CAJ | 학술논문

采用酸法、碱法、酶法、水法从鱼皮中提取胶原蛋白，对其性质进行分析比较。结果表明，提取率，酸法＞酶法＞碱法＞水法；热变性温度，水法＞酶法＞碱法＞酸法；水法和碱法提取的胶原蛋白SDS-PAGE电泳图谱条带中出现β二聚体和γ三聚体，酶法和酸法没有出现，但是酶法和酸法的α链位置明显色带浓于水法和碱法；紫外吸收光谱中，酸法和碱法的吸收峰强度大于水法和酶法，碱法和酶法左侧出现了杂峰；红外光谱显示，碱法提取的鱼皮胶原蛋白不具备酰胺带特征吸收峰，其二级结构已破坏，酸法、酶法和水法提取的胶原蛋白均存在酰胺带特征吸收峰，而且出现位置相似，符合胶原蛋白特征吸收峰。
채용산법、감법、매법、수법종어피중제취효원단백，대기성질진행분석비교。결과표명，제취솔，산법＞매법＞감법＞수법；열변성온도，수법＞매법＞감법＞산법；수법화감법제취적효원단백SDS-PAGE전영도보조대중출현β이취체화γ삼취체，매법화산법몰유출현，단시매법화산법적α련위치명현색대농우수법화감법；자외흡수광보중，산법화감법적흡수봉강도대우수법화매법，감법화매법좌측출현료잡봉；홍외광보현시，감법제취적어피효원단백불구비선알대특정흡수봉，기이급결구이파배，산법、매법화수법제취적효원단백균존재선알대특정흡수봉，이차출현위치상사，부합효원단백특정흡수봉。
Extract collagen of grass carp's skin with acid, alkali ,enzyme and water method, the result of collagen's extraction yield in different method was as follow:acid>alkali>enzyme>water. The result of collagen's in different method was as follow: alkali >acid >water >enzyme. The result of collagen's SDS -PAGE electrophoresis was: the ribbons from water and alkali appeared in β-dipolymer and γ-tripolymer ribbons position, the ribbons from enzyme and acid no appearance, butα-chain's ribbons from enzyme and acid marks significantly thicker than ribbons from water and alkali. The result of collagen's UV scanning spectrum was:UV absorption intensity from acid and alkali were greater than that from water and enzyme , the maximum UV absorption left side from alkali and enzyme appeared some miscellaneous peaks. The result of collagen's FI-IR spectrum was: according with the characteristic absorption peak. The collagen extracted in four methods all have characteristic absorption peaks which are amideⅠ, amideⅡ, amideⅢ, and their position are similar to each other, It was in accordance withⅠcollagen's characteristic absorption peak.