中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2015年
4期
533-536
,共4页
钟敏%戴满花%刘爱玲%李毕华%黄为群
鐘敏%戴滿花%劉愛玲%李畢華%黃為群
종민%대만화%류애령%리필화%황위군
糖尿病,妊娠/代谢%血清淀粉样蛋白A/代谢%脂肪组织/代谢%胰岛素抗药性%体质量
糖尿病,妊娠/代謝%血清澱粉樣蛋白A/代謝%脂肪組織/代謝%胰島素抗藥性%體質量
당뇨병,임신/대사%혈청정분양단백A/대사%지방조직/대사%이도소항약성%체질량
Diabetes,gestational/ME%Serum amyloid A protein/ME%Adipose tissue/ME%Insulin resistance%Body weight
目的 探讨血清淀粉样蛋白A(SAA)在妊娠期糖尿病脂肪组织中的表达及与胰岛素抵抗和体质量指数的关系.方法 选取单胎足月剖宫产分娩的妊娠期糖尿病孕妇30例为研究组,随机选取同期住院分娩的糖耐量正常孕妇30例为对照组.根据身高、体质量计算孕前、分娩时体质量指数及孕期体质量增长值;采用氧化酶法检测空腹血糖(FBG),放射免疫法检测空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);ELISA法检测孕妇血清SAA水平;RT-PCR检测内脏及皮下脂肪组织中SAA1的mRNA的表达丰度;比较两组孕妇内脏脂肪及皮下脂肪组织的SAA1 mRNA表达量,分析其与血清的SAA、HOMA-IR及体质量指数等各项指标的关系.结果 妊娠糖尿病(GDM)组的内脏及皮下脂肪组织SAA1mRNA的表达丰度(0.447±0.069,0.291±0.067)高于对照组(0.194±0.070,0.231±0.068,P＜0.01),血清SAA水平[(21.038±6.648)mg/L]及HOMA-IR(4.168±2.416)高于对照组[(14.384±12.770) mg/L,2.045±1.008,P＜0.05];60例孕妇内脏及皮下脂肪组织SAA1 mRNA表达丰度与血清SAA水平呈正相关(r=0.353,0.342,P＜0.01);内脏脂肪组织SAA1 mRNA表达丰度与孕前及分娩时体质量指数、孕期体质量增长及HOMA-IR呈正相关(r=0.543,0.644,0.340,0.473,P＜0.01),皮下脂肪组织SAA1mRNA与孕前、分娩时体质量指数及HOMA-IR呈正相关(r=0.788,0.693,0.504,P＜0.01),而与孕期体质量增长无相关(r=0.013,P＞0.05).结论 SAA1 mRNA在GDM患者皮下及内脏脂肪组织表达上调,与体质量指数及胰岛素抵抗密切相关,可能通过增加胰岛素抵抗参与GDM的形成,可作为GDM的一种新的监测因子.
目的 探討血清澱粉樣蛋白A(SAA)在妊娠期糖尿病脂肪組織中的錶達及與胰島素牴抗和體質量指數的關繫.方法 選取單胎足月剖宮產分娩的妊娠期糖尿病孕婦30例為研究組,隨機選取同期住院分娩的糖耐量正常孕婦30例為對照組.根據身高、體質量計算孕前、分娩時體質量指數及孕期體質量增長值;採用氧化酶法檢測空腹血糖(FBG),放射免疫法檢測空腹胰島素(FINS)水平,計算胰島素牴抗指數(HOMA-IR);ELISA法檢測孕婦血清SAA水平;RT-PCR檢測內髒及皮下脂肪組織中SAA1的mRNA的錶達豐度;比較兩組孕婦內髒脂肪及皮下脂肪組織的SAA1 mRNA錶達量,分析其與血清的SAA、HOMA-IR及體質量指數等各項指標的關繫.結果 妊娠糖尿病(GDM)組的內髒及皮下脂肪組織SAA1mRNA的錶達豐度(0.447±0.069,0.291±0.067)高于對照組(0.194±0.070,0.231±0.068,P＜0.01),血清SAA水平[(21.038±6.648)mg/L]及HOMA-IR(4.168±2.416)高于對照組[(14.384±12.770) mg/L,2.045±1.008,P＜0.05];60例孕婦內髒及皮下脂肪組織SAA1 mRNA錶達豐度與血清SAA水平呈正相關(r=0.353,0.342,P＜0.01);內髒脂肪組織SAA1 mRNA錶達豐度與孕前及分娩時體質量指數、孕期體質量增長及HOMA-IR呈正相關(r=0.543,0.644,0.340,0.473,P＜0.01),皮下脂肪組織SAA1mRNA與孕前、分娩時體質量指數及HOMA-IR呈正相關(r=0.788,0.693,0.504,P＜0.01),而與孕期體質量增長無相關(r=0.013,P＞0.05).結論 SAA1 mRNA在GDM患者皮下及內髒脂肪組織錶達上調,與體質量指數及胰島素牴抗密切相關,可能通過增加胰島素牴抗參與GDM的形成,可作為GDM的一種新的鑑測因子.
