中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2015年
4期
257-261,封3
,共6页
关节炎,实验性%磁共振成像%巨噬细胞%超微超顺磁氧化铁粒子%来氟米特
關節炎,實驗性%磁共振成像%巨噬細胞%超微超順磁氧化鐵粒子%來氟米特
관절염,실험성%자공진성상%거서세포%초미초순자양화철입자%래불미특
Arthritis,experimental%Magnetic resonance imaging%Macrophages%Ultrasmall super-paramagnetic iron oxide%Leflunomide
目的 应用胶原诱导性关节炎(CIA)大鼠关节滑膜巨噬细胞MRI技术,观察超微超顺磁性氧化铁粒子(USPIO)增强前后不同序列、不同时间点及药物干预后关节滑膜巨噬细胞MRI信号的改变.方法 将造模成功大鼠分为模型组、来氟米特组、模型对照组.模型组大鼠行USPIO增强前及增强后24、48、72 h MRI扫描;来氟米特组大鼠每日给药8 mg/kg,14 d后行USPIO增强前及增强后24 h MRI扫描.扫描序列为SE T1WI、SE T2WI、GRE T2* WI.各组大鼠扫描结束后留取膝关节滑膜行病理检查.多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验.结果 模型组T1WI增强前后各时间点未见明显滑膜信号改变,T2WI增强前及增强后24、48 h滑膜信噪比分别为24.13±1.96、17.09±1.23、19.14±0.91,T2*WI增强前及增强后24、48 h滑膜信噪比分别为22.28±0.92、11.40±0.53、17.18±0.63,T2WI及T2*WI增强后24、48 h滑膜信噪比较增强前差异均有统计学意义(P＜0.05);T2WI增强前后滑膜信噪比改变(△SNR)于增强后24、48、72 h分别为-29.1±2.4、-20.8±2.9、-6.2±2.9,T2 *WI增强前后滑膜△SNR于增强后24、48、72 h分别为-48.4±1.3、-22.9±0.8、-8.2±1.6,均于增强后24 h变化最明显(P＜0.05),病理示增强后24 h膝关节滑膜内铁粒子沉积较多;T2WI模型对照组及来氟米特组增强前后滑膜△SNR分别为-32.6±0.6、-29.0±0.8,T2 *WI模型对照组及来氟米特组增强前后滑膜△SNR分别为-44.2±1.2、-40.1±1.9,来氟米特组增强前后滑膜△SNR较模型对照组弱(P＜0.01),病理证实来氟米特组巨噬细胞及铁粒子沉积较模型对照组均减少.结论 USPIO增强磁共振对CIA大鼠关节滑膜巨噬细胞成像在SET2WI及GRE T2* WI序列表现为信号减低;USPIO增强后24 h MRI较48 h成像更佳;来氟米特干预后USPIO增强前后MRI滑膜信号改变减弱.关节滑膜巨噬细胞MRI有望成为临床RA诊断及药物疗效监测的新技术.
目的 應用膠原誘導性關節炎(CIA)大鼠關節滑膜巨噬細胞MRI技術,觀察超微超順磁性氧化鐵粒子(USPIO)增彊前後不同序列、不同時間點及藥物榦預後關節滑膜巨噬細胞MRI信號的改變.方法 將造模成功大鼠分為模型組、來氟米特組、模型對照組.模型組大鼠行USPIO增彊前及增彊後24、48、72 h MRI掃描;來氟米特組大鼠每日給藥8 mg/kg,14 d後行USPIO增彊前及增彊後24 h MRI掃描.掃描序列為SE T1WI、SE T2WI、GRE T2* WI.各組大鼠掃描結束後留取膝關節滑膜行病理檢查.多組間比較採用單因素方差分析,進一步兩兩比較採用LSD-t檢驗.結果 模型組T1WI增彊前後各時間點未見明顯滑膜信號改變,T2WI增彊前及增彊後24、48 h滑膜信譟比分彆為24.13±1.96、17.09±1.23、19.14±0.91,T2*WI增彊前及增彊後24、48 h滑膜信譟比分彆為22.28±0.92、11.40±0.53、17.18±0.63,T2WI及T2*WI增彊後24、48 h滑膜信譟比較增彊前差異均有統計學意義(P＜0.05);T2WI增彊前後滑膜信譟比改變(△SNR)于增彊後24、48、72 h分彆為-29.1±2.4、-20.8±2.9、-6.2±2.9,T2 *WI增彊前後滑膜△SNR于增彊後24、48、72 h分彆為-48.4±1.3、-22.9±0.8、-8.2±1.6,均于增彊後24 h變化最明顯(P＜0.05),病理示增彊後24 h膝關節滑膜內鐵粒子沉積較多;T2WI模型對照組及來氟米特組增彊前後滑膜△SNR分彆為-32.6±0.6、-29.0±0.8,T2 *WI模型對照組及來氟米特組增彊前後滑膜△SNR分彆為-44.2±1.2、-40.1±1.9,來氟米特組增彊前後滑膜△SNR較模型對照組弱(P＜0.01),病理證實來氟米特組巨噬細胞及鐵粒子沉積較模型對照組均減少.結論 USPIO增彊磁共振對CIA大鼠關節滑膜巨噬細胞成像在SET2WI及GRE T2* WI序列錶現為信號減低;USPIO增彊後24 h MRI較48 h成像更佳;來氟米特榦預後USPIO增彊前後MRI滑膜信號改變減弱.關節滑膜巨噬細胞MRI有望成為臨床RA診斷及藥物療效鑑測的新技術.
