CAJ | 학술논문

目的：通过建立全身感染性疾病小鼠模型，探讨中性粒细胞明胶酶相关脂质运载蛋白（NGAL ）在鉴别诊断革兰阳性菌感染和革兰阴性菌感染方面的价值。方法选择216只IC R小鼠，每组8只，通过尾静脉分别注射金黄色葡萄球菌和大肠埃希菌，建立革兰阳性菌和革兰阴性菌所致的小鼠全身感染模型，分别在感染后1、3、6、12 h及1、2、3、5、7 d处死各组小鼠，检测其血清NGAL浓度。结果金黄色葡萄球菌感染组的小鼠NGAL浓度在感染后3 h明显升高，为（926．84±245．76）ng／ml ，大肠埃希菌感染组的小鼠NGAL浓度在感染后1 h明显升高，为（554．34±47．63）ng／ml；ROC曲线分析显示，金黄色葡萄球菌感染组和大肠埃希菌感染组 NGAL 的ROC曲线下面积（AUC）分别为0．908、0．979，两者差异无统计学意义。结论 NGAL在感染早期即明显升高，但其变化在金黄色葡萄球菌和大肠埃希菌引发的小鼠感染模型之间并无差异。
목적：통과건립전신감염성질병소서모형，탐토중성립세포명효매상관지질운재단백（NGAL ）재감별진단혁란양성균감염화혁란음성균감염방면적개치。방법선택216지IC R소서，매조8지，통과미정맥분별주사금황색포도구균화대장애희균，건립혁란양성균화혁란음성균소치적소서전신감염모형，분별재감염후1、3、6、12 h급1、2、3、5、7 d처사각조소서，검측기혈청NGAL농도。결과금황색포도구균감염조적소서NGAL농도재감염후3 h명현승고，위（926．84±245．76）ng／ml ，대장애희균감염조적소서NGAL농도재감염후1 h명현승고，위（554．34±47．63）ng／ml；ROC곡선분석현시，금황색포도구균감염조화대장애희균감염조 NGAL 적ROC곡선하면적（AUC）분별위0．908、0．979，량자차이무통계학의의。결론 NGAL재감염조기즉명현승고，단기변화재금황색포도구균화대장애희균인발적소서감염모형지간병무차이。
OBJECTIVE To explore the value of neutrophil gelatinase‐associated lipocalin(NGAL) in differential di‐agnosis of gram‐positive and gram‐negative bacteria infections by establishing systemic infectious mouse model . METHODS Totally 216 ICR mice were grouped ,with 8 mice in each group;the Staphylococcus aureus and Esche‐richia coli were respectively injected through tail vein so as to establish the gram‐positive bacteria‐and gram‐nega‐tive bacteria‐induced mice systemic infection model .The mice in each group were killed at 1 ,3 ,6 ,and 12 hours as well as at 1 ,2 ,3 ,5 ,and 7 days of being infected ,and the concentrations of serum NGAL were determined . RESULTS The concentration of NGAL of the mice infected with S .aureus was significantly elevated at 3 hours af‐ter being infected ,which was (926 .84 ± 245 .76)ng/ml;the concentration of NGAL of the mice infected with E .coli was remarkably elevated at 1 hour after being infected ,which was (554 .34 ± 47 .63)ng/ml .The ROC curve analysis indicated that the area under ROC curve of NGAL of the mice infected with S .aureus was 0 .908 , the mice infected with E .coli 0 .979 ,and there was no significant difference between the two .CONCLUSION The concentration of NGAL is significantly elevated at the early stage of infections ,however ,there is no significant difference in the change of concentration of NGAL between the mice infected with S .aureus and the mice infected with E .coli .