食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
7期
48-51
,共4页
大孔树脂%黄酮%土茯苓%落新妇苷
大孔樹脂%黃酮%土茯苓%落新婦苷
대공수지%황동%토복령%락신부감
macro-reticular resin%flavonoid%Glabrous Greenbrier Rhizome%Astilbin
通过测试树脂对土茯苓黄酮的吸附率、吸附速率、解吸率和产物得率及其黄酮含量，比较了5种不同类型吸附树脂对土茯苓中黄酮的吸附特性。结论5种树脂中D101树脂对土茯苓总黄酮的吸附效果最好，吸附量可达18.32 mg/g，X-5次之，吸附量达18.09 mg/g。动态柱吸附后，使用50%乙醇作为洗脱剂可以将被树脂吸附的黄酮全部洗下，用量为3.5倍柱体积。通过本工艺，2 kg土茯苓获得总黄酮6.27 g，得率为0.31%。经HPLC分析，总黄酮中落新妇苷含量为90.54%。
通過測試樹脂對土茯苓黃酮的吸附率、吸附速率、解吸率和產物得率及其黃酮含量，比較瞭5種不同類型吸附樹脂對土茯苓中黃酮的吸附特性。結論5種樹脂中D101樹脂對土茯苓總黃酮的吸附效果最好，吸附量可達18.32 mg/g，X-5次之，吸附量達18.09 mg/g。動態柱吸附後，使用50%乙醇作為洗脫劑可以將被樹脂吸附的黃酮全部洗下，用量為3.5倍柱體積。通過本工藝，2 kg土茯苓穫得總黃酮6.27 g，得率為0.31%。經HPLC分析，總黃酮中落新婦苷含量為90.54%。
통과측시수지대토복령황동적흡부솔、흡부속솔、해흡솔화산물득솔급기황동함량，비교료5충불동류형흡부수지대토복령중황동적흡부특성。결론5충수지중D101수지대토복령총황동적흡부효과최호，흡부량가체18.32 mg/g，X-5차지，흡부량체18.09 mg/g。동태주흡부후，사용50%을순작위세탈제가이장피수지흡부적황동전부세하，용량위3.5배주체적。통과본공예，2 kg토복령획득총황동6.27 g，득솔위0.31%。경HPLC분석，총황동중락신부감함량위90.54%。
Through the test of resin adsorption on Glabrous Greenbrier Rhizome flavonoids, adsorption rate, ratio of desorption, the yield rate and the content of flavonoid, Compared the adsorption characteristic of 5 different kinds of adsorption resins for flavonoids from Glabrous Greenbrier Rhizome. The best adsorption effect of five kinds of resin and D101 resin for total flavonoids in Glabrous Greenbrier Rhizome , adsorption capacity was 18.32 mg/g, and the X-5 followed, 18.09 mg/g. After the dynamic column adsorption, flavonoids using 50 % ethanol as eluant can be adsorbed by the resin eluted all yieid , the amount of 3.5 times the column volume. Through this process, 2 kg Glabzons Greenbiere Rhizowe 6.27 g flavonoids, yield 0.31 %. By the analysis of HPLC, total flavonoids astilbin content was 90.54%.
