国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2015年
4期
289-293
,共5页
白芦藜醇%脓毒症%脑病%盲肠结扎穿孔%白细胞介素-1β
白蘆藜醇%膿毒癥%腦病%盲腸結扎穿孔%白細胞介素-1β
백호려순%농독증%뇌병%맹장결찰천공%백세포개소-1β
Resveratrol%Sepsis%Encephalopathy%Cecal ligation and puncture%Interleukin-1β
目的 探讨沉默信息调控因子1 (sirtuin 1,Sirt1)的天然激动剂白芦藜醇(resveratrol,Res)对脓毒症相关性脑病(sepsis-associated encephalopathy,SAE)炎性损伤的保护作用.方法 采用盲肠结扎穿孔(cecal ligation and puncture,CLP)方法制作脓毒症小鼠模型,18只C57B/L小鼠按随机数字表法分为3组,每组6只:假手术组(Sham组)、CLP脓毒症模型组(CLP组)以及CLP+Res注射组(Res组).术后24 h脑组织取材,末端脱氧核糖核苷酸转移酶介导的缺口末端标记法(terminaldeoxynucleotidyl transferase mediated nick end labelling,TUNEL)观察细胞凋亡情况;分离皮质和海马,采用实时定量聚合酶链式反应(real-time polymerase chain reaction,RT-PCR)分别检测Sirt1、含热蛋白结构域NOD样受体家族蛋白-3(NLR family pyrin domain containing-3 protein,NALP3)和白细胞介素(interleukin,IL)-1β的mRNA含量.结果 TUNEL染色后电镜下观察,海马Res组阳性细胞数量少于CLP组,凋亡细胞多集中于海马CA1区.Res组小鼠海马和皮质的Sirt1 mRNA的相对表达水平分别为4.62±0.64和4.60±0.61,显著高于Sham组和CLP组(P＜0.01).Res组海马和皮质的NALP3相对表达水平分别为0.86±0.26和0.94±0.35,显著低于CLP组(P＜0.05);与Sham组比较,差异无统计学意义(P＞0.05).Res组海马的IL-1β mRNA相对表达水平为0.57±0.17,显著低于Sham组和CLP组,差异有统计学意义(P＜0.01);Res组皮质的IL-1β mRNA相对表达水平为1.61±0.44,与Sham组和CLP组相似,差异无统计学意义(P＞0.05).结论 SAE时IL-1β在海马区引起的炎性损伤较皮质区明显,Sirt1激动剂白芦藜醇对SAE炎性损伤具有保护作用.
目的 探討沉默信息調控因子1 (sirtuin 1,Sirt1)的天然激動劑白蘆藜醇(resveratrol,Res)對膿毒癥相關性腦病(sepsis-associated encephalopathy,SAE)炎性損傷的保護作用.方法 採用盲腸結扎穿孔(cecal ligation and puncture,CLP)方法製作膿毒癥小鼠模型,18隻C57B/L小鼠按隨機數字錶法分為3組,每組6隻:假手術組(Sham組)、CLP膿毒癥模型組(CLP組)以及CLP+Res註射組(Res組).術後24 h腦組織取材,末耑脫氧覈糖覈苷痠轉移酶介導的缺口末耑標記法(terminaldeoxynucleotidyl transferase mediated nick end labelling,TUNEL)觀察細胞凋亡情況;分離皮質和海馬,採用實時定量聚閤酶鏈式反應(real-time polymerase chain reaction,RT-PCR)分彆檢測Sirt1、含熱蛋白結構域NOD樣受體傢族蛋白-3(NLR family pyrin domain containing-3 protein,NALP3)和白細胞介素(interleukin,IL)-1β的mRNA含量.結果 TUNEL染色後電鏡下觀察,海馬Res組暘性細胞數量少于CLP組,凋亡細胞多集中于海馬CA1區.Res組小鼠海馬和皮質的Sirt1 mRNA的相對錶達水平分彆為4.62±0.64和4.60±0.61,顯著高于Sham組和CLP組(P＜0.01).Res組海馬和皮質的NALP3相對錶達水平分彆為0.86±0.26和0.94±0.35,顯著低于CLP組(P＜0.05);與Sham組比較,差異無統計學意義(P＞0.05).Res組海馬的IL-1β mRNA相對錶達水平為0.57±0.17,顯著低于Sham組和CLP組,差異有統計學意義(P＜0.01);Res組皮質的IL-1β mRNA相對錶達水平為1.61±0.44,與Sham組和CLP組相似,差異無統計學意義(P＞0.05).結論 SAE時IL-1β在海馬區引起的炎性損傷較皮質區明顯,Sirt1激動劑白蘆藜醇對SAE炎性損傷具有保護作用.
