的血管内皮功能紊乱，对大鼠脑缺血再灌注后血管内皮具有保护作用。
목적：관찰삼맥주사액대국조성뇌결혈재관주대서혈관내피공능적영향。방법장30지대서안수궤수자표법분위가수술조、모형조、삼맥치료조각10지。제가수술조외，기여각조대서채용대뇌중동맥선전법제비국조성뇌결혈재관주모형。각조우술후24 h개시급약，삼맥치료조대서복강주사삼맥주사액2 ml/100 g，가수술조급모형조복강주사등체적생리염수，1차/d，련속치료7 d。우치료전급치료7 d후검측각조대서혈장내피소-1(ET-1)、일양화담(NO)、일양화담합성매활성(NOS)、초민C-반응단백(hs-CRP)、동형반광안산(Hcy)。결과삼맥치료조대서 ET-1[치료전후분별위(126.10±6.07)ng/ml、(105.41±0.09)ng/ml]、hs-CRP[치료전후분별위(15.93±2.79)mg/L、(9.33±1.32)mg/L]급Hcy[치료전후분별위(11.01±0.98)μmol/L、(8.13±0.46)μmol/L]교치료전강저， NOS[치료전후분별위(168.87±9.25)U/ml、(242.25±10.36)U/ml]、NO[치료전후분별위(84.03±6.02)nmol/ml、(102.42±5.05)nmol/ml]수평교치료전승고，차이균유통계학의의(P＜0.05)。결론삼맥주사액가제고 NOS 매활성병촉진 NO합성，강저혈장ET-1、hs-CRP급Hcy，감경혈관내피염성삼출，방지뇌결혈재관주손상도치적혈관내피공능문란，대대서뇌결혈재관주후혈관내피구유보호작용。
Objective To observe the effect ofShenmal injection on vascular endothelial function after cerebral ischemia reperfusion in rats.Methods 30 rats were randomly divided into a sham operation group, a model group and aShenmal group, with 10 in each. Except the sham operation group, animal models of cerebral ischemia reperfusion injury were established by the right internal carotid artery occlusion of middle cerebral artery. Then shenmal injection was given to theShenmal group, 2 ml/100 g Sodium Chloride was given to the other groups, once per day, for successive 7 days. Endothelin-1 (ET-1), nitric oxide (NO), nitric oxide synthase (NOS), serum high sensitivity C-reactive protein (hs-CRP), and homocysteine (Hcy) were tested. Results In Shenmal group, ET-1(126.10 ± 6.07 ng/mlvs.105.41 ± 0.09 ng/ml), hs-CRP (15.93 ± 2.79 mg/Lvs. 9.33 ± 1.32 mg/L) and Hcy (11.01 ± 0.98 μmol/Lvs. 8.13 ± 0.46 μmol/L) were decreased after the treatment, while NOS (168.87 ± 9.25 U/mlvs.242.25 ± 10.36 U/ml) and NO (84.03± 6.02vs.102.42± 5.05) were increased after the treatment with statistical difference(P<0.05).ConclusionShenmalinjection can improve NOS activity and promote the synthesis of NO, decrease the level of plasma ET-1, hs-CRP and Hcy, reduce endothelial inflammatory exudation, and prevent vascular endothelial dysfunction caused by cerebral ischemia reperfusion injury.