中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2015年
4期
240-243
,共4页
李存香%祁美英%熊浩明%杨晓艳%魏柏青%冯建萍%金星%辛有全%赵海红
李存香%祁美英%熊浩明%楊曉豔%魏柏青%馮建萍%金星%辛有全%趙海紅
리존향%기미영%웅호명%양효염%위백청%풍건평%금성%신유전%조해홍
耶尔森菌,鼠疫%毒力因子类%流行病学
耶爾森菌,鼠疫%毒力因子類%流行病學
야이삼균,서역%독력인자류%류행병학
Yersinia pestis%Virulence factors%Epidemiology
目的 研究我国各疫源地鼠疫耶尔森菌(简称鼠疫菌)毒力因子特征,为制订防治对策提供依据.方法 用常规方法对20世纪50年代以来,从我国11种不同的自然疫源地、不同的宿主和媒介分离的2 212株鼠疫菌进行4种毒力因子[荚膜抗原(F1)、毒力抗原(VW)、鼠疫菌素(PstⅠ)和色素沉着因子(Pgm)]检测,并采用x2检验,比较我国11种鼠疫自然疫源地分离鼠疫菌的毒力因子组成,以及自人间鼠疫患者及尸体分离鼠疫菌的毒力因子组成.结果 72.06％(1 594/2 212)的鼠疫菌4种毒力因子俱全.99.95％(2 211/2 212)的鼠疫菌F1阳性,99.64％(2 204/2 212)的鼠疫菌Pat Ⅰ阳性,73.73％(1 631/2212)的鼠疫菌VW阳性,72.06％(1 594/2 212)的鼠疫菌Pgm阳性,21.70％(480/2 212)的鼠疫菌Pgm阴性,Pgm混合型菌株占6.24％(138/2 212).11种鼠疫自然疫源地鼠疫菌株毒力因子F1和PstⅠ组成比较,差异无统计学意义(x2=0.61、3.64,P均＞0.05);VW、Pgm组成比较,差异有统计学意义(x2=227.99、390.96,P均＜0.05).除4种鼠疫自然疫源地无人间鼠疫病例外,其余7种鼠疫自然疫源地自人间鼠疫患者及尸体分离的220株鼠疫菌毒力因子VW、Pgm组成比较,差异有统计学意义(x2=39.72、51.05,P均＜0.05),而鼠疫菌F1、Pst Ⅰ阳性率均为100％.结论 我国不同疫源地分离的鼠疫菌绝大多数能产生毒力因子F1和Pst Ⅰ,性状稳定.不同疫源地鼠疫菌,不同疫源地人间鼠疫患者及尸体分离鼠疫菌毒力因子Pgm、VW在组成上有显著差别,二者与疫源地性质有一定的关系,具有鼠疫菌分型和流行病学意义.
目的 研究我國各疫源地鼠疫耶爾森菌(簡稱鼠疫菌)毒力因子特徵,為製訂防治對策提供依據.方法 用常規方法對20世紀50年代以來,從我國11種不同的自然疫源地、不同的宿主和媒介分離的2 212株鼠疫菌進行4種毒力因子[莢膜抗原(F1)、毒力抗原(VW)、鼠疫菌素(PstⅠ)和色素沉著因子(Pgm)]檢測,併採用x2檢驗,比較我國11種鼠疫自然疫源地分離鼠疫菌的毒力因子組成,以及自人間鼠疫患者及尸體分離鼠疫菌的毒力因子組成.結果 72.06％(1 594/2 212)的鼠疫菌4種毒力因子俱全.99.95％(2 211/2 212)的鼠疫菌F1暘性,99.64％(2 204/2 212)的鼠疫菌Pat Ⅰ暘性,73.73％(1 631/2212)的鼠疫菌VW暘性,72.06％(1 594/2 212)的鼠疫菌Pgm暘性,21.70％(480/2 212)的鼠疫菌Pgm陰性,Pgm混閤型菌株佔6.24％(138/2 212).11種鼠疫自然疫源地鼠疫菌株毒力因子F1和PstⅠ組成比較,差異無統計學意義(x2=0.61、3.64,P均＞0.05);VW、Pgm組成比較,差異有統計學意義(x2=227.99、390.96,P均＜0.05).除4種鼠疫自然疫源地無人間鼠疫病例外,其餘7種鼠疫自然疫源地自人間鼠疫患者及尸體分離的220株鼠疫菌毒力因子VW、Pgm組成比較,差異有統計學意義(x2=39.72、51.05,P均＜0.05),而鼠疫菌F1、Pst Ⅰ暘性率均為100％.結論 我國不同疫源地分離的鼠疫菌絕大多數能產生毒力因子F1和Pst Ⅰ,性狀穩定.不同疫源地鼠疫菌,不同疫源地人間鼠疫患者及尸體分離鼠疫菌毒力因子Pgm、VW在組成上有顯著差彆,二者與疫源地性質有一定的關繫,具有鼠疫菌分型和流行病學意義.