목적 탐토침묵신식조공인자1 (sirtuin 1,Sirt1)적천연격동제백호려순(resveratrol,Res)대농독증상관성뇌병(sepsis-associated encephalopathy,SAE)염성손상적보호작용.방법 채용맹장결찰천공(cecal ligation and puncture,CLP)방법제작농독증소서모형,18지C57B/L소서안수궤수자표법분위3조,매조6지:가수술조(Sham조)、CLP농독증모형조(CLP조)이급CLP+Res주사조(Res조).술후24 h뇌조직취재,말단탈양핵당핵감산전이매개도적결구말단표기법(terminaldeoxynucleotidyl transferase mediated nick end labelling,TUNEL)관찰세포조망정황;분리피질화해마,채용실시정량취합매련식반응(real-time polymerase chain reaction,RT-PCR)분별검측Sirt1、함열단백결구역NOD양수체가족단백-3(NLR family pyrin domain containing-3 protein,NALP3)화백세포개소(interleukin,IL)-1β적mRNA함량.결과 TUNEL염색후전경하관찰,해마Res조양성세포수량소우CLP조,조망세포다집중우해마CA1구.Res조소서해마화피질적Sirt1 mRNA적상대표체수평분별위4.62±0.64화4.60±0.61,현저고우Sham조화CLP조(P＜0.01).Res조해마화피질적NALP3상대표체수평분별위0.86±0.26화0.94±0.35,현저저우CLP조(P＜0.05);여Sham조비교,차이무통계학의의(P＞0.05).Res조해마적IL-1β mRNA상대표체수평위0.57±0.17,현저저우Sham조화CLP조,차이유통계학의의(P＜0.01);Res조피질적IL-1β mRNA상대표체수평위1.61±0.44,여Sham조화CLP조상사,차이무통계학의의(P＞0.05).결론 SAE시IL-1β재해마구인기적염성손상교피질구명현,Sirt1격동제백호려순대SAE염성손상구유보호작용.
Objective To investigate the protective effect of sirtuin 1 (Sirt 1) agonist,resveratrol (Res),on the cerebral inflammatory damage in mouse with sepsis-associated encephalopathy(SAE).Methods Sepsis model was induced by cecal ligation and puncture (CLP).A total of 18 male C57BL/6 mice were divided into 3 groups randomly by the table of randomization:Sham group,CLP group and Res group (n=6).The brains were harvested 24 h after operation and apoptotic cell death was evaluated by terminal-deoxynucleotidyl transferase mediated nick end labelling (TUNEL) staining.The levels of Sirt1,NLR family pyrin domain containing-3 protein (NALP3) and interleukin (IL)-1β in hippocampus or cortex were measured using real-time polymerase chain reaction(RT-PCR).Results The number of TUNEL positive apoptotic cells in the hippocampal region was significantly less in Res group than that in CLP group.The apoptotic cells were more present in CA1 regions than in the cortex.The RT-PCR results showed that the expression levels of Sirtl mRNA in hippocampus and cortex in Res group were 4.62±0.64 and 4.60±0.61 respectively,significantly higher than that in Sham group and CLP group (P＜0.01).Levels of NALP3 mRNA in hippocampus and cortex in Res group were 0.86±0.26 and 0.94±0.35 respectively,which were significantly lower than that in the CLP group(P＜0.05),but similar to that in Sham group.IL-1β mRNA in hippocampus in Res group was 0.57±0.17,significantly lower than in Sham group and CLP group (P＜0.01).In cortex,IL-1β in Res group was 1.61±0.44,which was similar to that in Sham group and CLP group (P＞0.05).Conclusions Inflammation damage mediated by IL-1β occurs during SAE mainly in the hippocampal region compared with that in cortex and resveratrol plays a protective role against SAE.
