CAJ | 학술논문

鸭瘟是危害养鸭业的一种重要传染病。本文概述了鸭瘟病毒（DPV）实验室检测方法的研究进展。在细胞生物学方面，主要依靠病毒分离培养、ELISA法、血清中和试验等病毒特异性抗原及其抗体的检测等。在分子生物学方面，主要依靠国内外相继建立起的PCR技术、实时荧光定量RT-PCR技术、LAMP技术和核酸杂交技术等。介绍和比较了各种方法的优缺点和实用性，为临床科技工作者建立快速、简便、准确、实用的检测方法提供参考。
압온시위해양압업적일충중요전염병。본문개술료압온병독（DPV）실험실검측방법적연구진전。재세포생물학방면，주요의고병독분리배양、ELISA법、혈청중화시험등병독특이성항원급기항체적검측등。재분자생물학방면，주요의고국내외상계건립기적PCR기술、실시형광정량RT-PCR기술、LAMP기술화핵산잡교기술등。개소화비교료각충방법적우결점화실용성，위림상과기공작자건립쾌속、간편、준학、실용적검측방법제공삼고。
Duck plague (DP)is one of the main infectious diseases which is a serious harm to duck industry. The development of laboratory methods of the Duck plague virus (DPV)was summarized in this paper. In the cell biology aspect, the diagnosis of DPV was mainly depended on virus isolation and culture, specific antigen and antibody examination such as ELISA and serum neutralization test. In the molecular biology aspect, mainly depended on RT -PCR and real -time RT -PCR diagnosis, LAMP and nucleic acid hybridization. The advantages and disadvantages, and the practicality of the several of methods were introduced and compared. It was to be provided reference for clinical veterinary technology workers to establish of rapid, simple, accurate and practical diagnosis method.