CAJ | 학술논문

目的研究新疆库尔勒香梨公梨母梨果肉中多糖的最佳提取工艺。方法以水为溶剂，采用水浴回流提取，紫外‐可见分光光度法测定库尔勒香梨公梨母梨果肉中多糖的含量，以多糖含量为指标，考察提取温度、料液比、提取时间、提取次数对库尔勒香梨公梨母梨果肉中多糖提出率的影响。对照品溶液和供试品溶液均在621 nm处有最大吸收，回归方程：Y＝51．492 X－0．0107，r＝0．9998。结果最终确定库尔勒香梨公梨果肉多糖的最佳提取条件为：料液比1∶60，提取时间180 min ，提取温度70℃，提取次数为3次；母梨果肉多糖的最佳提取条件为果肉多糖的最佳提取条件为：料液比1∶60，提取时间60 min ，提取温度60℃，提取次数为1次。库尔勒香梨公梨母梨果肉平均多糖得率分别为70．03和78．29 mg · g －1。结论该方法简单易行，结果准确，适用于库尔勒香梨中总多糖的含量测定。
목적연구신강고이륵향리공리모리과육중다당적최가제취공예。방법이수위용제，채용수욕회류제취，자외‐가견분광광도법측정고이륵향리공리모리과육중다당적함량，이다당함량위지표，고찰제취온도、료액비、제취시간、제취차수대고이륵향리공리모리과육중다당제출솔적영향。대조품용액화공시품용액균재621 nm처유최대흡수，회귀방정：Y＝51．492 X－0．0107，r＝0．9998。결과최종학정고이륵향리공리과육다당적최가제취조건위：료액비1∶60，제취시간180 min ，제취온도70℃，제취차수위3차；모리과육다당적최가제취조건위과육다당적최가제취조건위：료액비1∶60，제취시간60 min ，제취온도60℃，제취차수위1차。고이륵향리공리모리과육평균다당득솔분별위70．03화78．29 mg · g －1。결론해방법간단역행，결과준학，괄용우고이륵향리중총다당적함량측정。
Objective To study on the optimal extraction technology of polysaccharides in Xinjiang male and female Korla pear pulps .Methods Water as solvent ,water bath reflux as extraction method ,UV‐visible spectrophotometry as determination method , and polysaccharide content as an indicator ,the extraction temperature ,solid‐liquid ratio and extraction time were investigated .The detection wavelength was 621 nm .The regression equation was Y = 51 .492X -0 .010 7 ,r=0 .999 8 .Results The polysaccha‐rides in male pears optimal extraction conditions were as follows :liquid ratio of 1∶60 ,extraction time 180 min ,extraction temper‐ature 70 ℃ ,extraction times thrice .The optimal extraction conditions for polysaccharides in female pears were as follows :solid‐liquid ratio 1∶60 ,extraction time 60 min ,extraction temperature 60 ℃ ,extraction times once .The average yield of polysaccha‐rides of male and female pears were 70 .03 and 78 .29 mg · g -1 ,respectively .Conclusion This method is simple ,reproducible , sensitive and can be used to determine the total polysaccharides in Korla pears .