CAJ | 학술논문

为了比较IGF-Ⅰ和IGF-ⅠR 基因在绵羊肌肉中的表达差异,根据 GenBank 公布的绵羊IGF-Ⅰ和IGF-ⅠR基因 mRNA 序列设计特异性引物,采用实时定量 PCR 方法检测其在甘肃‘高山细毛羊’及其与南非肉用‘美利奴’杂交 F1代(南甘 F1代)羔羊肌肉组织中的表达量.利用生物信息学软件分析了绵羊IGF-Ⅰ和IGF-ⅠR 基因结构,并构建系统进化树,探讨了其生物学功能.结果表明：南甘 F1代羔羊肌肉组织中IGF-Ⅰ基因的表达量显著高于‘甘肃高山细毛羊’(P <0.05),而IGF-ⅠR 基因的表达量在南甘 F1代羔羊和‘甘肃高山细毛羊’肌肉组织中没有显著变化(P >0.05).绵羊IGF-Ⅰ和IGF-ⅠR 基因分别含有465 bp 和4518 bp 的完整开放阅读框,编码154个和1505个氨基酸；其蛋白质分子量分别为17011.71 U 和169267.26 U,等电点分别为9.36和6.70；IGF-Ⅰ基因信号肽切割点分别位于49~50位氨基酸之间,而IGF-ⅠR 基因不含信号肽；IGF-Ⅰ基因编码产物的二级结构主要以无规则卷曲和α-螺旋为主,位于细胞核的可能性最高(62.5%)；IGF-ⅠR 基因编码产物的二级结构主要以无规则卷曲为主,位于细胞质的可能性最高(34.8%)；IGF-Ⅰ和IGF-ⅠR 基因分别作为激素和信号传感器参与机体生物学过程.
위료비교IGF-Ⅰ화IGF-ⅠR 기인재면양기육중적표체차이,근거 GenBank 공포적면양IGF-Ⅰ화IGF-ⅠR기인 mRNA 서렬설계특이성인물,채용실시정량 PCR 방법검측기재감숙‘고산세모양’급기여남비육용‘미리노’잡교 F1대(남감 F1대)고양기육조직중적표체량.이용생물신식학연건분석료면양IGF-Ⅰ화IGF-ⅠR 기인결구,병구건계통진화수,탐토료기생물학공능.결과표명：남감 F1대고양기육조직중IGF-Ⅰ기인적표체량현저고우‘감숙고산세모양’(P <0.05),이IGF-ⅠR 기인적표체량재남감 F1대고양화‘감숙고산세모양’기육조직중몰유현저변화(P >0.05).면양IGF-Ⅰ화IGF-ⅠR 기인분별함유465 bp 화4518 bp 적완정개방열독광,편마154개화1505개안기산；기단백질분자량분별위17011.71 U 화169267.26 U,등전점분별위9.36화6.70；IGF-Ⅰ기인신호태절할점분별위우49~50위안기산지간,이IGF-ⅠR 기인불함신호태；IGF-Ⅰ기인편마산물적이급결구주요이무규칙권곡화α-라선위주,위우세포핵적가능성최고(62.5%)；IGF-ⅠR 기인편마산물적이급결구주요이무규칙권곡위주,위우세포질적가능성최고(34.8%)；IGF-Ⅰ화IGF-ⅠR 기인분별작위격소화신호전감기삼여궤체생물학과정.
In order to compare the different expression ofIGF-Ⅰ andIGF-ⅠR genes in muscle tissue of two sheep breeds,‘Gansu Alpine Merino’and its F1 hybrids with ‘South African Meat Merino’,different primers were designed according to the mRNA sequences of these two genes in GeneBank,and their expres-sion quantity was determined by real-time quantitative PCR.The structure and function of the IGF-Ⅰ and IGF-ⅠR gene-encoded protein were predicted by the bioinformatics software and the phylogenetic trees of these two genes were constructed.The results showed that:IGF-Ⅰ gene expression quantity in muscle tis-sue of the F1 hybrids was significantly higher than that of ‘Gansu Alpine Merino’(P <0.05),while IGF-ⅠR gene expression quantity in muscle tissue showed no difference between the F1 hybrids and ‘Gansu Al-pine Merino’(P >0.05).IGF-Ⅰ andIGF-ⅠR gene both including complete open reading frame of 465 bp and 4 5 18 bp,which encoded 154 and 1 505 amino acids respectively,and their protein molecular weight was 17 01 1.71 U and 1 69 267.26 U,isoelectric point was 9.36 and 6.70.The protein of IGF-Ⅰ had one signal peptide between site 49 and 50,while the protein of IGF-ⅠR had no signal peptide.The secondary struc-ture ofIGF-Ⅰ gene coding product was mainly constituted ofα-helix and random coil,and it most probably (possibility of 62.5%)located in nucleus.However,the secondary structure ofIGF-ⅠR gene coding prod-uct was mainly constituted of random coil,and it most probably (possibility of 34.8%)located in cyto-plasm.IGF-Ⅰ andIGF-ⅠR gene may act as hormone and signal sensor in biological processes,respectively.