목적 탐토혈청정분양단백A(SAA)재임신기당뇨병지방조직중적표체급여이도소저항화체질량지수적관계.방법 선취단태족월부궁산분면적임신기당뇨병잉부30례위연구조,수궤선취동기주원분면적당내량정상잉부30례위대조조.근거신고、체질량계산잉전、분면시체질량지수급잉기체질량증장치;채용양화매법검측공복혈당(FBG),방사면역법검측공복이도소(FINS)수평,계산이도소저항지수(HOMA-IR);ELISA법검측잉부혈청SAA수평;RT-PCR검측내장급피하지방조직중SAA1적mRNA적표체봉도;비교량조잉부내장지방급피하지방조직적SAA1 mRNA표체량,분석기여혈청적SAA、HOMA-IR급체질량지수등각항지표적관계.결과 임신당뇨병(GDM)조적내장급피하지방조직SAA1mRNA적표체봉도(0.447±0.069,0.291±0.067)고우대조조(0.194±0.070,0.231±0.068,P＜0.01),혈청SAA수평[(21.038±6.648)mg/L]급HOMA-IR(4.168±2.416)고우대조조[(14.384±12.770) mg/L,2.045±1.008,P＜0.05];60례잉부내장급피하지방조직SAA1 mRNA표체봉도여혈청SAA수평정정상관(r=0.353,0.342,P＜0.01);내장지방조직SAA1 mRNA표체봉도여잉전급분면시체질량지수、잉기체질량증장급HOMA-IR정정상관(r=0.543,0.644,0.340,0.473,P＜0.01),피하지방조직SAA1mRNA여잉전、분면시체질량지수급HOMA-IR정정상관(r=0.788,0.693,0.504,P＜0.01),이여잉기체질량증장무상관(r=0.013,P＞0.05).결론 SAA1 mRNA재GDM환자피하급내장지방조직표체상조,여체질량지수급이도소저항밀절상관,가능통과증가이도소저항삼여GDM적형성,가작위GDM적일충신적감측인자.
Objective To investigate the expression of serum amyloid A (SAA) in patients adipose tissue with gestational diabetes mellitus (GDM) and the correlations between SAA and insulin resistance (IR) and body mass index (BMI).Methods A total of 60 single full-term pregnant women underwent cesarean section from June 2013 to December 2013 was enrolled in this study (GDM group,n =30;control group,n =30);serum SAA level was detected with Enzyme-Linked Immunosorbent Assay (ELISA);and mRNA expression of SAA1 in adipose tissue was determined by reverse transcription PCR (RT-PCR);SPSS software was used to compare these markers,and the correlations between SAA and HOMA-IR,BMI were analyzed with Pearson correlation method.Results SAA,mRNA expressions in omental and subcutaneous fat in GDM group (0.447 ± 0.069,0.291 ± 0.067) were significantly higher than those in control group (0.194 ± 0.070,0.231 ± 0.068,P ＜ 0.01).Serum SAA levels [(21.038 ± 6.648) mg/L] and homeostasis model assessment of insulin resistance(HOMA-IR) (4.168± 2.416) in GDM group were significantly higher than those in control group [(14.384 ± 12.770) mg/L,2.045 ± 1.008,P ＜ 0.05];SAA1 mRNA expression levels in omental and subcutaneous fat were positively correlated with serum SAA (r =0.353,0.342,P ＜ 0.01).SAA1 mRNA expression levels in omental were positively correlated to pregestational BMI,late gestational BMI,weight gain in pregnancy and HOMA-IR (r =0.543,0.644,0.340,0.473,P ＜ 0.01),and SAA1 mRNA expression levels in subcutaneous fat were positively correlated to pregestational BMI,late gestational BMI,and HOMA-IR (r =0.788,0.693,0.504,P ＜ 0.01),but was no correlation with weight gain in pregnancy(r =0.013,P ＞ 0.05).Conclusions SAA mRNA expressions in omental and subcutaneous fat in GDM group and serum SAA levels increase,which is positively correlated with BMI and the degree of insulin resistance,SAA may participate in the formation of GDM by increasing insulin resistance.SAA may be used as a new monitor of GDM.
