CAJ | 학술논문

目的建立黑果枸杞的质量控制标准。方法采用HPLC‐DAD（高效液相色谱法‐二极管阵列检测器），以C18 ODS 80 Ts QA（150mm×4．9mm，7μm）为色谱柱，以100mL·L －1甲酸溶液（1mL·L －1三氟乙酸）‐乙腈（150mL·L －1甲醇）为流动相，梯度洗脱，检测波长525 nm ，流速1．0 mL · min－1，柱温：30℃，对黑果枸杞中花青素类天然色素成分进行了半定量分析及指纹图谱研究。采用HPLC‐ELSD（高效液相色谱法‐蒸发光检测器），以 Hypersil NH2（150 mm ×4．2 mm ，5μm）柱为色谱柱，以四氢呋喃‐甲醇‐30 mL · L －1三氟乙酸（61∶30∶9）为流动相，等度洗脱，流速为1．0 mL · min－1，载气流速为2．5 mL · min－1，漂移管温度是90℃；柱温22℃，对甜菜碱成分进行定量分析。结果花青素类成分在2．725～27．25μg范围内具有良好的线性关系， r＝0．9999）；平均回收率为101．6％，RSD为1．0％；花青素类成分指纹图谱有13个共有峰，方法学考察结果良好，各产地黒果枸杞指纹图相似度都大于0．99。甜菜碱在2．07～20．68μg范围内具有良好的线性关系（ r＝0．9997），平均回收率为101．1％，RSD为1．4％。结论所建立的方法可靠、稳定，可用于黒果枸杞质量的全面评价。
목적건립흑과구기적질량공제표준。방법채용HPLC‐DAD（고효액상색보법‐이겁관진렬검측기），이C18 ODS 80 Ts QA（150mm×4．9mm，7μm）위색보주，이100mL·L －1갑산용액（1mL·L －1삼불을산）‐을정（150mL·L －1갑순）위류동상，제도세탈，검측파장525 nm ，류속1．0 mL · min－1，주온：30℃，대흑과구기중화청소류천연색소성분진행료반정량분석급지문도보연구。채용HPLC‐ELSD（고효액상색보법‐증발광검측기），이 Hypersil NH2（150 mm ×4．2 mm ，5μm）주위색보주，이사경부남‐갑순‐30 mL · L －1삼불을산（61∶30∶9）위류동상，등도세탈，류속위1．0 mL · min－1，재기류속위2．5 mL · min－1，표이관온도시90℃；주온22℃，대첨채감성분진행정량분석。결과화청소류성분재2．725～27．25μg범위내구유량호적선성관계， r＝0．9999）；평균회수솔위101．6％，RSD위1．0％；화청소류성분지문도보유13개공유봉，방법학고찰결과량호，각산지흑과구기지문도상사도도대우0．99。첨채감재2．07～20．68μg범위내구유량호적선성관계（ r＝0．9997），평균회수솔위101．1％，RSD위1．4％。결론소건립적방법가고、은정，가용우흑과구기질량적전면평개。
Objective To establish the quality standard for Lycium ruthenicum Murray by HPLC .Methods The fingerprints and de‐termination of anthocyanins composition of Lycium ruthenicum Murray were performed by HPLC with C18 ODS 80 Ts QA (150 mm × 4 .9 mm ,7 μm) by gradient elution .The solvent system consisted of 100 mL · L -1 formic acid solution (1 mL · L -1 triflu‐oroacetic acid)‐acetonitrile(150 mL · L -1 methanol) .The detection wavelength was set at 525 nm ,the flow rate was 1 .0 mL · min-1 ,and the column temperature was maintained at 30 ℃ .The determination of betaine was performed by HPLC‐ELSD with Hypersil NH2 (250 mm × 4 .2 mm ,5 μm) column by isocratic elution .The solvent system consisted of tetrahydrofuran‐meth‐anol‐30 mL · L -1 trifluoroacetic acid(61∶30∶9) ,the flow rate of carrier gas was 2 .5 mL · min-1 ,the flow rate of solvent was 1 mL · min-1 ,drift tube temperature was 90 ℃ ,and the column temperature was maintained at 22 ℃ .Results The calibration curve of cyanidin‐3‐O‐glucoside chloride was linear in the range of 2 .725‐27 .25 μg (r=0 .999 9) .The average recovery rate was 101 .6% (n=6) ,with RSD 1 .0% .The calibration curve of betaine was linear in the range of 2 .07‐20 .68 μg (r =0 .999 7) .The average recovery rate was 101 .1% (n=6) ,with RSD 1 .4% .Conclusion The method is rapid ,accurate and reliable ,and the method can be used as the quality standard for L ycium ruthenicum M urray .