CAJ | 학술논문

通过比较不同甲酯化方法、气相色谱升温程序，确定了植物油中脂肪酸成分的气相色谱分析方法，并对5种食用植物油的主要脂肪酸含量进行了分析和比较。结果表明：三氟化硼-甲醇快速甲酯化法具有操作简单、时间短、甲酯化率高的优点。利用CP-Sill 88高极性气相色谱柱，优化的升温程序为：初始温度170℃，保持1 min，以10℃/min升温速率升至200℃，再以1℃/min升温速率升至220℃，保持3 min，20 min内即可有效分离6种脂肪酸。用建立的方法测定5种食用植物油6种脂肪酸的含量，标准曲线的相关性好，相关系数范围为0.9994~0.9999，检出限低。
통과비교불동갑지화방법、기상색보승온정서，학정료식물유중지방산성분적기상색보분석방법，병대5충식용식물유적주요지방산함량진행료분석화비교。결과표명：삼불화붕-갑순쾌속갑지화법구유조작간단、시간단、갑지화솔고적우점。이용CP-Sill 88고겁성기상색보주，우화적승온정서위：초시온도170℃，보지1 min，이10℃/min승온속솔승지200℃，재이1℃/min승온속솔승지220℃，보지3 min，20 min내즉가유효분리6충지방산。용건립적방법측정5충식용식물유6충지방산적함량，표준곡선적상관성호，상관계수범위위0.9994~0.9999，검출한저。
Different methods of methyl esterification and heating program of gas chromatography (GC) were compared, and optimal parameters for GC analysis of fatty acid in vegetable oil was determined. The content of essential fatty acids in 5 kinds of vegetable oil was also analyzed and compared. The results showed that boron trifluoride-methanol esterification was the optimal method for fatty acid analysis , which had advantages such as easy operation, accurate results with a high methyl esterification efficiency. A highly polar capillary column (100 m×0.25 mm i.d.×0.2 mm film thickness) of CP-Sil88 (Varian, USA) was used to separate the fatty acid methyl esters, the optimized heating program was as follows:1) 170℃for 1 min;2) increase to 200℃at a rate of 10℃/min;3) increase to 220℃at a rate of 10℃/min;4)220℃for 3 min, and 6 kinds of fatty acids can be effectively separated within 20 min. Determination of five kinds of edible vegetable oil 6 kinds of fatty acids by the established method showed a good linear ranges which correlation coefficient was from 0.999 4 to 0.999 9 and a low limit of detection.