国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2015年
3期
161-164
,共4页
马亚南%王宝红%苏庆红%许晓群%王郡甫
馬亞南%王寶紅%囌慶紅%許曉群%王郡甫
마아남%왕보홍%소경홍%허효군%왕군보
黑色素瘤%细胞增殖%细胞凋亡%转染%微小RNA-27a
黑色素瘤%細胞增殖%細胞凋亡%轉染%微小RNA-27a
흑색소류%세포증식%세포조망%전염%미소RNA-27a
Melanoma%Cell proliferation%Apoptosis%Transfection%microRNA-27a
目的:探讨微小RNA-27a( miR-27a)模拟物和抑制物转染黑色素瘤WM239细胞后对细胞增殖和凋亡的影响。方法将miR-27a模拟物、抑制物及其阴性对照转染WM239细胞,荧光显微镜观察转染效率,实时荧光定量PCR检测相应的微小RNA,四甲基偶氮唑盐( MTT)法检测细胞增殖,流式细胞仪检测细胞凋亡和细胞周期。结果细胞转染效率为80%~90%。转染miR-27a模拟物后,细胞内miR-27a表达量明显上升(2-△△CT值为26.98±0.01),与正常对照组相比差异有统计学意义( t=-1123.67,P=0.00);转染miR-27a抑制物后,细胞中miR-27a的表达量下降(2-△△CT值为0.96±0.02),与正常对照组相比差异无统计学意义(t=4.04,P=0.06)。转染miR-27a模拟物后,细胞增殖受到明显抑制,与正常对照组相比差异具有统计学意义[72 h吸光度(0.45±0.02)∶(0.72±0.01),F=129.56,P﹤0.05]。miR-27a模拟物组G0-G1期的细胞比例升高[(74.83±1.46)∶(63.73±1.25),F=30.33,P﹤0.05],S期和G2-M期细胞比例减少[(21.33±1.75)∶(27.50±1.25),F=14.98,P﹤0.05;(3.90±1.31)∶(8.80±2.10),F=3.66,P﹤0.05];模拟物组细胞凋亡率与正常对照组相比明显增加[(29.67±0.91)%∶(1.44±0.85)%, F=530.90,P﹤0.01];而抑制物组对细胞周期和凋亡无明显作用。结论 miR-27a抑制黑色素瘤细胞增殖,具有抑瘤作用,这与其促进细胞凋亡,阻滞细胞周期于G0-G1期相关。
目的:探討微小RNA-27a( miR-27a)模擬物和抑製物轉染黑色素瘤WM239細胞後對細胞增殖和凋亡的影響。方法將miR-27a模擬物、抑製物及其陰性對照轉染WM239細胞,熒光顯微鏡觀察轉染效率,實時熒光定量PCR檢測相應的微小RNA,四甲基偶氮唑鹽( MTT)法檢測細胞增殖,流式細胞儀檢測細胞凋亡和細胞週期。結果細胞轉染效率為80%~90%。轉染miR-27a模擬物後,細胞內miR-27a錶達量明顯上升(2-△△CT值為26.98±0.01),與正常對照組相比差異有統計學意義( t=-1123.67,P=0.00);轉染miR-27a抑製物後,細胞中miR-27a的錶達量下降(2-△△CT值為0.96±0.02),與正常對照組相比差異無統計學意義(t=4.04,P=0.06)。轉染miR-27a模擬物後,細胞增殖受到明顯抑製,與正常對照組相比差異具有統計學意義[72 h吸光度(0.45±0.02)∶(0.72±0.01),F=129.56,P﹤0.05]。miR-27a模擬物組G0-G1期的細胞比例升高[(74.83±1.46)∶(63.73±1.25),F=30.33,P﹤0.05],S期和G2-M期細胞比例減少[(21.33±1.75)∶(27.50±1.25),F=14.98,P﹤0.05;(3.90±1.31)∶(8.80±2.10),F=3.66,P﹤0.05];模擬物組細胞凋亡率與正常對照組相比明顯增加[(29.67±0.91)%∶(1.44±0.85)%, F=530.90,P﹤0.01];而抑製物組對細胞週期和凋亡無明顯作用。結論 miR-27a抑製黑色素瘤細胞增殖,具有抑瘤作用,這與其促進細胞凋亡,阻滯細胞週期于G0-G1期相關。
목적:탐토미소RNA-27a( miR-27a)모의물화억제물전염흑색소류WM239세포후대세포증식화조망적영향。방법장miR-27a모의물、억제물급기음성대조전염WM239세포,형광현미경관찰전염효솔,실시형광정량PCR검측상응적미소RNA,사갑기우담서염( MTT)법검측세포증식,류식세포의검측세포조망화세포주기。결과세포전염효솔위80%~90%。전염miR-27a모의물후,세포내miR-27a표체량명현상승(2-△△CT치위26.98±0.01),여정상대조조상비차이유통계학의의( t=-1123.67,P=0.00);전염miR-27a억제물후,세포중miR-27a적표체량하강(2-△△CT치위0.96±0.02),여정상대조조상비차이무통계학의의(t=4.04,P=0.06)。전염miR-27a모의물후,세포증식수도명현억제,여정상대조조상비차이구유통계학의의[72 h흡광도(0.45±0.02)∶(0.72±0.01),F=129.56,P﹤0.05]。miR-27a모의물조G0-G1기적세포비례승고[(74.83±1.46)∶(63.73±1.25),F=30.33,P﹤0.05],S기화G2-M기세포비례감소[(21.33±1.75)∶(27.50±1.25),F=14.98,P﹤0.05;(3.90±1.31)∶(8.80±2.10),F=3.66,P﹤0.05];모의물조세포조망솔여정상대조조상비명현증가[(29.67±0.91)%∶(1.44±0.85)%, F=530.90,P﹤0.01];이억제물조대세포주기화조망무명현작용。결론 miR-27a억제흑색소류세포증식,구유억류작용,저여기촉진세포조망,조체세포주기우G0-G1기상관。
