CAJ | 학술논문

评价乳源肽α2-f（56-57）：YS的血管紧张素转化酶（ACE）活性抑制作用及降压效果，并将其应用于发酵酸奶，制备具有降压作用的发酵酸奶。采用分光光度法检测α2-f（56-57）：YS对ACE活性的抑制作用，用不同剂量的α2-f（56-57）：YS及添加α2-f（56-57）：YS的发酵酸奶灌胃原发性高血压大鼠（SHR），评价其降血压效果。结果表明，片段α2-f（56-57）：YS具有很好的ACE活性抑制作用，其IC50值为11.89μg/mL；体内检测表明，用低、中、高（1、3、9 mg/kg mb）剂量灌胃原发性高血压大鼠（SHR），均显示有降血压作用，在灌胃后4 h，其血压分别降低了18.25、31.38和36.88 mm Hg，8 h后恢复到初始血压值。添加α2-f（56-57）：YS的发酵酸奶，以10 mL/kg mb的剂量灌胃SHR，在灌胃后4 h，对SHR的收缩压（SBP）降低了32.5mm Hg，8 h后恢复到初始值。因此，α2-f（56-57）：YS具有降血压作用，应用于发酵酸奶中，可制备具有降压功能的酸奶。
평개유원태α2-f（56-57）：YS적혈관긴장소전화매（ACE）활성억제작용급강압효과，병장기응용우발효산내，제비구유강압작용적발효산내。채용분광광도법검측α2-f（56-57）：YS대ACE활성적억제작용，용불동제량적α2-f（56-57）：YS급첨가α2-f（56-57）：YS적발효산내관위원발성고혈압대서（SHR），평개기강혈압효과。결과표명，편단α2-f（56-57）：YS구유흔호적ACE활성억제작용，기IC50치위11.89μg/mL；체내검측표명，용저、중、고（1、3、9 mg/kg mb）제량관위원발성고혈압대서（SHR），균현시유강혈압작용，재관위후4 h，기혈압분별강저료18.25、31.38화36.88 mm Hg，8 h후회복도초시혈압치。첨가α2-f（56-57）：YS적발효산내，이10 mL/kg mb적제량관위SHR，재관위후4 h，대SHR적수축압（SBP）강저료32.5mm Hg，8 h후회복도초시치。인차，α2-f（56-57）：YS구유강혈압작용，응용우발효산내중，가제비구유강압공능적산내。
Objective of this research was to evaluate inhibition effect of α2-f(56-57):YS, which from milkα2-casein, on ACE (angiotensin converting enzyme)activity, and its antihypertensive efficacy. And it was applied to produce yoghourt, which had antihypertensive efficacy. The inhibition effect of α2-f (56-57):YS on ACE activity was evaluated using spectrophotometric method. The antihypertensive efficacy of α2-f(56-57):YS and the yoghourt which contain α2-f(56-57):YS was evaluated by gavage SHR(pontaneously hypertensive rat) in different dose. Results showed thatα2-f(56-57):YS had good inhibition effect on ACE activity in vitro, and its median inhibitory concentration (IC50) was 11.89μg/mL. SHR was given α2-f (56-57):YS in different dose (1, 3, 9 mg/kg mb) by gavage, after given 4 hour, the blood pressure of SHR was reduced 18.25, 31.38 and 36.88 mm Hg, respectively, and it was return to the initial blood pressure values after given 8 hour. SHR was given the yoghourt of containing α2-f (56-57):YS in 10 mL/kg mb by gavage, after given 4 h, its SBP was reduced 32.5 mm Hg, and after given 8 h, the SBP return to the initial value. Therefore,α2-f (56-57):YS had antihypertensive efficacy, and applied it in yoghurt, could give the yoghourt antihypertensive efficacy.