响应面法优化紫薯花青素的超声波提取工艺
향응면법우화자서화청소적초성파제취공예
Optimization of Ultrasonic-assisted Extraction for Anthocyanins from Purple Sweet Potato by Response Surface Method Abstract:Ultrasound technology is a very useful way which is used to extract active ingredients from natural product. In this experiment, some factors affecting the ratio of extraction of proanthocyanidins such as extraction solvent ratio, extraction temperature, extraction time and ratio of material to liquid are studied respectively by ultrasound technology based on purple sweet potatos. The preparation process of anthocyanins from purple sweet potato was optimized according to Box-Behnken central composition design principle on foundation of single factor experiment, the method of response surface methodology (RSM) with 3 factors and 3 levels combined with Design-Expert data analysis software was adopted with the extraction yield of anthocyanins from purple sweet potato as the absorbance. The effect of several factors above on the efficiency of extraction of anthocyanins from purple sweet potato were studied in this paper. According to the above results, the optimum preparation conditions were obtained as follows:extraction temperature 49℃, extraction time 15 min,ratio of material to liquid 1∶41 (g/mL). Under these conditions, the absorbance of anthocyanins from purple sweet potato reached 1.45.
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