Objective To investigate the effect of miR-27a mimic and inhibitor on proliferation and apoptosis in melanoma cell line WM239. Methods The miR-27a mimic,inhibitor and its negative control were transfected into WM239 cells. The transfection efficiency was evaluated by fluorescence microscope. The expres-sion of miR-27a was detected by real-time fluorescent quantitative PCR. The proliferation of cells was detected by MTT. The cell apoptosis and cell cycle were analyzed by flow cytometry. Results The transfection efficiency in WM239 cells was 80% to 90%. The expression of miR-27a was markedly up-regulated in miR-27a mimic group (2-△△CT value is 26. 98 ± 0. 01),with statistically significant difference(t= -1 123. 67,P=0. 00);and the miR-27a inhibitor group showed lower expression of miR-27a(2-△△CT value is 0. 96 ± 0. 02),there was no statisti-cally significant difference compared with normal control group(t=0. 04,P=0. 06). The proliferation of cells was obviously inhibited in miR-27a mimic group,and there was statistically significant difference compared with normal control group[absorbance of 72 h(0. 45 ± 0. 02)∶(0. 72 ± 0. 01),F=129. 56,P﹤0. 05]. The percent-age of WM239 cells in G0-G1 phase was increased[(74. 83 ± 1. 46)∶(63. 73 ± 1. 25),F=30. 33,P﹤0. 05], and the percentage of WM239 cells in S phase and G2-M phase were decreased[(21. 33 ± 1. 75)∶(27. 50 ± 1. 25),F=14. 98,P﹤0. 05;(3. 90 ± 1. 31)∶(8. 80 ± 2. 10),F=3. 66,P﹤0. 05]. The apoptosis rate of cells was significantly increased in miR-27a mimic group compared with normal group[(29. 67 ± 0. 91)%∶(1. 44 ± 0. 85)%,F=530. 90,P﹤0. 01],but the inhibitor group had no obvious effect on cell cycle and cell apoptosis. Conclusion MiR-27a can suppress melanoma cell proliferation and act as a tumor suppressor gene,which is rel-evant to induce cell apoptosis and block cell cycle in G0-G1 phase.