목적 응용효원유도성관절염(CIA)대서관절활막거서세포MRI기술,관찰초미초순자성양화철입자(USPIO)증강전후불동서렬、불동시간점급약물간예후관절활막거서세포MRI신호적개변.방법 장조모성공대서분위모형조、래불미특조、모형대조조.모형조대서행USPIO증강전급증강후24、48、72 h MRI소묘;래불미특조대서매일급약8 mg/kg,14 d후행USPIO증강전급증강후24 h MRI소묘.소묘서렬위SE T1WI、SE T2WI、GRE T2* WI.각조대서소묘결속후류취슬관절활막행병리검사.다조간비교채용단인소방차분석,진일보량량비교채용LSD-t검험.결과 모형조T1WI증강전후각시간점미견명현활막신호개변,T2WI증강전급증강후24、48 h활막신조비분별위24.13±1.96、17.09±1.23、19.14±0.91,T2*WI증강전급증강후24、48 h활막신조비분별위22.28±0.92、11.40±0.53、17.18±0.63,T2WI급T2*WI증강후24、48 h활막신조비교증강전차이균유통계학의의(P＜0.05);T2WI증강전후활막신조비개변(△SNR)우증강후24、48、72 h분별위-29.1±2.4、-20.8±2.9、-6.2±2.9,T2 *WI증강전후활막△SNR우증강후24、48、72 h분별위-48.4±1.3、-22.9±0.8、-8.2±1.6,균우증강후24 h변화최명현(P＜0.05),병리시증강후24 h슬관절활막내철입자침적교다;T2WI모형대조조급래불미특조증강전후활막△SNR분별위-32.6±0.6、-29.0±0.8,T2 *WI모형대조조급래불미특조증강전후활막△SNR분별위-44.2±1.2、-40.1±1.9,래불미특조증강전후활막△SNR교모형대조조약(P＜0.01),병리증실래불미특조거서세포급철입자침적교모형대조조균감소.결론 USPIO증강자공진대CIA대서관절활막거서세포성상재SET2WI급GRE T2* WI서렬표현위신호감저;USPIO증강후24 h MRI교48 h성상경가;래불미특간예후USPIO증강전후MRI활막신호개변감약.관절활막거서세포MRI유망성위림상RA진단급약물료효감측적신기술.
Objective To explore the optimal time and sequence for getting the best magnetic resonance (MR) imaging when MR image of synovium macrophages was used for the diagnosis of collageninduced arthritis (CIA) in a rat model,and whether it can be used to monitor the efficacy of drug treatment.Methods CIA was induced by subcutaneous injection of chicken type Ⅱ collagen and complete Freund's adjuvant (CFA).Arthritis rats were randomly divided into the model group,the leflunomide group and the control group.Knees of the model group rats were imaged before and 24 h,48 h,72 h after USPIO intravenous administration (300 μmol Fe/kg) on day 28,29,30,31,respectively.From day 28,the leflunomide group was given a gauge of drug at a dose of 8 mg/kg.Then they were imaged before and 24 hours after USPIO administration on day 42,43 respectively.MR sequences included SE T1WI,SE T2WI,GRE T2 * WI.After the completion of MR imaging,rats were sacrificed to obtain histopathologic samples of synovial membrane.LSD-t test and one-way analysis of variance (ANOVA) were used for statistical analysis.Results No distinct signal enhancements were observed on the 24 h,48 h,72 h post-contrast enhancement on T1WI.On T2WI,signal intensity ratio of synovium (SNR) pre-contrast and 24 h,48 h post-contrast were 24.13±1.96,17.09± 1.23,19.14±0.91,respectively.On T2 * WI,SNR pre-contrast and 24 h,48 h post-contrast were 22.28±0.92,11.40±0.53,17.18±0.63,respectively.Distinct signal changes were observed on 24 h,48 h post-contrast on T2WI and T2 * WI (P＜0.05).The changes between SNR at 24 h,48 h,72 h post-contrast and pre-contrast were-29.09±2.42,-20.83±2.90,-6.2±2.9 respectively on T2WI,which were-48.4±1.3,-22.9±0.8,-8.2±1.6 respectively on T2 * WI.Changes were more obvious at 24 h post-contrast than 48 h post-contrast on both T2WI and T2 * WI (P＜0.05).The quantitative analyses were coinci-dent with the visual differences in signal changes between pre-contrast and post-contrast images.Difference between △SNR of leflunomide group and the control group on T1WI was not significant,while that on T2WI and T2 * WI were significantly different (P＜ 0.01).Histological examination confirmed the uptake of iron in the macrophages of arthritic knees.Signal intensity changed more on GRE T2 * WI than SE T2WI in all arthritis rats.Conclusion GRE T2 * WI is more sensitive for the diagnose of rat CIA,and 24 h post-contrast is better than 48 h and 72h post-contrast to get better images.We successfully observed the effects of leflunomide through signal changes of synovium,and the technique maybe contribute to diagnosis and therapeutic monito-ring of rheumatoid arthritis.
