CAJ | 학술논문

采用超声萃取-紫外分光光度法测定五种不同的茶叶中咖啡因的含量，结果表明：最佳超声提取条件：超声功率150 W，温度60℃，时间30 min；5种不同茶叶咖啡因含量顺序为：粗老绿茶﹥普洱﹥金骏眉﹥信阳毛尖﹥铁观音。紫外分光光度计法显示：水溶剂咖啡因的最大吸收波长为273 nm，线性回归方程为：A=0.04855C-0.02854，r为0.9992，浓度在0.00～25.00μg/mL，线性关系良好，平均回收率为96.42%，RSD﹤2.0%。该方法简便快速，准确，可用于茶叶中咖啡因含量测定。
채용초성췌취-자외분광광도법측정오충불동적다협중가배인적함량，결과표명：최가초성제취조건：초성공솔150 W，온도60℃，시간30 min；5충불동다협가배인함량순서위：조로록다﹥보이﹥금준미﹥신양모첨﹥철관음。자외분광광도계법현시：수용제가배인적최대흡수파장위273 nm，선성회귀방정위：A=0.04855C-0.02854，r위0.9992，농도재0.00～25.00μg/mL，선성관계량호，평균회수솔위96.42%，RSD﹤2.0%。해방법간편쾌속，준학，가용우다협중가배인함량측정。
By ultrasonic extraction and UV spectrophotometry detection the caffeine contents in five different types of tea were detected. The results showed the optimum ultrasonic extraction conditions: power 150 W, temperature 60℃, time of 30 min;the contentions of caffeine in five different tea are coarse old green tea﹥Pu 'er tea﹥Jinjunmei﹥Xingyang maojian﹥Tie Guanyin. The max absorbance of caffeine was detected by ultraviolet spectrophotometry at wavelength 273 nm.The relationship of the absorbance (A) and the concentration (C) was linear from 0.00 to 25.00μg/mL (r=0.999 2), The linear equation was A=0.048 55C-0.028 54.The average recovery was 95.46%,the relative standard deviations were less than 2.0%.This method can he used for the determination of caffeine in tea because of its its simple ,rapid and accurate characteristics.