CAJ | 학술논문

试验分别采用组织块贴壁法和消化分离法对奶山羊胎儿成纤维细胞进行体外培养，通过原代培养、细胞传代、冷冻保存等成功建立了奶山羊胎儿成纤维细胞系，并对该细胞系进行了形态学观察、生长动力学分析、细胞活力测定、核型分析和微生物检测等多种生物学特性分析.结果表明：成纤维细胞原代培养采用贴块培养时所需时间较长，消化培养有较多死细胞；传代细胞生长情况良好，群体倍增时间(PDT)约为40.4 h；生长总体趋势呈“S”型；冻存后活力为92.3%，生长状况与冻存前一致；细胞染色体中二倍体(2 n=60)占主体；细菌、真菌和支原体检测结果均为阴性，细胞系的各项指标均达到 ATCC 细胞系鉴定标准.
시험분별채용조직괴첩벽법화소화분리법대내산양태인성섬유세포진행체외배양，통과원대배양、세포전대、냉동보존등성공건립료내산양태인성섬유세포계，병대해세포계진행료형태학관찰、생장동역학분석、세포활력측정、핵형분석화미생물검측등다충생물학특성분석.결과표명：성섬유세포원대배양채용첩괴배양시소수시간교장，소화배양유교다사세포；전대세포생장정황량호，군체배증시간(PDT)약위40.4 h；생장총체추세정“S”형；동존후활력위92.3%，생장상황여동존전일치；세포염색체중이배체(2 n=60)점주체；세균、진균화지원체검측결과균위음성，세포계적각항지표균체도 ATCC 세포계감정표준.
The primary cells of dairy goat fetus were obtained by methods of tissue culture and collage-nase dissociation,then using the method of primary culture,serial subcultivation and cryopreservation we established a dairy goat fetus fibroblast cell line successfully.The morphological observations,dynamic growth analysis,determination of viability,karyotype analysis,microorganism test were all done to study the biological characteristics of the cell line.The results showed that primary culture with explants needed more time,while culture with trypsinization had more dead cells.The passage cell growth in good condi-tion,the population doubling time (PDT)of cells was approximately reached to 40.4 h.Cell growth tend-ency presented as ‘S’curve.The cell viability was 92.3% after anabiosisand and maintained the same growth condition.Analysis of chromosomal karyotype indicated that diploid (2 n=60)hold a predominance place in the cell line.The detection results of the bacteria,fungus and mycoplasmata were all negative.Ev-ery index in the cell line met all the standard quality controls of ATCC.It is valuable to provide the desira-ble subject for bioresearch and ideal the experimental material to construct animal mammary gland bioreac-tor.