中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2015年
9期
2350-2352
,共3页
Kruppel样因子4%胃肿瘤%启动子%生物信息学
Kruppel樣因子4%胃腫瘤%啟動子%生物信息學
Kruppel양인자4%위종류%계동자%생물신식학
Kruppel like factor(KLF)4%Gastric cancer%Promoter%Bioinformatics
目的：检测Kruppel样因子KLF4基因在人胃癌细胞株MKN-45及人正常胃黏膜上皮细胞株GES-1的表达情况，并应用生物信息学方法分析KLF4基因启动子区序列，预测胃癌发生中其涉及的转录调控因子。方法采用实时荧光定量 PCR 方法检测 MKN-45及 GES-1中 KLF4 mRNA的表达；利用多种在线软件获取并分析人KLF4基因启动子序列，预测其转录因子结合位点和胃癌组织中相关的转录因子。结果实时定量PCR检测结果显示：KLF4 mRNA在人胃癌细胞株MKN-45中的表达低于在人正常胃黏膜细胞株 GES-1中的表达（4.24±0.15 vs 6.66±0.23）（ P＜0.01）；KLF4基因启动子序列全长为4915 bp，共涉及183个转录因子结合位点，在胃癌中异常表达的转录因子有17个，包括AP-1、AP-2、HIF-1、SP3等。结论 KLF4 mRNA在胃癌细胞株MKN-45中表达下调，生物信息学分析提示在胃癌发生中 AP-1、AP-2、HIF-1、SP3转录因子等可能参与调控KLF4的表达。
目的：檢測Kruppel樣因子KLF4基因在人胃癌細胞株MKN-45及人正常胃黏膜上皮細胞株GES-1的錶達情況，併應用生物信息學方法分析KLF4基因啟動子區序列，預測胃癌髮生中其涉及的轉錄調控因子。方法採用實時熒光定量 PCR 方法檢測 MKN-45及 GES-1中 KLF4 mRNA的錶達；利用多種在線軟件穫取併分析人KLF4基因啟動子序列，預測其轉錄因子結閤位點和胃癌組織中相關的轉錄因子。結果實時定量PCR檢測結果顯示：KLF4 mRNA在人胃癌細胞株MKN-45中的錶達低于在人正常胃黏膜細胞株 GES-1中的錶達（4.24±0.15 vs 6.66±0.23）（ P＜0.01）；KLF4基因啟動子序列全長為4915 bp，共涉及183箇轉錄因子結閤位點，在胃癌中異常錶達的轉錄因子有17箇，包括AP-1、AP-2、HIF-1、SP3等。結論 KLF4 mRNA在胃癌細胞株MKN-45中錶達下調，生物信息學分析提示在胃癌髮生中 AP-1、AP-2、HIF-1、SP3轉錄因子等可能參與調控KLF4的錶達。
목적：검측Kruppel양인자KLF4기인재인위암세포주MKN-45급인정상위점막상피세포주GES-1적표체정황，병응용생물신식학방법분석KLF4기인계동자구서렬，예측위암발생중기섭급적전록조공인자。방법채용실시형광정량 PCR 방법검측 MKN-45급 GES-1중 KLF4 mRNA적표체；이용다충재선연건획취병분석인KLF4기인계동자서렬，예측기전록인자결합위점화위암조직중상관적전록인자。결과실시정량PCR검측결과현시：KLF4 mRNA재인위암세포주MKN-45중적표체저우재인정상위점막세포주 GES-1중적표체（4.24±0.15 vs 6.66±0.23）（ P＜0.01）；KLF4기인계동자서렬전장위4915 bp，공섭급183개전록인자결합위점，재위암중이상표체적전록인자유17개，포괄AP-1、AP-2、HIF-1、SP3등。결론 KLF4 mRNA재위암세포주MKN-45중표체하조，생물신식학분석제시재위암발생중 AP-1、AP-2、HIF-1、SP3전록인자등가능삼여조공KLF4적표체。
Objective To explore the expression of KLF 4 gene in human gastric cancer cell lines MKN-45 and analyze the gene se-quence of KLF4 promoter region in gastric cancer .Methods Real-time PCR was used to detect KLF4 mRNA expression in MKN-45 and GES-1.A variety of online software were used to analyze the sequences of KLF 4 gene promoter and predict the binding sites of transcriptional factor and relevant transcription factors in gastric cancer .Results Real-time PCR showed that the KLF 4 mRNA level in MKN-45 was signif-icantly lower than that of GES-1(4.24±0.15 vs 6.66±0.23) with significant differences (P<0.01).The promoter region of KLF4 gene was 4 915 bp long,containing 183 transcriptional factors .Among them,17 transcriptional factors were abnormally expressed in gastric cancer ,in-cluding AP-1,AP-2,HIF-1,SP3,etc.Conclusions KLF4 mRNA expression is down-regulated in gastric cancer cell line MKN-45.Bioinfor-matics analysis shows that transcriptional factors AP-1,AP-2,HIF-1 and SP3 may be involved in regulation of KLF4 expression in the devel-opment of gastric cancer ,which provides theoretical basis for further research .