목적 연구아국각역원지서역야이삼균(간칭서역균)독력인자특정,위제정방치대책제공의거.방법 용상규방법대20세기50년대이래,종아국11충불동적자연역원지、불동적숙주화매개분리적2 212주서역균진행4충독력인자[협막항원(F1)、독력항원(VW)、서역균소(PstⅠ)화색소침착인자(Pgm)]검측,병채용x2검험,비교아국11충서역자연역원지분리서역균적독력인자조성,이급자인간서역환자급시체분리서역균적독력인자조성.결과 72.06％(1 594/2 212)적서역균4충독력인자구전.99.95％(2 211/2 212)적서역균F1양성,99.64％(2 204/2 212)적서역균Pat Ⅰ양성,73.73％(1 631/2212)적서역균VW양성,72.06％(1 594/2 212)적서역균Pgm양성,21.70％(480/2 212)적서역균Pgm음성,Pgm혼합형균주점6.24％(138/2 212).11충서역자연역원지서역균주독력인자F1화PstⅠ조성비교,차이무통계학의의(x2=0.61、3.64,P균＞0.05);VW、Pgm조성비교,차이유통계학의의(x2=227.99、390.96,P균＜0.05).제4충서역자연역원지무인간서역병예외,기여7충서역자연역원지자인간서역환자급시체분리적220주서역균독력인자VW、Pgm조성비교,차이유통계학의의(x2=39.72、51.05,P균＜0.05),이서역균F1、Pst Ⅰ양성솔균위100％.결론 아국불동역원지분리적서역균절대다수능산생독력인자F1화Pst Ⅰ,성상은정.불동역원지서역균,불동역원지인간서역환자급시체분리서역균독력인자Pgm、VW재조성상유현저차별,이자여역원지성질유일정적관계,구유서역균분형화류행병학의의.
Objective To study the characteristics of virulence factors of Yersiniapestis (Y.pestis) isolated from different natural plague foci in China and to provide a basis for developing prevention and control measures on plague.Methods Conventional technologies were applied to examine the characteristics of four virulence factors [Fra1 (F1),Pesticin1 (Pst Ⅰ),Virulence antigen (VW) and Pigmentation (Pgm)] of 2 212 strains of Y.pestis isolated from various host and media in different kinds natural foci since the 1950s,and chi-square test was used to contrast composition of virulence factors of Y.pestis from 11 different kinds of plague natural foci including the strains isolated from human plague patients and bodies.Results Among the 2 212 strains of Y.pestis,72.06％ (1 594/2 212) contained the four virulence factors,F1+ accounted for 99.95％ (2 211/2 212),Pst Ⅰ + accounted for 99.64％ (2 204/2 212),VW+accounted for 73.73％ (1 631/2 212),Pgm+ accounted for 72.06％ (1 594/2 212),Pgm-accounted for 21.70％ (480/2 212) and Pgm+/-accounted for 6.24％ (138/2 212),respectively.There were no significant differences (x2 =0.61,3.64,all P ＞ 0.05) on composition of virulence factors F1 and Pst Ⅰ of Y.pestis from 11 different kinds of plague natural foci,and significant differences (x2 =227.99,390.96,all P ＜ 0.05) on composition of virulence factors VW and Pgm of Y.pestis.In addition to 4 kinds of plague natural foci where no human plague cases were found,there were significant differences (x2 =39.72,51.05,all P ＜ 0.05) on composition of VW and Pgm of Y.pestis isolated from human plague patients and bodies in other 7 different kinds of plague natural foci; all the constituent ratios of F1+ and Pst Ⅰ + were 100％.Conclusions Most F1 and Pst Ⅰ of Y.pestis isolated from different kinds of natural plague foci in China are in a stable state.There are significant differences in the composition of Pgm and VW of Y.pestis isolated from plague patient in China,and the strains are related to plague foci and they have biovars,ecotype and epidemiological significance.
